CSI LAB: Dna finger-printing--Marchetti Elena by ElenaM98 https://padlet.com/ele9_marchetti/geb06709a4mt Realizzato con difficoltà en-us 2016-11-29 14:58:16 UTC 2026-03-17 01:28:26 UTC hello@padlet.com https://padlet-assets.s3.amazonaws.com/icons/Soccerball.png Elena Marchetti ele9_marchetti https://padlet.com/ele9_marchetti/geb06709a4mt/wish/140527129 Was it possible to identify people in the past? How?
Yes it was. in the past criminals were identifed by his face, blood group,teeth impression and after 1901 also by finger-prints.
No, it was only possible to exclude suspects. Finger printing  could identity people since 1901.
When was used first DNA finger printing?
Dna finger-printing was  developed in 1984 by an english geneticist called alec J jeffey. Alec J. Jeffreys. DNA fingerprinting is used for first time as an identification technique OF A GHANA' CHILD in 1985. FIRST TEST For a criminal conviction, Based on DNA evidence in USA IS Introduced in 1988.

Where can DNA be obtained from?
DNA can be found in white blood cell, urine, fecis, feces, semen, vaginal fluids ,skin dandruff,hair follicle cells, nasal mucuose mucouse and sweat stain.( blah)

Who invented RFLP technique? When?
In the 70s, the discovery of restriction enzymes,
Bacterial transformation and gel electrophoresis
agarose has initiated the development of  genetic engineering.
1978 Nobel laureate W. Arber, Nathan D. &
H. O'Smith, for the discovery of enzymes
restriction, created found out  the restriction fragment length polymorphism or RFLP.
What is a restriction enzyme?
. In RFLP analysis , DNA is broken into pieces by restriction enzime,  at certain point, specific fot  for any enzime.
because of every man has a little different to each other the lenght of pieces is different.

What is restriction enzyme role in nature?
Restriction enzymes have been developed inside bacteria to prevent viral infection.they cuton pieces duoble stand viral DNA.
]]> 2016-11-29 15:20:00 UTC https://padlet.com/ele9_marchetti/geb06709a4mt/wish/140527129 ele9_marchetti https://padlet.com/ele9_marchetti/geb06709a4mt/wish/141609528 What is the basic principle of gel electrophoresis?
the basic principle of gel electrophoresis is his structure like a net which can separate the pieces of dna whith different lenght.

Why does DNA migrate toward positive electrode?
because  it has a negative charhe for gro many phospate

What is the TBE composition?
It's a solution of Tris-borate-EDTA (ethylenediaminetetraacetic acid). The TBE buffer is particularly useful in the separation of small DNA fragments, like the resulting from the digestion of restriction enzymes.(preparation of agarose electrophoretic gels)TBE has A good buffering capacity (pH 8,8) and provides an accurate resolution.
Why is important to add Mg ions? Are they co-enzymes or cofactors?
mg 2+ are very important because they starded the reaction,; they are inorgarnic molecules so are cofactors.

How can DNA be detected in agarose gel?

DNA be detected in agarose gel with two specific dyes, one long and slow, the other short and fast


]]> 2016-12-04 17:45:00 UTC https://padlet.com/ele9_marchetti/geb06709a4mt/wish/141609528 ele9_marchetti https://padlet.com/ele9_marchetti/geb06709a4mt/wish/141609576 Why are DNA dying dangerous for human health?Dying dna can be cacerogenic causo of dyer  and create muation if it makes contact whit normal cells, assorbed by the skin and immediatly  breated.
What should be done to prepare an agarose gel?  https://www.youtube.com/watch?v=wXiiTW3pflM
you should unit togheter agarose ( variable 0.6 to 3%) and TBE. set the cap on the jar with the solution and move on to the microwave.Be careful at high temperatures and wait time for the dissolution of t agarose. Use always different gloves. Finally put the gel in eletrophoresis support  whit the mask for wells. eliminate bubbles. wait 20 minutes and gel is finished
What should not be done preparing an agarose gel? close the tap., use the same gloves or move the gel while is solidifing.
]]> 2016-12-04 17:45:44 UTC https://padlet.com/ele9_marchetti/geb06709a4mt/wish/141609576 What role does DNA evidence play in solving crimes? ele9_marchetti https://padlet.com/ele9_marchetti/geb06709a4mt/wish/142098425 14"  Why has DNA received a  lot of media attention?
Because it has a good power of discrimination and it's better than a  eyewitness testimony.
36"   How long has DNA  been used to identify people?
from '85, using different tecniques.
52"   The techniques used nowadays are more sensitive. How much DNA is needed to do a test? from 50 ng to 1 ng.
1'26 "How many cells are needed to get a full profile?
full profile can be found in 3 or 4 cells
1' 46" In what conditions must the exhibit be kept?
to keep in good conditons you can freeze ordry it.
1' 52" How long does DNA last if taken in good conditions?
DNA will last essentially forever.
]]> 2016-12-06 17:50:58 UTC https://padlet.com/ele9_marchetti/geb06709a4mt/wish/142098425 Valeria Fregni vfregniddt https://padlet.com/ele9_marchetti/geb06709a4mt/wish/148859854 I contenuti essenziali sono presenti. Si colgono degli approfondimenti pur nella inesistente cura per le maiuscole, il carattere e lo spelling. Puoi impegnarti di più: la videoscrittura è molto usata nel mondo moderno...! Mancano delle parti del portfolio]]> 2017-01-23 20:11:15 UTC https://padlet.com/ele9_marchetti/geb06709a4mt/wish/148859854