(3B Closure) The Effects of Protein Mutations by Desirae House https://padlet.com/dhouse22/3BProteins To complete this padlet, refer to the directions here: https://docs.google.com/document/d/1qMormESiHeBN76tRSRC6mkxe1r2DKflj-GG_bzgCXGo/edit?usp=sharing en-us 2019-02-01 08:25:37 UTC 2025-06-14 16:00:20 UTC hello@padlet.com LaTerra Frechette https://padlet.com/dhouse22/3BProteins/wish/328430439 1. With the extra red there is not one to bind the extra red one with such as the first red one is binded with the blue. This technically makes it a positive net charge. With doing this I had to rearrange my proteins so that the white remains outside and the yellow remains on the inside and that the red and blue binded. 
2. When a protein is translated wrong it can affect its shape and function. This mutation can bad and allow something of the body to no longer function. With lysosomes if they are translated incorrectly the lysosomes does not function correctly. ]]> 2019-02-06 18:47:18 UTC https://padlet.com/dhouse22/3BProteins/wish/328430439 Margaret Greene https://padlet.com/dhouse22/3BProteins/wish/328430516 1. The mutation in the protein caused the chain to shift so that the red amino acid could be next to the blue. I had to re-bend the chain out of the middle of the protein towards the outside. 
2. Even when one amino acid is changed, the entire protein had to change shape. The shape of a protein is a factor in what function it plays. For example, if one amino acid is changed in an enzyme, the bind site could be completely changed. In the case of a lysosomes, when one amino acid is incorrect, the organelle doesn't function correctly because the protein isn't correctly bent or structured. This is is what causes pompe disease. Overall, amino acids can cause major change to protein shape and function.

]]> 2019-02-06 18:47:27 UTC https://padlet.com/dhouse22/3BProteins/wish/328430516 Zora Tyson 10026702 https://padlet.com/dhouse22/3BProteins/wish/328430933 1. The mutation (addition of the red bead in place of the yellow bead) changed the entire structure as the molecule had to bend in a different way in order for the newly placed red bead to be bunched with the other red and blue bead. This change in folding also changes its function.
2. The smallest change in the placement of amino acids in a molecule can complete change its function. In relation to the previous question the mutation (replacement of the yellow bead with the red) completely changed its folding thus the function was also changed.]]> 2019-02-06 18:48:03 UTC https://padlet.com/dhouse22/3BProteins/wish/328430933 Julian Hettler 00032799 https://padlet.com/dhouse22/3BProteins/wish/328431449 After]]> 2019-02-06 18:48:44 UTC https://padlet.com/dhouse22/3BProteins/wish/328431449 Scotty Moore https://padlet.com/dhouse22/3BProteins/wish/328431579 Before & After
1.  The mutation of the protein ultimately affected the overall structure and shape of the protein. Because of the addition of the red bead, suddenly the shape become more dense into the center of the protein. 
2. Change in structure of an enzyme changes the overall function of an enzyme. The change in structure changes the overall active site of an enzyme which affects if it will make reactions faster or not. ]]> 2019-02-06 18:48:58 UTC https://padlet.com/dhouse22/3BProteins/wish/328431579 Kennedy Perry 10029145 https://padlet.com/dhouse22/3BProteins/wish/328432385
  • The changing in the amino acid sequence on model affected the protein model by the mutation in the protein caused a shift in ionic molecules attached before. After the molecule then folded thus this is why the structure became much more spread out.
  • There are many possible effects on proteins. However most proteins will not work due to the fact that if does not have certain specific peptide bond. But if mutated in the wrong way the protein will not be able to function.
    • ]]>     2019-02-06 18:50:14 UTC https://padlet.com/dhouse22/3BProteins/wish/328432385     Daniel Gattman https://padlet.com/dhouse22/3BProteins/wish/328432388 After vs. Before
    1: the mutation in the protein caused the ionic molecules to attach earlier, and also added an extra charge into the mix. This caused the molecule to fold much differently, and for it's structure to become much more spread out. 
    2: A change in those enzymes could have a number of effects. Most likely, the protein will not work anymore and just be inert for it's intended purpose, but if mutated in the wrong way, it could start eating everything it comes across (including it's vesicle), and make the cell die. ]]>     2019-02-06 18:50:14 UTC https://padlet.com/dhouse22/3BProteins/wish/328432388     Nohely Sanchez https://padlet.com/dhouse22/3BProteins/wish/328432597 1. the mutation changed the structure of the protein. since the red had to be able to combine with the blue the protein had to reshape entirely in order to fulfill the protein folding rules. 
    2. A change in a amino acid means a change in the structure of the protein therefore giving the protein a different function, probably a function that is not to be executed. ]]>     2019-02-06 18:50:34 UTC https://padlet.com/dhouse22/3BProteins/wish/328432597     Aditya Valluri https://padlet.com/dhouse22/3BProteins/wish/328434515 1. The mutation in protein by caused the ionic molecules to shift and essentially reshape itself so the red could combine with the blue. This made me bend the protein in the middle. 
    2. The shape of the protein is what determines its use. A change in even one amino acid can change the function of the protein. In the example of lysosomes, if one amino acid is incorrect, the lysosomes does not function correctly because it is incorrectly structured or bent. This causes a multitude of ailments. ]]>     2019-02-06 18:53:33 UTC https://padlet.com/dhouse22/3BProteins/wish/328434515     Q1: The mutation in the protein caused the ionic molecules to shift and to reshape itself so the red could combine with the blue. This made me bend the protein in the middle. 00032799 https://padlet.com/dhouse22/3BProteins/wish/328435071 Q2-When one amino acid is changed, the whole sequence is changed which caused an incorrect function of the lysosomes.
    Before
    ]]>     2019-02-06 18:54:20 UTC https://padlet.com/dhouse22/3BProteins/wish/328435071     Lila Edmonds https://padlet.com/dhouse22/3BProteins/wish/328436521 Q1: The change affected my model because it switched one yellow amino for a red one and changed the whole sequence and structure. With that, I had to reshape the structure and put the new red amino acid with the other red and blue.
    Q2: If you change one amino acid then it 'changes the whole sequence. Therefore it gives the lysosomes an incorrect function and the organelle cannot function.]]>     2019-02-06 18:56:29 UTC https://padlet.com/dhouse22/3BProteins/wish/328436521     Danielle Eddy https://padlet.com/dhouse22/3BProteins/wish/328437918 Question 1: The change affected the amino acid because it caused a change in arrangement. I had to reshape the chain so the red could be near the blue.   
    Question Two: When one amino acid is changed, the whole sequence is changes causing an incorrect function of the lysosomes.]]>     2019-02-06 18:58:47 UTC https://padlet.com/dhouse22/3BProteins/wish/328437918     Hannah Shimmel https://padlet.com/dhouse22/3BProteins/wish/328441582 Q1. The mutation of the bead caused the entire protein to need to be refolded in order for both of the positive molecules could be next to the singular negative one. I had to refold the entire structure in order to accommodate the change.
    Q2. ]]>     2019-02-06 19:04:43 UTC https://padlet.com/dhouse22/3BProteins/wish/328441582     Rose Beacham https://padlet.com/dhouse22/3BProteins/wish/328468125
  • The mutation in the chain changed everything. It moved the new red to be with the blue, affecting the rest of the chain and where the other amino acids were placed.
  • With the newly changed amino acid, the entire protein chain’s structure changed. With this happening, it’s a possibility that the function of the protein could’ve easily changed as well because the function is based off of the structure.

    • ]]>     2019-02-06 19:51:16 UTC https://padlet.com/dhouse22/3BProteins/wish/328468125