STRAWBERRY DNA EXTRACTION LAB by Leopoldo Alvarado https://padlet.com/leopoldoa101019/gw81015dbe2s99sr en-us 2022-03-16 15:40:16 UTC 2022-03-27 01:08:21 UTC hello@padlet.com Section 1 leopoldoa101019 https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2098407391 Leopoldo Alvarado

Period: #2

March 1, 2022]]> 2022-03-16 16:28:26 UTC https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2098407391 Section 4 leopoldoa101019 https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2098458630 Materials Used:

-  Heavy duty ziplock bag
- 1 strawberry
- Googles
- 10 mL DNA extraction buffer (soapy, salty water)
- Cheesecloth
- Funnel
- 20 mL test tube & test tube stand
- Stir stick
- 10 mL ethanol (FREEZER)
- Paper towels




]]> 2022-03-16 16:56:03 UTC https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2098458630 Section 2 leopoldoa101019 https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2102563276 DNA stands for Deoxyribonucleic Acid. DNA is made up of adenine, guanine, cytosine, and thymine. Nucleotides are made up of adenine, adenine, guanine, thymine, or cytosine in DNA, and in RNA adenine, guanine, uracil, or cytosine. The base pair rule of DNA would be that adenine always pairs up with thymine and cytosine always pares up with guanine. The nitrogen bases are attached to C1' of deoxyribose through a glycosidic bond. DNA looks like a double helix that looks like ladder that has been twisted.]]> 2022-03-18 22:17:35 UTC https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2102563276 Section 3 leopoldoa101019 https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2102568276 2022-03-18 22:27:51 UTC https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2102568276 Section 5 leopoldoa101019 https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2102569887 1. Get all materials that are needed ]]> 2022-03-18 22:31:35 UTC https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2102569887 Section 5 leopoldoa101019 https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2114451458 2. Smash straw in bag 
3. Add DNA extraction buffer and smash it for another while.]]> 2022-03-25 21:04:45 UTC https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2114451458 Section 5 leopoldoa101019 https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2114467349 4. Assemble your filtration gear as shown in the diagram.]]> 2022-03-25 21:28:18 UTC https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2114467349 Section 5 leopoldoa101019 https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2114470308 5. After assembling your filtration pour the smashed strawberry in the filtration.]]> 2022-03-25 21:33:13 UTC https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2114470308 Section 5 leopoldoa101019 https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2114475450 6. After filtering it and getting as much liquid out pour it into the other test tube]]> 2022-03-25 21:41:24 UTC https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2114475450 Section 5 leopoldoa101019 https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2114478409 7. Then you pour the ethanol and stick in your string stick and take it out with the strawberry DNA already attached to it.]]> 2022-03-25 21:45:36 UTC https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2114478409 Section 6 leopoldoa101019 https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2114482016 First we put the strawberry inside the bag and smash it as much a you can. After smashing it for sometime we added the DNA extraction and smashed it for another while. Then we assembled the our filter and poured in the strawberry trying to get as much liquid through the cheesecloth. After getting as much liquid through the cheesecloth we put it in smaller test tube and then added ethanol to filter strawberry to get the DNA. Then we put in a string stick and pulled it out and it had all the strawberry's DNA sticked on it.]]> 2022-03-25 21:51:59 UTC https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2114482016 Section 7 leopoldoa101019 https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2114502788 What I learned about DNA was that when there is a large amount of it in one spot it will be visible to the human eye. Something that I would do different if I'd do this again would be probably use more strawberries so that there is more DNA and so that it's larger. I guess that the next thing that I would do would be to check if there is DNA in other foods and experiment with other foods. Two traits expressed in a strawberry would be size and sweetnees. ]]> 2022-03-25 22:30:54 UTC https://padlet.com/leopoldoa101019/gw81015dbe2s99sr/wish/2114502788