Group 1 by https://padlet.com/wall/grmvrynkr5 en-us 2014-04-14 04:31:44 UTC 2014-04-14 04:33:57 UTC hello@padlet.com Getting required gene https://padlet.com/wall/grmvrynkr5/wish/25932623 -Break open the cells

-Purify the DNA using phenol and enzymes to remove proteins

-Use restriction enzymes to cut out the required gene

]]> 2014-04-14 04:39:05 UTC https://padlet.com/wall/grmvrynkr5/wish/25932623 Insert it into a vector https://padlet.com/wall/grmvrynkr5/wish/25933211 -Cut the plasmid with the same restriction enzyme used with the gene

-Use DNA ligase to join the gene and plasmid together

]]> 2014-04-14 04:48:17 UTC https://padlet.com/wall/grmvrynkr5/wish/25933211 Put recombinant plasmid into host cells https://padlet.com/wall/grmvrynkr5/wish/25933408 -Electroporation, bacteriophage, gene gun or liposomes

]]> 2014-04-14 04:50:17 UTC https://padlet.com/wall/grmvrynkr5/wish/25933408 Select cells with recombinant vector https://padlet.com/wall/grmvrynkr5/wish/25933534 -Selectable marker a gene that provides the transformants with a new characteristic

that is not possessed by a non-transformant, carried in the plasmid (eg. antibiotic resistance)

-Insertional inactivation distinguishes between transfomants that contain self ligated plasmid from

those that contain recombinant plasmid

]]> 2014-04-14 04:51:55 UTC https://padlet.com/wall/grmvrynkr5/wish/25933534 Cindy Heng, Matilda Woo, Gabriel Leong, Ikmal, Crystal Chee, Shijie https://padlet.com/wall/grmvrynkr5/wish/25933688 2014-04-14 04:53:43 UTC https://padlet.com/wall/grmvrynkr5/wish/25933688