https://padlet.com/costanpj/ENVDNA The chemical observation of organisms present and past en-us 2017-02-24 23:02:28 UTC 2023-03-29 05:56:19 UTC hello@padlet.com https://padlet-assets.s3.amazonaws.com/icons/Xmastree.png costanpj https://padlet.com/costanpj/ENVDNA/wish/156208512 DNA is deoxyribonucleic acid, also known as the hereditary material in humans and almost all other organisms. DNA can be found in two places the cell nucleus (nuclear DNA), or mitochondria (mitochondrial DNA or mtDNA). DNA is made up of  four chemical bases: adenine (A), guanine (G), cytosine (C), and thymine (T). These base pairs also have partners. A hangs out with T and G partners up with C.

^{https://ghr.nlm.nih.gov/primer/basics/dna}]]> 2017-02-25 21:13:54 UTC https://padlet.com/costanpj/ENVDNA/wish/156208512 costanpj https://padlet.com/costanpj/ENVDNA/wish/156209455 EDNA refers to the genetic material that can be taken from large environmental samples such as soil, water, and even air. DNA is released into the environment through a variety of methods. These methods are excretion of feces, mucous, gametes, shed skin, hair and even from carcasses. The collection of eDNA samples is primarily done in bodies of water, to research species diversity and concentration. The average amount of time DNA will exist, free floating, through a body of water is 7-21 days. This depends on the movement of the water, concentration of the organism or if there has been a storm event.  _{http://freshwaterhabitats.org.uk}]]> 2017-02-25 21:36:39 UTC https://padlet.com/costanpj/ENVDNA/wish/156209455 costanpj https://padlet.com/costanpj/ENVDNA/wish/156212660

To detect secretive organisms in habitats (especially useful in aquatics).

Can be used to estimate the population of organisms in a habitat by DNA concentration.

Used to detect ancient species through DNA preserved in permafrost, sediments and ice cores.

]]> 2017-02-25 23:04:39 UTC https://padlet.com/costanpj/ENVDNA/wish/156212660 costanpj https://padlet.com/costanpj/ENVDNA/wish/156214769 The most important step in collecting eDNA is to ensure you don't contaminate your samples. Make sure that you and all of the sampling equipment are downstream of the chosen sampling location. Prime sampling locations are in the center of the current in small streams or in well mixed flow in larger streams. It is also necessary to make note of areas in the stream that would cause back-flow, such as a whirlpool. It is possible for your DNA to come off your clothing and contaminate the sample. It is also very important to ensure all the equipment used at a previous site has been thoroughly cleaned. If equipment is unclean DNA from previous sampling locations can contaminate future locations.]]> 2017-02-26 00:35:29 UTC https://padlet.com/costanpj/ENVDNA/wish/156214769 costanpj https://padlet.com/costanpj/ENVDNA/wish/156215306 The youtube channel Platypus SPOT provides a perfect example of obtaining samples manually. The alternative to using a syringe to collect water is a electronic pump that pulls the water through the filtering membrane. ]]> 2017-02-26 00:55:38 UTC https://padlet.com/costanpj/ENVDNA/wish/156215306 costanpj https://padlet.com/costanpj/ENVDNA/wish/156215695 Geotech Geopump™ Pumps is just one of the many companies that makes ground water pumps.

]]> 2017-02-26 01:10:26 UTC https://padlet.com/costanpj/ENVDNA/wish/156215695 costanpj https://padlet.com/costanpj/ENVDNA/wish/156217519 EDNA put into an ecosystem by reproduction and decomposition could produce similar temporal patterns despite originating from different events. Different filter types, with different particle filters, could yield different eDNA concentrations that reflect particle size of DNA captured, rather than population size differences of organisms. The resuspension of sedimentary eDNA could produce false positives of the presence of an organisms after they are long gone. Different environmentally-mediated eDNA decay rates could cause confusion about population size or biomass from eDNA concentration.
_{Barnes, M.A. & Turner, C.R. Conserv Genet (2016) 17: 1.} ]]> 2017-02-26 02:30:07 UTC https://padlet.com/costanpj/ENVDNA/wish/156217519 costanpj https://padlet.com/costanpj/ENVDNA/wish/156218798 Barnes, M.A. & Turner, C.R. Conserv Genet (2016) 17: 1. doi:10.1007/s10592-015-0775-4

Ficetola, Gentile Francesco; Miaud, Claude; Pompanon, François; Taberlet, Pierre (2008). "Species detection using environmental DNA from water samples". Biology Letters. 4 (4): 423–425. doi:10.1098/rsbl.2008.0118

http://www.geotechenv.com/peristaltic_geopump.html

https://www.fs.fed.us/research/genomics-center/docs/edna/edna-protocol.pdf

 What is DNA? - Genetics Home Reference. (n.d.). Retrieved February 19, 2017, from https://ghr.nlm.nih.gov/primer/basics/dna
 
“What is eDNA" Freshwater Habitats Trust.

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