My stunning padlet by Bivian Kathiana https://padlet.com/imbivian/hwfh1uzufb4l3z7f Made with a stroke of good luck en-us 2021-05-03 06:19:23 UTC 2024-06-03 10:52:16 UTC hello@padlet.com Team discussion 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572851068 If a transgenic triploid salmon escapes into the wild, what is/are the potential impact(s) on wild type salmon species?]]> 2021-05-31 05:48:05 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572851068 ANSWER to team discussion on impacts. imbivian https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572861624 1. disadvantage for the wild type salmon species since they will be outcompeted by the transgenic triploid salmons escapees. 

-transgenic triploid salmon escapees with a larger size
-transgenic triploid salmon has a higher feed conversion rate, requiring lesser food for growth compared to the wild type. 
-resident pathogens from the escapee's fish farm may be released into the wild, and since the transgenic triploid salmon are hardier, the wild types may be subject to an outbreak, lowering their survivability]]> 2021-05-31 05:52:47 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572861624 Traditional/selecting breeding 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572875028 2021-05-31 05:59:21 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572875028 Precise Generic Engineering/ Transgenics 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572877079 2021-05-31 06:00:28 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572877079 What is selective breeding? mdzy1184 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572889971 - A process used by humans to develop new organisms with desirable characteristics.
- Desirable characteristics such that to develop better or more useful strains from the genetic diversity. ]]> 2021-05-31 06:07:09 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572889971 Example of transgenic organism 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572903281 One example is the modification of the chicken genome to induce production of eggs containing a recombinant enzyme drug. After purification of the enzyme, this drug, marketed as Kanuma, can be used to treat people suffering from a rare condition wherein their body cannot break down fats with their normal enzyme, thus reducing fat accumulation in these individuals.
Recently, scientists used CRISPR to target genes encoding the proteins ovalbumin and ovomucoid, the common allergens in eggs. Researchers hope to produce hypoallergenic eggs in the future using this method.
]]> 2021-05-31 06:13:41 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572903281 Advantages of selective breeding mdzy1184 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572903640 - The traditional breeding can grow bigger and faster than the wild type. 
- Helps to eliminate disease (disease resistance) 
- It produces a fitter, hardier and stronger organisms
- It allows for higher profit 
- No  safety  issues such that it allows for natural evolutionary process to take place
- It is able to provide a sustainable food chain]]> 2021-05-31 06:13:51 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572903640 VS 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572904076 2021-05-31 06:14:04 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572904076 What is transgenics? 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572916446 The manipulation of an organism's genetic makeup using biotechnological tools]]> 2021-05-31 06:20:19 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572916446 Traditional breeding 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572923686 Traditional: done by crossing animals and selecting offspring.

]]> 2021-05-31 06:23:43 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572923686 Disadvantages of selective breeding mdzy1184 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572923833 - It can create offsprings with different traits 
- It may cause an environmental risk for the organisms (diseases and environmental changes) 
- There is no control over genetic mutations when it comes to bad traits. 
- It could potentially lead to loss in species variety.]]> 2021-05-31 06:23:48 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572923833 Transgenics 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572929401 Inserting selected genes using recombinant DNA methods]]> 2021-05-31 06:26:30 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572929401 Results in domesticated organisms 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572931473 2021-05-31 06:27:40 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572931473 Results in new traits in organism 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572932431 2021-05-31 06:28:10 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572932431 Making of GM salmon imbivian https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572934241 2021-05-31 06:29:10 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572934241 1. Growth hormone gene enhancement imbivian https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572937992 2021-05-31 06:31:00 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572937992 Example of selective breeding: Cow mdzy1184 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572938277 An example would include The Belgian Blue Cow. They have been selectively bred to maximize meat production since they contain a mutation which usually inhibits muscle production. The mutation switches the gene off, resulting in no limit on the amount of muscles these cow can form. ]]> 2021-05-31 06:31:10 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572938277 Affects 10k to 300k genes 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572938419 Desired gene(s) inserted with other genetic material inherited as well]]> 2021-05-31 06:31:15 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572938419 2. Sex reversal by testosterone imbivian https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572938956 2021-05-31 06:31:32 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572938956 3. Induction of triploidy imbivian https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572941434 2021-05-31 06:32:44 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572941434 Affects 1-4 genes 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572941490 Desired gene(s) inserted only at known locations. ]]> 2021-05-31 06:32:46 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572941490 Safety testing required 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572943648 especially on food safety]]> 2021-05-31 06:33:54 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572943648 For a mono-sex fish culture imbivian https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572944377 Young homozygous female transgenc fish treated with hormone to produce neomale fish

]]> 2021-05-31 06:34:15 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572944377 No safety test required 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572944892 2021-05-31 06:34:30 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572944892 How is this done? 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572947293 1. Isolate and transfer well defined genes
2. Introduction of desired genes across the species barrier
3. Desired change in 1 generation]]> 2021-05-31 06:35:48 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572947293 What is the effect? imbivian https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572950223 Neomale/Pseudomale Fish
Produces a genotypically female and a phenotypically male
: results in all female salmon. ]]> 2021-05-31 06:37:16 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572950223 Development of functional gene construct 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572955189 consists of:
-promoter sequence
-coding region
-terminator sequence
-(for plants selection, antibiotics resistance gene]]> 2021-05-31 06:39:50 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572955189 types of shock treatment and how it works 190155031 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572958741 Heat, Pressure, and Cold shocking
These methods are to shock a fertilized cell that has yet to go through meiosis II, causing the cell to halt meiosis. This causes a triploid cell to go through mitosis as it.]]> 2021-05-31 06:41:24 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572958741 Advantage of triploidy mdzy1184 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572959174 - Allows for beneficial commercial traits such as improved growth, pathogen resistance and infertility conferring protection of elite genetics. ]]> 2021-05-31 06:41:38 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572959174 Effects of triploidy mdzy1184 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572964669 The increase in cell size is compensated by a corresponding decrease in the number of cells. ]]> 2021-05-31 06:43:46 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572964669 How is triploidy achieved in salmon? mdzy1184 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572976656 A non-transgenic female eggs is fertilized by a transgenic neomale/pseudomale with the help of shock treatment, producing a sterile triploid all female salmon as a result.

]]> 2021-05-31 06:49:46 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572976656 Creating the transgenic brood stock 200613421 https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572984377 -Identify, clone gene of interest
-Link correct promoter to desired gene
-Linearize DNA construct
-inject linear DNA into just fertilized eggs via microinjection
- Salmon develops into adult with the trait being inheritable]]> 2021-05-31 06:53:42 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572984377 Effects that triploidy have on the testis and ovary imbivian https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572987637 It greatly affects the size and condition of it. Its visibly seen that the diploids organs are much better than the triploids. ]]> 2021-05-31 06:55:24 UTC https://padlet.com/imbivian/hwfh1uzufb4l3z7f/wish/1572987637