https://padlet.com/brediguieri/Bookmarks En: Dissertation developed to master's degree at PPGDI-UFES, by Bruno Carneiro Rediguieri. Advisor: Prof. Dr. Sarah Gonçalves Tavares. Illustrative images of slides made to the cytopathological examination using the imprint technique, associated with the Quick Panoptic staining method, with the description of different degrees of fungal load and associated inflammatory infiltrate. Pt: Dissertação de mestrado desenvolvida no PPGDI-UFES, por Bruno Carneiro Rediguieri. Orientadora: Profa. Dra. Sarah Gonçalves Tavares. Fotos ilustrativas de lâminas de exame citopatológico, produzidas usando a técnica de imprint associada ao método de coloração de Panótico Rápido, com a descrição dos diferentes graus de carga fúngica e de infiltrado inflamatório associado. en-us 2020-07-17 00:34:56 UTC 2025-09-29 02:00:36 UTC hello@padlet.com https://padlet.net/icons/png/1f52c.png brediguieri https://padlet.com/brediguieri/Bookmarks/wish/658842183 En: Presence of yeasts suggestive of Sporothrix (oval, rounded or "cigar-shape" elongated structures with an uncolored external halo) with a moderate fungal load, predominantly within macrophages (red arrows) and more rarely in the extracellular medium (black arrows). Intense inflammatory cells infiltration, mainly neutrophils (yellow arrow). Magnification 100X. Pt: Presença de leveduras sugestivas de Sporothrix (ovais, arredondadas ou alongadas em formato de “charuto”  com halo externo não corado), com moderada carga fúngica, predominantemente dentro de macrófagos (setas vermelhas) e mais raramente no meio extracelular (setas pretas). Intenso infiltrado de células inflamatórias, principalmente neutrófilos (seta amarela). Aumento 100X. ]]> 2020-07-21 02:01:02 UTC https://padlet.com/brediguieri/Bookmarks/wish/658842183 brediguieri https://padlet.com/brediguieri/Bookmarks/wish/658856833 En: Presence of yeasts suggestive of Sporothrix (oval, rounded or "cigar-shape" elongated structures with an uncolored external halo) with a high fungal load, predominantly in the extracellular medium (black arrows). Moderate inflammatory cells infiltration (yellow arrows). Magnification 100X. Pt: Presença de leveduras sugestivas de Sporothrix (ovais, arredondadas ou alongadas em formato de “charuto”  com halo externo não corado), com alta carga fúngica, predominantemente no meio extracelular (setas pretas). Moderado infiltrado de células inflamatórias (setas amarelas). Aumento 100X. ]]> 2020-07-21 02:18:28 UTC https://padlet.com/brediguieri/Bookmarks/wish/658856833 brediguieri https://padlet.com/brediguieri/Bookmarks/wish/658865515 En: Presence of yeasts suggestive of Sporothrix (oval, rounded or "cigar-shape" elongated structures with an uncolored external halo) with a high fungal load, predominantly in the extracellular medium (black arrows).  Rare inflammatory cells (neutrophils, yellow arrows). Magnification 100X. Pt: Presença de leveduras sugestivas de Sporothrix (ovais, arredondadas ou alongadas em formato de “charuto”  com halo externo não corado), com alta carga fúngica, predominantemente no meio extracelular (setas pretas). Raro infiltrado de células inflamatórias (neutrófilos, setas amarelas). Aumento 100X. ]]> 2020-07-21 02:29:05 UTC https://padlet.com/brediguieri/Bookmarks/wish/658865515 brediguieri https://padlet.com/brediguieri/Bookmarks/wish/658872638 En: Presence of yeasts suggestive of Sporothrix (oval, rounded or "cigar-shape" elongated structures with an uncolored external halo) with a high fungal load, predominantly in the extracellular medium (black arrows), associated with an intense inflammatory cells infiltrate (yellow arrows). Magnification 40X.
Pt: Presença de leveduras sugestivas de Sporothrix (ovais, arredondadas ou alongadas em formato de “charuto”  com halo externo não corado), com alta carga fúngica, predominantemente no meio extracelular (setas pretas), associadas a um intenso infiltrado de células inflamatórias (setas amarelas). Aumento 40X.]]> 2020-07-21 02:37:18 UTC https://padlet.com/brediguieri/Bookmarks/wish/658872638 brediguieri https://padlet.com/brediguieri/Bookmarks/wish/658882585 En: Presence of yeasts suggestive of Sporothrix (oval, rounded or "cigar-shape" elongated structures with an uncolored external halo) with moderate fungal load, predominantly within macrophages (black arrow) and more rarely in the extracellular medium (red arrow). We can also observe a moderate inflammatory cells infiltration (neutrophils, yellow arrows). Magnification 100X. Pt: Presença de leveduras sugestivas de Sporothrix (ovais, arredondadas ou alongadas em formato de “charuto”  com halo externo não corado), com moderada carga fúngica, predominantemente no interior de macrófagos (seta preta) e mais raramente no meio extracelular (seta vermelha). Podemos observar também um moderado infiltrado de células inflamatórias (neutrófilos, setas amarelas). Aumento 100X.]]> 2020-07-21 02:48:32 UTC https://padlet.com/brediguieri/Bookmarks/wish/658882585 brediguieri https://padlet.com/brediguieri/Bookmarks/wish/658892526 En: Presence of yeasts suggestive of Sporothrix (oval, rounded or "cigar-shape" elongated structures with an uncolored external halo) with moderate fungal load, predominantly within macrophages (black arrow). We can also observe an intense infiltration of inflammatory cells (neutrophils, plasmocytes, degenerate neutrophils - red arrows). Magnification 100X. Pt: Presença de leveduras sugestivas de Sporothrix (ovais, arredondadas ou alongadas em formato de “charuto”  com halo externo não corado), com moderada carga fúngica, predominantemente dentro de macrófagos (seta preta). Podemos observar também um intenso infiltrado de células inflamatórias (neutrófilos, plasmócito, neutrófilos degenerados - setas vermelhas). Aumento 100X. ]]> 2020-07-21 03:00:39 UTC https://padlet.com/brediguieri/Bookmarks/wish/658892526 brediguieri https://padlet.com/brediguieri/Bookmarks/wish/658900580 En: Macrophage full of yeasts suggestive of Sporothrix (oval, rounded or "cigar-shape" elongated structures with an uncolored external halo). Magnification 100X. Enlarged photo. Pt: Macrófago repleto de leveduras sugestivas de Sporothrix (ovais, arredondadas ou alongadas em formato de “charuto”  com halo externo não corado). Aumento 100X. Foto ampliada.]]> 2020-07-21 03:11:15 UTC https://padlet.com/brediguieri/Bookmarks/wish/658900580 brediguieri https://padlet.com/brediguieri/Bookmarks/wish/658910660 En: Presence of yeasts suggestive of Sporothrix (oval, rounded or "cigar-shape" elongated structures with an uncolored external halo), moderate fungal load, predominantly in the extracellular medium (black arrows). We can observe a rare inflammatory cells infiltration (red arrows), in addition to a marked presence of red blood cells (yellow arrows). Magnification 100X. Pt: Presença de leveduras sugestivas de Sporothrix (ovais, arredondadas ou alongadas em formato de “charuto”  com halo externo não corado), moderada carga fúngica, predominantemente no meio extracelular (setas pretas). Podemos observar um raro infiltrado de células inflamatórias (setas vermelhas), além de presença acentuada de hemácias (setas amarelas). Aumento 100X. ]]> 2020-07-21 03:25:49 UTC https://padlet.com/brediguieri/Bookmarks/wish/658910660 brediguieri https://padlet.com/brediguieri/Bookmarks/wish/658915463 En: Presence of few budding yeasts, suggestive of Sporothrix. Note that there are two yeasts, an oval and a rounded one, with an uncolored outer halo around (black arrow). We can observe a low fungal load and a very intense inflammatory cell infiltration (yellow arrows). Magnification 100X. Pt: Presença de escassas leveduras em brotamento, sugestivas de Sporothrix. Note que há duas leveduras, uma oval e outra arredondada, com um halo externo não corado em volta (seta preta). Podemos observar uma baixa carga fúngica e um infiltrado de células inflamatórias (setas amarelas) muito intenso. Aumento 100X.]]> 2020-07-21 03:32:43 UTC https://padlet.com/brediguieri/Bookmarks/wish/658915463 brediguieri https://padlet.com/brediguieri/Bookmarks/wish/658921231 En: Presence of unique rounded shape yeast with an uncolored external halo, suggestive of Sporothrix (black arrow) within macrophages. Rare inflammatory cells (yellow arrow). Magnification 100X. Pt: Presença de uma única levedura arredondada, com halo externo não corado, sugestiva de Sporothrix (seta preta), no interior de um macrófago. Raras células inflamatórias (seta amarela). Aumento 100X. ]]> 2020-07-21 03:39:30 UTC https://padlet.com/brediguieri/Bookmarks/wish/658921231 sarahunifesp https://padlet.com/brediguieri/Bookmarks/wish/665344122 En: For cytopathological evaluation, a slide imprint of the lesion suspected of sporotrichosis was performed. Then, the slide was dried at room temperature, fixed and stained by the Quick Panoptic method (Kit InstantProv; Newprov), according to the manufacturer's recommendations. Therefore, the slide was submerged in solution no.1, maintaining a continuous movement from top to bottom, for 5 seconds (5 immersions of 1 second each). Then, the same procedure was performed in solutions no. 2 and no. 3. Finally, the slide was washed with abundant running water to remove excess dye and dried at room temperature, in the vertical position and with the imprint spot facing up. Pt: Para a avaliação citopatológica, foi realizado um imprint em lâmina da lesão suspeita de esporotricose. Em seguida, a lâmina foi seca em temperatura ambiente, fixada e corada pelo método Panótico Rápido (Kit InstantProv; Newprov), de acordo com as recomendações do fabricante. Sendo assim, a lâmina foi submersa na solução nº 1, mantendo-se um movimento contínuo de cima para baixo, durante 5 segundos (5 imersões de 1 segundo cada). Em seguida, realizou-se o mesmo procedimento nas soluções nº 2 e nº 3. Por fim, a lâmina foi lavada com água corrente abundante para retirada do excesso de corante e seca a temperatura ambiente, na posição vertical e com o local de imprint voltado para cima.

]]> 2020-07-29 22:03:02 UTC https://padlet.com/brediguieri/Bookmarks/wish/665344122 brediguieri https://padlet.com/brediguieri/Bookmarks/wish/684127368 En: Absence of yeasts suggestive of Sporothrix (oval, rounded or "cigar-shape" elongated structures, with uncolored external halo). We can observe a very intense inflammatory cell infiltrate, including macrophage (black arrow), eosinophils (red arrows) and, predominantly, neutrophils (yellow arrows). Magnification 100X. Pt: Ausência de leveduras sugestivas de Sporothrix (ovais, arredondadas ou alongadas em formato de “charuto”,  com halo externo não corado). Podemos observar um infiltrado de células inflamatórias muito intenso, incluindo macrófago (seta preta), eosinófilos (setas vermelhas) e, predominantemente, neutrófilos (setas amarelas). Aumento 100X. ]]> 2020-08-18 02:31:13 UTC https://padlet.com/brediguieri/Bookmarks/wish/684127368