Research by Taylor Masters https://padlet.com/taylor_masters9/hjpc1x26inx9 Taylor Masters, Solene Bossart & Maggie Miller en-us 2018-09-17 20:40:37 UTC 2023-03-04 23:15:29 UTC hello@padlet.com Researching the efficacy of digestive supplements/over the counter drinks that boost digestive system and comparing them among each other taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/283125665 2018-09-18 19:17:20 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/283125665 something in the field of medicine, pharmaceutical, human behavior, public health taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/283126234 2018-09-18 19:18:30 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/283126234 https://docs.google.com/document/d/11UKcmewUPxMDPP0UcXImREV1tDNf7LDYIoPTVxwbwc4/edit?usp=sharing mam25326 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/287224693 2018-09-29 15:30:28 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/287224693 https://docs.google.com/document/d/10x3WS6hqW4vNLdKQB6dPAEVSdcD2Wc5Nq13oFfBfbYU/edit?usp=sharing solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/287853837
Rough draft of project proposal.]]> 2018-10-01 20:05:26 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/287853837 List of needed materials: taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/290100085 E. Coli - accepted
Lactobacillus - submitted
Kombucha - submitted
Apple Cider Vinegar - submitted
Swartz Probiotics - submitted]]> 2018-10-07 23:33:00 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/290100085 https://docs.google.com/document/d/1UR6BSZH93JqbUc_CeoPAXrChpKVYyB9wE1G8lc-ewWI/edit?usp=sharing solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/290101614 Final draft of project proposal.]]> 2018-10-07 23:50:08 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/290101614 (In Lab) taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/295689918 October 22: make procedures and finish planning

October 29: grow Lactobacillus and maybe E.Coli

November 5: Begin Project]]> 2018-10-22 20:27:44 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/295689918 Make sure you guys are adding to this each week! You could add photos of the MRS you made last week! hopebentley https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/300726570 2018-11-05 20:45:08 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/300726570 Today we faced many issues with our original procedures so the new procedures that will work are below. taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301313257 2018-11-07 02:57:22 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301313257 SETTING UP taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314086
  • Sterilize 20 test tubes and all beakers that will be used
  • Label 20 test tubes appropriately 
  • Add 4 mL of MRS into 10 test tubes
  • Add 4 mL of LB into the remaining 10 test tubes

    • ]]>     2018-11-07 03:01:47 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314086     MAKING PROBIOTIC SOLUTION taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314254
  • Heat up 200 mL of water on hot plate to 30 degree
  • add one pill in the water and turn on stir rod
  • Wait until completely dissolved

    • ]]>     2018-11-07 03:02:46 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314254     MAKING KOMBUCHA SOLUTION taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314325
  • Measure 200 mL of DI Water and add to sterilized beaker
  • Measure 100 mL of Kombucha and add to the beaker with water
  • Stir until solution is thoroughly mixed
    • ]]>     2018-11-07 03:03:11 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314325     MAKING ACV SOLUTION taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314408
  • Measure 200 mL of DI Water and add to sterilized beaker
  • Measure 100 mL of Apple Cider Vinegar (ACV) and add to the beaker with water
  • Stir until solution is thoroughly mixed
    • ]]>     2018-11-07 03:03:35 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314408     ADD BACTERIA TO MEDIA taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314557
  • Add 10 micro liters of lactobacilli to 10 MRS
  • Add 10 micro liters of E. coli to 10 LB
  • Stir each up and see if need to add more according to whats leftover 
    • ]]>     2018-11-07 03:04:23 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314557     ADD SOLUTIONS TO TUBES taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314617
  • Add 1 mL of water to 4 tubes
  • Stir Kombucha and add 1 mL to 4 tubes
  • Stir ACV and add 1 mL to 4 tubes
  • Stir dissolved probiotic solution and add 1 mL to 4 tubes 
  • Lastly, cover each test tube with parafilm and put in around 30 degree

    • ]]>     2018-11-07 03:04:47 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314617     taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314838 This is the diluted Kombucha and ACV made in lab]]> 2018-11-07 03:06:12 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314838 mam25326 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314849 - First, our probiotic pill did not dissolve in water as we thought that it would, so we had to work with it and find a way to force it to dissolve (we eventually got it to dissolve with continued stirring and heat)
    -We also used unsterile test tubes by accident, so after we filled them all with MRS broth and LB broth, we had to dump them out and start all over.

    ]]>     2018-11-07 03:06:18 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314849     Today we wrote our recipe-like procedures in our lab notebooks. taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314995 2018-11-07 03:07:09 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301314995 Our final orders came in and we are on track with our timeline to start the project next week on October 29th. taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301315086 2018-11-07 03:07:29 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301315086 Today in lab, we made the MRS broth. taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301315179 2018-11-07 03:08:04 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301315179 The google doc has the links to all of the articles used in our research. taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301315403 2018-11-07 03:09:19 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301315403 mam25326 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301315661 This is the probiotic pill the first time that we tried to dissolve it-- it clumped up and didn't dissolve. ]]> 2018-11-07 03:10:45 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301315661 Newest update: taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301315675 All items are now available]]> 2018-11-07 03:10:52 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301315675 mam25326 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301315712 Eventually with heat, stirring, and much more water, we got the probiotic pill to dissolve (pictured below). ]]> 2018-11-07 03:11:03 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301315712 We have noticed the large areas that could be affected by human error. taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301316396 2018-11-07 03:15:57 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301316396 1. The test tubes and beakers can very easily become unsterile taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301316412 2018-11-07 03:16:01 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301316412 2. The measured amounts of liquid are not always equal because we had to put in 1 mL 10 times to each test tube. taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301316500 2018-11-07 03:16:35 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301316500 taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301319681 2018-11-07 03:38:39 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301319681 making the MRS broth solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301739825 liquid nutrient broth (MRS- 10 test tubes)
    1. combine 55g of medium with one liter of demonized water 
    2. stir to mix throughly 
    3. boil to dissolve completely (do not overheat) 
    4. auto clave at 121C for 15 minutes ]]>     2018-11-07 19:17:54 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301739825     kombucha solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301740822 2018-11-07 19:19:46 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301740822 apple cider vinegar solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301741410 2018-11-07 19:20:51 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301741410 here is a to-do list for our next lab solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301743145 1. put 4 mL of the MRS broth in ten test tubes 
    2. put 4 mL of the LB broth in ten test tubes 
    3. place caps back on test tubes to make sure they stay sterile 
    4. make the probiotic pill solution (info on precious lab progress column)
    5. add the solutions to the test tubes 
    6. put in incubator
    7. store in warm place 

    ]]>     2018-11-07 19:23:56 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/301743145     We successfully conducted the experiment. taylor_masters9 https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/303511404 We followed all of the steps in the procedures, and left the tubes in the 37 degree incubator. ]]> 2018-11-12 23:23:49 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/303511404 solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/303512078 solutions under the flame ]]> 2018-11-12 23:27:54 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/303512078 test tubes w labels solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/303512164  ]]> 2018-11-12 23:28:15 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/303512164 solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/303512213 l bacillus ]]> 2018-11-12 23:28:34 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/303512213 Today we measured the test tubes in the spectrophotometer solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304375206 2018-11-14 16:10:32 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304375206 solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304376608 2018-11-14 16:13:00 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304376608 L. bacillus test tubes solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304383706 2018-11-14 16:22:50 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304383706 E. coli test tubes solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304384968 Just by looking at these pictures, it is easy to see the difference in clarity in the tubes before we even used the spectrophotometer. ]]> 2018-11-14 16:24:33 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304384968 L. bacillus control solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304386364 2018-11-14 16:26:30 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304386364 L. bacillus water solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304386575 2018-11-14 16:26:49 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304386575 L. bacillus kombucha solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304386825 2018-11-14 16:27:10 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304386825 L. bacillus apple cider vinegar solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304387882 2018-11-14 16:28:39 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304387882 L. bacillus probiotic pill solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304388217 2018-11-14 16:29:07 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304388217 E. coli control solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304391047 2018-11-14 16:33:03 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304391047 E. coli water solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304391176 2018-11-14 16:33:13 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304391176 E. coli kombucha solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304391220 2018-11-14 16:33:19 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304391220 E. coli apple cider vinegar solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304391306 2018-11-14 16:33:25 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304391306 E. coli probiotic pill solene_bossart https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304391415 2018-11-14 16:33:37 UTC https://padlet.com/taylor_masters9/hjpc1x26inx9/wish/304391415