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      <title>TISSUE CULTURE TECHNOLOGY by Suhana Binti Zakaria</title>
      <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak</link>
      <description>Please state the disadvantages of tissue culture technology. Don&#39;t forget to add your full name and selfie photo too. Thank you.</description>
      <language>en-us</language>
      <pubDate>2025-10-05 02:54:00 UTC</pubDate>
      <lastBuildDate>2025-11-10 13:40:29 UTC</lastBuildDate>
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      <item>
         <title>Adeline Anak Cogia (F23A0550)</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3618551178</link>
         <description><![CDATA[<p>The disadvantages of tissue culture technology is the process requires more careful maintenance, especially in controlling changes in temperature, light, and humidity.</p>]]></description>
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         <pubDate>2025-10-05 12:08:16 UTC</pubDate>
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      <item>
         <title>Nur Alyaa Binti Noor Hisham (ayaa) F23a0735</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3618708934</link>
         <description><![CDATA[<p>One of the disadvantages of tissue culture technology is risks of contamination by microbes.</p>]]></description>
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         <pubDate>2025-10-05 15:32:39 UTC</pubDate>
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      <item>
         <title>Amierul Syafiq bin Anafiah (F23B0496)</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3620107259</link>
         <description><![CDATA[<p>One of disadvantages is high cost because it requires expensive equipment, sterile lab conditions, and skilled labour.</p><p><br></p>]]></description>
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         <pubDate>2025-10-06 14:32:49 UTC</pubDate>
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      <item>
         <title>NUR FATIHAH IZZATI BINTI AZHAR F23A0325</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3627468039</link>
         <description><![CDATA[<p>One of the disadvantages of tissue culture technology is it does not exactly suitable for most crops. Some of them are difficult to culture or usually have low rate of succesful in vitro.</p>]]></description>
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         <pubDate>2025-10-11 09:25:07 UTC</pubDate>
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      <item>
         <title>Nur Ghina Hijanah Binti Md Faizal (F23B0473)</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3627931125</link>
         <description><![CDATA[]]></description>
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         <pubDate>2025-10-12 04:08:35 UTC</pubDate>
         <guid>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3627931125</guid>
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         <title>AINA AYUNI BINTI ZULKIFLI (F23A0590)</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3627935079</link>
         <description><![CDATA[<p>Disadvantage of the tissue culture is limited to certain species. There are some plant species not respond well to tissue culture methods because some of them are difficult to propagate.</p>]]></description>
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         <pubDate>2025-10-12 04:21:33 UTC</pubDate>
         <guid>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3627935079</guid>
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      <item>
         <title>SITI NURFARAHIN AISYA BINTI MOHD AZNAN (F23A0600)</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3627942642</link>
         <description><![CDATA[<p>Disadvantages of tissue culture is the high risk of contamination if the working area is not kept hygienic.</p>]]></description>
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         <pubDate>2025-10-12 04:45:29 UTC</pubDate>
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         <title>NURUL WAFA BINTI ABDUL MALIK (F23A0702)</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3627943769</link>
         <description><![CDATA[<p>Disadvantages of tissue culture it can make environmental impact for example is energy consumption. Tissue culture requires controlled environments growth chambers, autoclaves, laminar flow hoods that run constantly. But it's can be high energy usage contributes to increased carbon emissions, especially if powered by fossil fuels.</p>]]></description>
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         <pubDate>2025-10-12 04:48:29 UTC</pubDate>
         <guid>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3627943769</guid>
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      <item>
         <title>HANIF ZULFIKRI BIN BAHARUDDIN (F23B0416)</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3627991641</link>
         <description><![CDATA[<p>Require personal skill. Need to train and experience to handle the procedures correctly and maintain sterility.</p><p><br></p>]]></description>
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         <pubDate>2025-10-12 07:05:06 UTC</pubDate>
         <guid>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3627991641</guid>
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         <title>NUR AINA ARISSA BINTI AHMAD BADRUL AMIN (F23A0785)</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3628074728</link>
         <description><![CDATA[<p>Disadvantage of plant tissue culture are easy to get contaminated from fungi and bacteria if not proper sterile technique.</p>]]></description>
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         <pubDate>2025-10-12 09:49:18 UTC</pubDate>
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      <item>
         <title>ELISA YASMIN BINTI SHAHRULNIZAM (F23A0365)</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3628309853</link>
         <description><![CDATA[<p><br/></p><p>The disadvantage of producing plants through tissue culture is the potential for unwanted genetic changes, known as <em>somaclonal variation</em>. It could have occurred due to stress, prolonged culture time, or hormone imbalance during the process.<br></p>]]></description>
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         <pubDate>2025-10-12 14:45:16 UTC</pubDate>
         <guid>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3628309853</guid>
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      <item>
         <title>ADDILIN JENIOL, F23A0790</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3629280249</link>
         <description><![CDATA[<p>Disadvantages of tissue culture are its is expensive, easy contaminated, is not suitable for all plants and depends on controlled.</p>]]></description>
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         <pubDate>2025-10-13 07:10:55 UTC</pubDate>
         <guid>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3629280249</guid>
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      <item>
         <title>F23B0835_Syafiqah Nabilla Chek Ruzuman</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3629927858</link>
         <description><![CDATA[<p>Tissue culture needs a super clean lab, takes a lot of careful work, and if one plant gets infected, the whole batch can be ruined.</p>]]></description>
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         <pubDate>2025-10-13 15:05:59 UTC</pubDate>
         <guid>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3629927858</guid>
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         <title>NUR AISHAH BINTI MOHD ROSLI F23A0566</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3639029503</link>
         <description><![CDATA[<p>The disadvantage of tissue culture technology are challenges of acclimatization. When transplanted from the lab to the field, in vitro-grown plantlets frequently struggle to adjust to their natural surroundings. High mortality rates are often the result of this period, which is called acclimation.</p>]]></description>
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         <pubDate>2025-10-19 02:15:56 UTC</pubDate>
         <guid>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3639029503</guid>
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         <title>NUR AFIRAH BINTI SALMI F23A0779</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3642820824</link>
         <description><![CDATA[<p>Risk of contamination</p><p><br/></p><p>Cultures are easily contaminated by bacteria, fungi, or viruses. Once contamination occurs, the entire batch may be lost.</p><p><br/></p>]]></description>
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         <pubDate>2025-10-21 09:44:25 UTC</pubDate>
         <guid>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3642820824</guid>
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      <item>
         <title>MUHAMMAD AZFAR</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3661313269</link>
         <description><![CDATA[<p>disadvantages: need professional worker to do the job (bukan sembarangan orang boleh buat)</p>]]></description>
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         <pubDate>2025-11-01 14:56:01 UTC</pubDate>
         <guid>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3661313269</guid>
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      <item>
         <title>ARIF ZAKWAN BIN ZAINAL ABIDIN (F23B0825)</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3672998978</link>
         <description><![CDATA[<p>Disadvantages of tissue culture are Economics and Resource Limitation because high in start-up costs. A tissue culture lab is very expensive to set up, it needs specialized machinery, sterile operation, and trained personnel.</p>]]></description>
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         <pubDate>2025-11-08 14:58:39 UTC</pubDate>
         <guid>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3672998978</guid>
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      <item>
         <title>VICTORIA CHOH (F23A0543)</title>
         <author></author>
         <link>https://padlet.com/suhana3/fiq64ft2ncaqb4ak/wish/3675327224</link>
         <description><![CDATA[<p>One of the disadvantage of tissue culture is expensive and requires skilled workers to prevent contamination.</p>]]></description>
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         <pubDate>2025-11-10 13:40:28 UTC</pubDate>
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