GROUP ACTIVITY: RECOMBINANT TECHNOLOGY by SARAH KALTHOM BINTI RIZA AMARALLAH BEG Moe https://padlet.com/m3811276/h7x5vfdwyqvsu3z2 Group 2 Lisa Pei Yong Sarah Danial en-us 2021-11-05 08:16:15 UTC 2021-11-07 15:54:49 UTC hello@padlet.com Question 4 m3811276 https://padlet.com/m3811276/h7x5vfdwyqvsu3z2/wish/1869465899 a) Transformed bacterial cell will stay alive. Transformed bacteria cell with recombinant plasmid able to grow and amplify itself while the transformed bacteria cell with no plasmid dies.

b) It will die.]]> 2021-11-05 08:19:10 UTC https://padlet.com/m3811276/h7x5vfdwyqvsu3z2/wish/1869465899 Question 3 m3811276 https://padlet.com/m3811276/h7x5vfdwyqvsu3z2/wish/1869466036 a) Mode of action of EcoRl is sticky ends while the mode of action of Smal is blunt ends. EcoRl cuts both DNA strands between base G and base A while Smal cuts both DNA strands between base C and base G.

b) i. Sticky ends are staggered cut single-stranded ends that cleaved by restriction enzyme EcoRl.
     ii. 5'GAATTC3'
        3'CTTAAG5'
     iii. 3

]]> 2021-11-05 08:19:17 UTC https://padlet.com/m3811276/h7x5vfdwyqvsu3z2/wish/1869466036 Question 2 m3811276 https://padlet.com/m3811276/h7x5vfdwyqvsu3z2/wish/1869466205 (a) Q

(b)  - Have selectable genetic markers which are ampicillin resistant gene (ampR) and lacZ
       - Able to accept foreign DNA in multiple cloning sites
       - Able to replicate freely in the host cell.

(c) Recombinant plasmid

(d) Host cell

(e)  - Able to receive recombinant plasmid through the    transformation process.
       - Able to maintain the structure of the recombinant plasmid from one generation to another.
       - Able to amplify the recombinant DNA.

(f) No because the target gene and vector must be cleaved with the same restriction enzyme to produce compatible sticky ends on both the foreign DNA fragments and the plasmid.
]]> 2021-11-05 08:19:25 UTC https://padlet.com/m3811276/h7x5vfdwyqvsu3z2/wish/1869466205 Question 1 m3811276 https://padlet.com/m3811276/h7x5vfdwyqvsu3z2/wish/1869466434 a)  The technology of recombining DNA molecules in vitro by  cutting up DNA molecules and splicing fragments together by different organisms.

b) Source: Bacterial cell
     Role: DNA Cloning vector

c) An enzyme which recognizes and cleave DNA molecule at restriction site.

d) Restriction site: GGATCC
     Restriction enzyme: BamHI

e) i) 2
    ii) 4

f) DNA ligase

g) Bacteria can reproduce fast.]]> 2021-11-05 08:19:36 UTC https://padlet.com/m3811276/h7x5vfdwyqvsu3z2/wish/1869466434