Northeast ASK BIO Workshop Breakout Room 1A by ASK BIO team https://padlet.com/askbio/k763d32yyr0i09l1 Summer 2021 Assessment items en-us 2021-07-30 23:23:09 UTC 2023-06-28 04:02:54 UTC hello@padlet.com Adding a new post askbio https://padlet.com/askbio/k763d32yyr0i09l1/wish/1665274169 Click on the (+) symbol. 
You can copy your text in the box and even add images. (use the up arrow below to add images to your question]]> 2021-07-30 23:23:09 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1665274169 askbio https://padlet.com/askbio/k763d32yyr0i09l1/wish/1665274170 2021-07-30 23:23:09 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1665274170 Homework askbio https://padlet.com/askbio/k763d32yyr0i09l1/wish/1665276196
  • Consider some of your own test items.  
  • Use the item writing guidelines and work to improve TWO of your items. 
  • Under your name (Q1 & Q2), post your original question and then copy and paste the revised question from Google Docs.
  • The Feedback column will be empty.

    • ]]>     2021-07-30 23:31:25 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1665276196     Original Question askbio https://padlet.com/askbio/k763d32yyr0i09l1/wish/1665278812 2021-07-30 23:41:48 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1665278812 Revised Question askbio https://padlet.com/askbio/k763d32yyr0i09l1/wish/1665961320 2021-08-01 15:47:27 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1665961320 cell biology https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670055871 A major difference between the way a protein crosses mitochondrial membranes compared to the way a protein          crosses the nuclear envelope is that a protein that crosses the mitochondrial membrane ______.
    a) always keeps its signal sequence after it crosses
    b) always crosses one membrane at a time               
    *c) must unfold to be translocated
    d) enters the mitochondrial matrix in the form of a membranous vesicle
    e) uses mitochondrial ribosomes to finish its translation once across   
    ]]>     2021-08-05 14:16:18 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670055871     cell biology https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670058730 In a water environment, “detergents” tend to form “micelles” instead of bilayers primarily because detergents ___
    a) are non-polar lipids and bilayers can only be formed by dipolar lipids
    b) completely dissociate into ions during micelle formation whereas bilayers do not
    c) are purely lipid in nature and only lipid-protein complexes can form bilayers   
    *d) have only 1 non-polar tail while membrane lipids that form bilayers have 2 non-polar tails
    e) lack the phosphate groups necessary for bilayer formation
     
    ]]>     2021-08-05 14:19:03 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670058730     The four main ingredients in a Polymerase Chain Reaction are https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670097144  i.Taq polymerase
     ii.dNTPs
    iii.Primers
    iv.Template

    Based on your understanding of PCR, which ingredients (see list above) are used up during a PCR?
    a. ii only
    b. i  (Taq Polymerase) and ii (dNTPs)
    c. ii (dNTPs) and iii (Primers) * Answer
    d. all of the ingredients]]>     2021-08-05 14:53:10 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670097144     DNA is generally amplified (either using PCR or using bacterial vectors) prior to sequencing. Which of the following best explains why? https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670099462 a. Just to be safe in case DNA is destroyed during the denaturation step during sequencing reactions
    b. So that we have a higher chance of ssDNA coming in contact with sequencing DNA polymerase
    c. More DNA molecules makes it easier to read the gel
    d. Amplification increases the probability of getting all possible chain terminated products * Answer]]>     2021-08-05 14:55:16 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670099462     https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670111078 2021-08-05 15:07:07 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670111078 https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670113242 2021-08-05 15:09:05 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670113242 https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670222994 The graphic is very clear, and I like the way it is labled. However, I am not sure what the choices A, B, and C should read. Is there information missing? ]]> 2021-08-05 17:14:00 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670222994 Original Question https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670225013 Course: 300 level genetics lab
    Learning Objective: Apply the chemistry of DNA replication (not in list)
    BioSkill: Model application/Connecting scientific knowledge
    Question Format Type: Complex Multiple Choice
    Question Stem
    The four main ingredients in a Polymerase Chain Reaction are i.Taq polymerase ii.dNTPs iii.Primers iv.Template.  
    Based on your understanding of PCR, which ingredients (see list above) are used up during a PCR? 
    a. ii only 
    b. i and ii 
    c. ii and iii 
    d. all of the ingredients

    Correct Answer(s) (if closed response)c. ii and iii
    Explanation of correct/incorrect answer(s) Students have to apply knowledge from 100 biology and chemistry (reactions & enzymes) and list what is the “enzyme” and which ingredients are the “reactants” and what is the product.
    Notes on this question to share with author:The main point I'm trying to get across here is that the primers are incorporated into product (most seem to miss this)]]>     2021-08-05 17:16:26 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670225013     Margaret Q1 emackster https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670226858 I wonder if you might be able to reduce the heafty reading load while maintaining the question's clarity. Something along the lines of "protein transport across the mitochondrial membrane differs from transport across the nuclear membrane. which of the following is true of mitochondrial membrane transport" it might even be able to be shorter. "crosses one membrane at a time" is confusing to me, do you mean the two membranes of the mitochondrion or that a protein doesn't cross the nuclear membrane and mitochondral membrane at the same time (which seems obvious?). aLl in all, a great and clear question, I think it could be shorter:).]]> 2021-08-05 17:18:37 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670226858 Adding the items in parentheses definitely helped me understand the options better! https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670226982 2021-08-05 17:18:47 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670226982 Margaret Q2 feedback emackster https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670228526 Why the quotes around detergents and micelles? are these vocab words that the students should know? Could you reduce the cognitive load of the question by offering 3 or 4 options instead of 5?]]> 2021-08-05 17:20:32 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670228526 https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670232292 I really like the diversity of concepts about water that this question asks! 
    My only concern is that the way it's laid out creates a high mental load in reading/interpreting each statement individually. That said, I'm really not sure how to improve it. Does anyone else have suggestions?]]>     2021-08-05 17:25:32 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670232292     https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670232845 The revised question is a lot more clear ask to what is being asked of students. Yesterday I think there was a recommendation to remove "all of the above" or "none of these" type answers, so maybe you could forego including the option d. 

    I'm also curious if it's possible to streamline this even more by just writing a. taq polymerase, b taq polymerase + dNTPS c. dNTPS + primers 

    ^^that kind of structure may avoid students mistaking what i ii iii and iv stand for and reduce the cognitive load of the question.]]>     2021-08-05 17:26:02 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670232845     sberke https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670232887 I like this question! My only comment is about option C: could a student who has some knowledge, but not perfect knowledge, get hung up on that one? If we're running a gel for PCR product then we do need enough DNA to visualize the bands, so is this option too much of an attractor? I don't teach sequencing myself, so maybe I am totally out in the wilderness here, just something to think about! :)]]> 2021-08-05 17:26:05 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670232887 https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670235379 I agree with the opinion that this question will really hone in on student knowledge about water.  
    I wonder if you might want to shorten the language on the last true/false for consistency with the other T/F items. ]]>     2021-08-05 17:29:30 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670235379     I guess something is missing or I am not understanding this question. Sorry https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670237685 2021-08-05 17:32:34 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670237685 https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670237819 I like the question but I agree with the other review to maybe eliminate all the Roman numerals and just use the words in the choices or stick to abc format]]> 2021-08-05 17:32:45 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670237819 Anna Q2 feedback emackster https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670239718 some suggestions for this wonderful question. The stem can just read "Identify all the true statements about water" and lose the rest. in the second option, eliminate the word "remaining" in the pH option, I would say "causing a pH change" the last option should become two different T/F options. Why are some words bolded?]]> 2021-08-05 17:35:04 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670239718 Feedback https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670240518 I really like these series of questions. This would be great in a formative /clicker question series, with only a few of them with things changed into the "true" version or vice versa for the summative assessment]]> 2021-08-05 17:36:04 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670240518 Thank you for writing this. I still had reservations for multiple TF questions but this question is a good example as to how one question can be used to cover a concept in details. Only suggession: I will preferably stick with 4 answer choices only https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670240590 2021-08-05 17:36:10 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670240590 https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670240971 Sounds like an interesting question but it does seem to be missing content in the answers? Also what exactly are we trying to assess here - polar vs nonpolar functional group?]]> 2021-08-05 17:36:42 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670240971 https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670245570 I also agree with the previous reviewer to just ask the question about whether each statement is a correct or incorrect property of water. The stem can be misleading that only some of the correct properties of water might apply to the specific statements made in the question ]]> 2021-08-05 17:42:20 UTC https://padlet.com/askbio/k763d32yyr0i09l1/wish/1670245570