1)primer sequence – refer table 1

2) type of DNA sample - Escherichia coli

3)melting temperature- Tm - annealing at 55C for 20 s

4)commercial name for DNA polymerase used - Taq polymerase

5) how many cycle of replication - 30 cycles

-2017948271

Siti Nur Aisya bt Ibrahim  
-2017197343
Nurul Nadia bt Kairuddin 
-
Raja Fairuz Shafizah bt Raja Mohamad Aznan

-2017997131

(1) primer sequence

-foward and reverse primer starts from 5’ to 3’

(2) type of dna sample

-blood 

-solitary of hair

(3) melting temperature 

-95 celcius

(4)commercial name dna polymerase

-Taq Dna Polymerase

(5)how many cycle replications

-20 to 40 cycles

Norazira Bt Mohd Yusof (2017552451)

 

 

Primer sequences - forward primer and reverse primer, 5’ to 3’

Types of DNA sample - blood

Melting temperature - 95 degree celcius

Commercial name for DNA polymerase used - Taq polymerase

How many cycle of replication - 30 cycles

2.Type of DNA sample: Cloned HIV DNA (HXB2)

3.Melting temperature: 95°C

4.Commercial name for DNA polymerase used: Thermus aquaticus (Taq) DNA polymerase and Thermotoga maritima (ULTMA™) DNA polymerase

5. How many cycle of replication: 35 cycles

Nur atikah hazirah bt zarimi (2017745517) 

Rohadatul aisy binti zolmi (2017172273) 

Siti nur aminara binti mohd azlan (2017153275) 

Nurul amirah binti mohamad farook (2017921391) 

 
 

1)      DNA primer sequence- Standard primer PCR reactions were performed using the c and f primers (5′-GCTCAGCTCCGTTTCGGTTTCACTTCCGGT-3′; 5′-AGCCCCGCACTTCCACCACCAGCTCCTCCA-3′, respectively) 

 

2)      Type of DNA sample- 1% of the DNA from a single dried blood spot. 

 

3)      Melting temperature- After denaturation (boiling for 15 minutes), the probe was blocked with Cot1 DNA (Invitrogen) for 20 minutes at 65°C. 

 

4)      Commercial name for DNA polymerase use- Chimeric PCR primer 

 

5)      How many cycle of replication- The PCR cycling profile was as follows: denaturation at 98°C for 10 minutes, 10 cycles at 97°C for 35 seconds, 64°C for 35 seconds, and 68°C for 4 minutes; 25 cycles at 97°C for 35 seconds, 64°C for 35 seconds, 68°C for 4 minutes plus a 20-second increment for each cycle; and a final extension at 68°C for 10 minutes. 

 

(2017132381)

2. Azyan Anisah Binti Ramli (2017998411)

3. Nurul Nadiah Binti Ismail (2017143103)

5. Nisa' Marini binti Abdul Jamil (2017337959)


1) PRIMER sequence: 5’ to 3’ and 3’ to 5’ sequence ( A sequence that complementary to the template)
2) Type of DNA sample: Bacterial DNA
3) Melting Temperature: 95 degree celcius.
4) Commercial name for DNA polymerase used: Taq DNA Polymerase
5) How many cycle of replication: can be up to 40 cycle.

2.Fauzan Haziq B. Mardin (2017148657)

3. Muhammad Hasni B. Rosli (2017134357)

4.Ika Cemalia Bt. Ahmad Rohimin (2017501647)

-          NUR AQILAH BINTI MOHAMAD RADZI (2019707221)

-          NURUL HAFIZA BINTI HJ. MOHD NAJIB (2019726035)

-          SYAHIDATUL FARAHUSNA BINTI MAZLAN (2019163229)

NOR KHAIRIYAH BINTI ZAMRI (2019336647)
NUR FATIHAH NAJIHA BINTI AZHAR (2019345893) 

2)      Muhammad Shamsuzzaman Bin Ilham Fitri (2019570651)

3)      Muhammd Soleh Bin Kamarulzaman (2018292358)

4)      Muhammad Luqmanulhakim Bin Aidil (2019705045)