DNA TECHNOLOGY GROUP WORK by NUR NAJMINA BINTI MOHD ZAKI Moe https://padlet.com/m9695637/jedyawyvujib7aaj by group 3 en-us 2021-11-04 10:29:00 UTC 2025-11-19 03:28:53 UTC hello@padlet.com https://padlet.net/icons/png/1f9eb.png 1. a) Define recombinant technology https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1867122427 The technology of preparing recombinant DNA in vitro by cutting up DNA molecules and splicing together fragments from more than one organism]]> 2021-11-04 10:53:39 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1867122427 1. b) Name the source of plasmid and what is the role in the cloning process? https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1867131464 Source : Bacteria cell
Role  : Can used in genetic engineering to amplify, or produce many copies of, certain genes.

  
]]> 2021-11-04 11:00:24 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1867131464 1. c) What is restriction enzyme m9695637 https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1867135568 An endonuclease that recognize and cleave DNA molecule at specific nucleotide sequences]]> 2021-11-04 11:03:33 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1867135568 2. a) https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1868993495 Q is most suitable to be a cloning vector.]]> 2021-11-05 02:38:07 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1868993495 2. b) https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1869028070 A suitable cloning vector is able to accept foreign DNA in multiple cloning sites (MCS).
Besides, they contain an origin of replication which they are able to replicate freely in the host cell. Moreover, they contain genetic markers for screening purpose such as ampR.]]> 2021-11-05 02:53:54 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1869028070 2. c) https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1869031682 Recombinant DNA]]> 2021-11-05 02:55:38 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1869031682 2. d) https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1869033944 Host cell]]> 2021-11-05 02:56:46 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1869033944 1. d) https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1869040882 Restriction site: GAATTC
Restriction enzyme: BamHl]]> 2021-11-05 03:00:10 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1869040882 1. e) https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1869055966 i. 4
ii. 4]]> 2021-11-05 03:07:53 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1869055966 1. f) https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1869057504 DNA Ligase]]> 2021-11-05 03:08:43 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1869057504 1. g) https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1869063905 Bacteria is suitable as a host cell due to the capable of receiving recombinant DNA through the transformation process. It is also able to maintain the structure of the recombinant DNA and amplify the gene product from recombinant DNA.]]> 2021-11-05 03:11:52 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1869063905 3. a) https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1872351239 EcoRI cleave and produce sticky end while SmaI cleave and produce blunt end. Besides, EcoRI cleave the restriction site between nucleotide G and A whereas SmaI cleave the restriction side between nucelotide G and C.]]> 2021-11-07 07:10:26 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1872351239 3. b) https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1872352948 i) staggered cut with single-stranded end.

ii) 5’ AATT 3’

iii) 2 pieces ]]> 2021-11-07 07:13:08 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1872352948 https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1872432767 2. e) The two characteristic of T are able to receive the recombinant DNA through the transformation process and able to express the gene product of the recombinant DNA .]]> 2021-11-07 09:00:43 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1872432767 m11489357 https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1872440647 2. f) No , it’s not possible to use different types of s for vector and target gene . It is because in order to produce compatible sticky ends , we need to cleaved with the same restriction enzymes. ]]> 2021-11-07 09:11:24 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1872440647 m11489357 https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1872454535 4. b) Bacteria that culture in medium that contains ampicillin will survive.]]> 2021-11-07 09:25:27 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1872454535 4. a) m11489357 https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1872455799 White and blue colonies are formed. White colonies indicated that the recombinant plasmid while the blue colonies indicated the non-recombinant plasmid.]]> 2021-11-07 09:26:54 UTC https://padlet.com/m9695637/jedyawyvujib7aaj/wish/1872455799