Mod three honors by Dev Chheda https://padlet.com/devmchheda/i7tzlatx8pml Liver enzyme lab en-us 2017-02-22 02:26:29 UTC 2017-02-22 02:28:38 UTC hello@padlet.com Module 3 Honors AssignmentTitle: Liver Enzyme Lab devmchheda https://padlet.com/devmchheda/i7tzlatx8pml/wish/155346734 Dev Chheda

Purpose

The purpose of this lab is to prove that the liver converts hydrogen peroxide into oxygen and water through a chemical reaction.

Introduction

Every cell in the human body creates hydrogen peroxide when it goes through a chemical reaction. However, peroxide is poisonous, so the human body has to be able to break down the peroxide. Catalase is also generated by every cell in the body, and it breaks down peroxide into oxygen and water.

Materials

Water
Hydrogen peroxide
Liver (pieces)’
Boiled liver
Matchs
Wooden splints
Adult supervision recommended

Procedure

  1. Fill the first test tube up to the two centimeter mark with water.
  2. Push a liver piece into the first test tube and observe for any reactions. This is the control group.
  3. Now, fill the second test tube up to the two centimeter mark with hydrogen peroxide.
  4. Push a liver piece into the second test tube and observe for any reactions. This is the “peroxide” group.
  5. Fill a third test tube with two centimeters of peroxide.
  6. Now light a wooden splint on fire, and let it burn for 5-10 seconds.
  7. Shake the flame out, and insert the glowing wooden splint into the third test tube. Observe to see if the peroxide reacts. This is used to prove that peroxide is not flammable.
  8. In a fourth test tube, repeat steps 3 and 4, and then repeat step 6.
  9. Now, insert the hot splint into the white substance that should be bubbling in the fourth test tube. Observe how the splint reacts to the substance.
  10. In a fifth test tube, insert a boiled liver into two centimeters of water. Now, add -peroxide and observe.

Results

Reactivity Results | Is there a reaction?
Test tube 1 (Control) | No
Test tube 2 (Peroxide) | Yes
Test tube 5 (Boiled liver) | No
In the control group, which has water and a liver piece, there is no reaction, but in the peroxide group, there is a reaction, meaning that the peroxide is what is causing the reaction with the liver.
Test tube 5 gives a demonstration that when enzymes are put outside of their temperature “comfort zone”, they will denature, or not work anymore.

Flammability Results | Is it flammable?
Test tube 3 (Control) | No
Test tube 4 (Liver/reaction) | Yes
In the control group, we can see that peroxide is not flammable. So, in the the liver group, we know that the substance created is flammable, and that pure oxygen is flammable, meaning that the reaction does in fact result in pure oxygen and water.

Discussion

  1. Enzymes are substances used to start and speed up chemical reactions inside an organism. I chemical reactions, enzymes play the role of the catalyst, or the substance that “ignites” (starts up) the reaction. Enzymes also speed up reactions inside organisms.
  2. When peroxide was added to the liver in test tube two, oxygen gas was produced, and water was left behind.
  3. The red hot splint ignites when it comes in contact with the substance in test tube 4., This is because the substance being created is oxygen gas, which is very flammable.
  4. Temperature can denature an enzyme if it is too high out of the enzyme’s “comfort zone”. This means that the enzyme will become less and less active until it is completely inactive.
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