Immunity by Foong May Yeong https://padlet.com/fyeong/immunity class discussion en-us 2016-10-12 00:43:42 UTC 2025-12-09 18:16:55 UTC hello@padlet.com Hypothesis tested fyeong https://padlet.com/fyeong/immunity/wish/130001557 2016-10-12 00:45:11 UTC https://padlet.com/fyeong/immunity/wish/130001557 Materials and Methods fyeong https://padlet.com/fyeong/immunity/wish/130001571 2016-10-12 00:45:17 UTC https://padlet.com/fyeong/immunity/wish/130001571 Results fyeong https://padlet.com/fyeong/immunity/wish/130001581 2016-10-12 00:45:21 UTC https://padlet.com/fyeong/immunity/wish/130001581 Conclusions fyeong https://padlet.com/fyeong/immunity/wish/130001591 2016-10-12 00:45:31 UTC https://padlet.com/fyeong/immunity/wish/130001591 Critique fyeong https://padlet.com/fyeong/immunity/wish/130001621 2016-10-12 00:45:50 UTC https://padlet.com/fyeong/immunity/wish/130001621 Hypothesis https://padlet.com/fyeong/immunity/wish/130319007 immature DC might phagocytose Candida through MFR and subsequently kill the pathogen and process the antigen and stimulate lymphocytes prolieration]]> 2016-10-13 02:58:34 UTC https://padlet.com/fyeong/immunity/wish/130319007 Results https://padlet.com/fyeong/immunity/wish/130319216 1. Equal efficiency in killing (MO& DCs) 
-phagocytotic index: DC phagocytose earlier onset (10mins) as compare to MO (20mins) 
-efficiency by percentage ingestion: DC higher (60%) as compare to MO
2. No difference for opsonised and non-opsonised candida for both cell types 
3. Inhibition of binding of candida on blocking MFR with mannose, fucose & BSA, suggest binding is mediated by MFR, for both cell types 
4. Recognition of candida stimulates both cell type to produce SO
5. Killing of candida is independent of phagocytosis, as demonstrated by incubating both cell types with CD
6. ]]> 2016-10-13 03:01:15 UTC https://padlet.com/fyeong/immunity/wish/130319216 Lymphocyte proliferation https://padlet.com/fyeong/immunity/wish/130320642 Incubate DC with HK or viable candida -> Culture with CD3 T cells -Measure incorporation of [3H]thymine]]> 2016-10-13 03:17:25 UTC https://padlet.com/fyeong/immunity/wish/130320642 Quantify superoxide anion production https://padlet.com/fyeong/immunity/wish/130320928 Quantified by SOD-inhibitable reduction of ferricytochrome c]]> 2016-10-13 03:19:46 UTC https://padlet.com/fyeong/immunity/wish/130320928 https://padlet.com/fyeong/immunity/wish/130321679 - Both DC and macrophage can kill Candida under aerobic and anaerobic conditions.
- C. albicans-infected DC can stimulate T-cell proliferation.]]> 2016-10-13 03:29:10 UTC https://padlet.com/fyeong/immunity/wish/130321679 Phagocytosis assay https://padlet.com/fyeong/immunity/wish/130321799 Ca cells opsonized in PHS]]> 2016-10-13 03:30:44 UTC https://padlet.com/fyeong/immunity/wish/130321799 https://padlet.com/fyeong/immunity/wish/130321909 Candida killing by Macrophages and DCs are oxygen independent]]> 2016-10-13 03:31:57 UTC https://padlet.com/fyeong/immunity/wish/130321909 Results https://padlet.com/fyeong/immunity/wish/130940686 Heat-inactivation process may destroy important immunogenic antigens.]]> 2016-10-15 23:04:48 UTC https://padlet.com/fyeong/immunity/wish/130940686