https://padlet.com/chan37921/tutorial2 Gorello, P., et al., 2006. Quantitative assessment of minimal residual disease in acute myeloid leukemia carrying nucleophosmin (NPM1) gene mutations. Leukemia, 20, pp. 1103-1108. Available via: Nature [Accessed 28/11/2016]. en-us 2016-11-30 01:01:57 UTC 2023-01-29 21:19:24 UTC hello@padlet.com https://padlet-assets.s3.amazonaws.com/icons/File.png chan37921 https://padlet.com/chan37921/tutorial2/wish/140810298 2016-11-30 14:21:46 UTC https://padlet.com/chan37921/tutorial2/wish/140810298 chan37921 https://padlet.com/chan37921/tutorial2/wish/140814399 to produce reliable, sensitive RQ-PCR assays for NPM1 mutations that monitor and quantify MRD in AML patients with normal karyotype and NPM1 gene mutations.

]]> 2016-11-30 14:31:06 UTC https://padlet.com/chan37921/tutorial2/wish/140814399 chan37921 https://padlet.com/chan37921/tutorial2/wish/141063195 Materials:

• Bone marrow and/or peripheral blood mononuclear cells subjected to RNA & DNA extraction
• Plasmids

Molecular Technique:

• Real-time quantitative (RQ) Polymerase Chain Reaction (PCR) assays

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Monitoring of MRD in NPMc+ acute myeloid leukemia
At diagnosis, more than 55 000 mutated copies were found in all but one  of these 13 cases. After induction therapy, the number of copies dropped markedly in 10 patients who achieved complete haematological remission. A small or no decrease in NPM1-mutated copies was observed in three patients. In the other two, the number of copies rose again and haematological relapse was diagnosed at different time-points.

Conclusion:
The specific, sensitive RQ-PCR assays for cDNA and genomic DNA quantitatively assess NPM1-mutated gene copies and provide a reliable system for monitoring MRD in the majority of AML patients with normal karyotype.


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