by Matthew Caprice

Restriction Enzymes.

mattcaprice — 2015-11-10 20:14:27 UTC

By: Group 7: Matt, Myles, Tygan

Who made this discovery?

lazarusm8416 — 2015-11-10 20:16:31 UTC

Many molecular biologists contributed to the discovery of restriction enzymes. Werner Arber first hypothesized that the enzymes bind and cut DNA at specific sites. Hamilton O. Smith then verified Arber's hypothesis. Daniel Nathans pioneered the application of restriction enzymes in biotechnology.

Source: http://www.nobelprize.org/nobel_prizes/medicine/laureates/1978/press.html

When and Where was it Made?

mattcaprice — 2015-11-10 20:17:40 UTC

Restriction Enzymes were discovered and characterized in the late 1960s and early 1970s. The discovery was made in Switzerland, specifically at the University of Geneva, where Arber had attended.

Source: http://www.nature.com/scitable/spotlight/restriction-enzymes-18458113

Significance to Genetic Knowledge.

mattcaprice — 2015-11-10 20:36:23 UTC

Scientists now know that the bacteria uses the restriction enzymes to kill various viruses by cutting up the viral DNA. It also allows us to further understand genetics because they're used in many various experiments.

lazarusm8416 — 2015-11-10 20:36:59 UTC

lazarusm8416 — 2015-11-10 20:37:06 UTC

lazarusm8416 — 2015-11-10 20:37:26 UTC

Current uses of restriction enzymes

lazarusm8416 — 2015-11-10 20:50:25 UTC

Today, restriction enzymes are also used by scientists to cut DNA strands for many things including DNA cloning and DNA finger printing.

Sources: http://biotechlearn.org.nz/themes/dna_lab/restriction_enzymes

What is a Restriction Enzyme?

mattcaprice — 2015-11-10 20:50:33 UTC

Restriction enzymes are enzymes isolated from bacteria that cut the DNA to produce fragments, called restriction fragments. Restriction enzymes play a very important role in the construction of recombinant DNA molecules, as is done in gene cloning experiments. Another application of restriction enzymes is to map the locations of restriction sites in DNA.

Source:

http://www.phschool.com/science/biology_place/biocoach/red/intro.html

Why are restriction enzymes important.

lazarusm8416 — 2015-11-10 20:56:48 UTC

Restriction enzymes are a very important discovery because it furthered our knowledge on bacterium. More importantly however, they have become a basic tool in biotechnology, allowing many more experiments to be conducted in the future.