Polymerase chain reaction PCR (Molecular genetic testing) by Gilda Reynolds https://padlet.com/greynolds4/f53luthfg54z There are several types of genetic testing, as genetic engineers your job is to explore Molecular genetic testing using PCR. Use your textbook, internet and links provided find out detailed information on molecular genetic testing using PCR and describe how the process is done. Your research should include the following information 1. A detailed explanation on how would you test the gene with an example. 2. Limitations of PCR when used for genetic testing. 3.How is it different from karyotyping or cytogenetic testing. en-us 2017-10-15 21:24:34 UTC 2026-03-09 01:19:56 UTC hello@padlet.com https://padlet.com/greynolds4/f53luthfg54z/wish/197172535 PCR is a technique used for DNA amplification ( make many copies )  of a piece of DNA. ]]> 2017-10-15 22:46:55 UTC https://padlet.com/greynolds4/f53luthfg54z/wish/197172535 The limitations of PCR https://padlet.com/greynolds4/f53luthfg54z/wish/197172807 The main limitation comes when you use PCR as an end-point test. This is because the determining factor always comes in the gel run, matching the size of the expanded fragment against your expectation. The gel run gives you a yes/no answer. It is not quantitative. 
  1. Test inhibition: Samples from complex biological sites (e.g. stool) can contain substances that interfere with the many complex molecular reactions upon which the tests rely. Without good (and proven) methods to prepare the sample, this can result in a false negative result.
2. Sample contamination: This is a common concern with highly sensitive molecular tests – even a minute amount of contamination in the sample can cause a false positive result.
]]> 2017-10-15 22:49:27 UTC https://padlet.com/greynolds4/f53luthfg54z/wish/197172807 The Polymerase Chain Reaction https://padlet.com/greynolds4/f53luthfg54z/wish/197174216 BY MERNA 

]]> 2017-10-15 23:04:54 UTC https://padlet.com/greynolds4/f53luthfg54z/wish/197174216 https://padlet.com/greynolds4/f53luthfg54z/wish/197174268 2017-10-15 23:05:38 UTC https://padlet.com/greynolds4/f53luthfg54z/wish/197174268 greynolds4 https://padlet.com/greynolds4/f53luthfg54z/wish/197650041 http://learn.genetics.utah.edu/content/labs/pcr/

]]> 2017-10-17 05:52:04 UTC https://padlet.com/greynolds4/f53luthfg54z/wish/197650041 greynolds4 https://padlet.com/greynolds4/f53luthfg54z/wish/197650076 https://www.dnalc.org/resources/animations/pcr.html]]> 2017-10-17 05:52:23 UTC https://padlet.com/greynolds4/f53luthfg54z/wish/197650076 Karyotyping https://padlet.com/greynolds4/f53luthfg54z/wish/198053756 Karyotyping is used to detect numerical or structural chromosome abnormalities i metaphase cells. This method i useful in detecting large changes in chromosomes using light microscopic methods.


]]> 2017-10-17 23:41:57 UTC https://padlet.com/greynolds4/f53luthfg54z/wish/198053756 How PCR works https://padlet.com/greynolds4/f53luthfg54z/wish/198054357 PCR is a technique used to make many copies of a piece DNA. 

A DNA sample is mixed with short lengths of DNA, and other chemical in a tube, then the mixture is heated and cooled at intervals. At each cycle of synthesis, the DNA is duplicated, causing a large amount of DNA to be produced. ]]> 2017-10-17 23:46:34 UTC https://padlet.com/greynolds4/f53luthfg54z/wish/198054357 Polymerase chain reaction https://padlet.com/greynolds4/f53luthfg54z/wish/198054600 PCR is a technique used for DNA amplification of a piece of DNA. The amplified DNA is treated with an enzyme to identify specific alleles]]> 2017-10-17 23:48:44 UTC https://padlet.com/greynolds4/f53luthfg54z/wish/198054600