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https://padlet.com/roberta_damiola/nano/wish/1909311318 roberta_damiola https://padlet.com/roberta_damiola/nano/wish/1918165314 cianobatteri come spirulina (coltivazione)anche educazione civica, ficobiliproteine (colorate e fluorescenti)  
ficocianina è il principale pigmento (blu e viene usata in alternativa ai coloranti artificiali E133 e E131) 

proteina nativa 625 colore blu - 370 nm incolore
fluorescenza 
estrazione e purificazione della ficocianina lisi cellulare (2,5g + 30ml) congelamento e scongelamento di 24-48 ore e spirulina+silice in 100ml di acqua  40minuti in ultrasuoni)
centrifugazione  4000 giri per 40 minuti (surnatante blu con fluorescenza viola) il surnatante continene la proteina e altri elementi
lampada di wood
spettro di assorbimento da 300 a 700nm A max 618nm

Saggi di denaturazione 2,5 ml di estratto con etanolo 15%,30%, 50%, 96%(diminuisce la polarità) e urea (si sostituisce alle molecole di acqua e...) spetto estratto acquosi 1:5

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