Electric eels by jayen prashanth https://padlet.com/ejayen/f4mp9y78v3i66voj A place to collect all information, in a brief comprehensible manner, about the findings in the electrochemical journey en-us 2021-04-08 17:17:43 UTC 2024-11-03 13:38:35 UTC hello@padlet.com Choice of materials ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420214481 1. Carbon/Graphene 
2. Gold 
3. ITO - Special property - Optically transparent]]> 2021-04-15 09:26:29 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420214481 The linker is a molecule, in which, one part of the molecule binds to the electrode surface and the other part binds to the antibody. ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420216025 Linker Gold - Antibody: https://docs.google.com/presentation/d/1m_GG_4xkAsVrLlTqztFlHAbDm6v7LkK7/edit#slide=id.p1

Linker Carbon - Antibody: TODO ]]> 2021-04-15 09:27:11 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420216025 ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420234384 2021-04-15 09:34:54 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420234384 Cyclic and differential voltammetry ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420238544 https://www.youtube.com/watch?v=em1bBmkj6Xg&t=526s

Cyclic voltammetry - to characterise the electrode.
Differential voltammetery - For quantitative measurements.
Both are done together in the same test.

Quentin: Use a redox probe molecule in the electrochemical solution: Ferro/ferricyanide

Quentin & previous Sensus: The size of the viral particle might be too small to produce a blocking effect at the electrode surface. Quentin suggested that we could do a sandwich ELISA to improve the blocking effect.

]]> 2021-04-15 09:36:39 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420238544 Use of gold nano-particles ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420242060 Quentin: "You can still use gold nanoparticles to improve your signal. They can still be used to improve electron transfer and increase the specific surface area of your electrode (At the nanometric scale the surface / volume ratio is greater, hence the interest of materials at the nanometric scale). They use a similar process in the paper I attached"]]> 2021-04-15 09:38:11 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420242060 Screen printing process ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420245303 https://www.youtube.com/watch?v=EwEe6jqBJ_4

Advantage: Cost effective, small in size, easy to manufacture in bulk.

Abdul:
* Possible to screen-print in the chem lab. 
* For carbon electrodes, we would need carbon paste and then a mask with the electrode pattern. 
* The pattern could be designed on CAD. The pattern could be designed by Abdul on behalf of SensUs.]]> 2021-04-15 09:39:34 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420245303 BSA ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420281499 BSA solution could be used to block the electrode surface from non-specific binding. 

BSA powder is in the lab, confirmed by Ehsan ]]> 2021-04-15 09:54:37 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420281499 ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420309166 Quentin: It is important to prevent non-specific binding on the electrode surface. Otherwise, you might get a false signal. ]]> 2021-04-15 10:06:33 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420309166 ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420332985 2021-04-15 10:17:52 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420332985 ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420337277 Microfluidic devices from Zimmer and Peacock to spark some ideas:

https://www.youtube.com/watch?v=sIW-fQPsyyQ&t=19s

https://www.youtube.com/watch?v=ZwOsOZQF4N0&t=7s

]]> 2021-04-15 10:19:47 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420337277 ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420348162 2021-04-15 10:24:48 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420348162 Electrochemical impedance spectroscopy ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420353600 https://www.youtube.com/watch?v=DPnu8BdPL3w&t=1305s

All the Skeptics: Too sensitive, non-reproducibility of results.

Zhen Zhang: The environmental conditions might affect the electrochemical signal. try to isolate the measurement process. ]]> 2021-04-15 10:27:13 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420353600 Electrodes we have now ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420369259 * 14 electrodes from PalmSens
Luis: the working electrode should be gold, the reference should be silver/silver chloride. I think the counter electrode was also gold, or it may have been platinum, but I think it was gold.

* 3 gold electrodes from Quentin ]]> 2021-04-15 10:34:39 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420369259 ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420405235 2021-04-15 10:51:23 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420405235 Hardware: Potentiostat from PalmSens ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420408069 https://www.palmsens.com/product/oem-emstat-pico-development-kit/]]> 2021-04-15 10:52:46 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420408069 ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420410595 2021-04-15 10:53:58 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420410595 ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420411566 2021-04-15 10:54:28 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420411566 ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420411852 2021-04-15 10:54:38 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420411852 Software: PsTrace from PalmSens ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420413899 https://www.palmsens.com/software/ps-trace/]]> 2021-04-15 10:55:39 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420413899 ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420416665 2021-04-15 10:57:02 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420416665 Mobile Application: ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420418264 How do you want the application to look like. Add abstract ideas here.]]> 2021-04-15 10:57:52 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420418264 https://www.zimmerpeacocktech.com/2021/03/27/free-questions-and-answers-with-zp/ ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420459423
Forum for the electrochemical process. Responses are quick and discussed in detail on every Thursday webinar at 8AM UK time. 

https://www.dev.zimmerpeacock.com/members-webinars]]> 2021-04-15 11:17:34 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420459423 Add questions here ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420467645 2021-04-15 11:21:09 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420467645 Preparation of ITO electrodes ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420618407 Sofia: I currently use ITO deposited on glass by a company(Visiontek Systems Ltd visiontek.sales@gmail.com). This to be certain to always get the same sheet resistance. I have used HCl etching to pattern the ITO, but now I use the laser engraver in the actuator lab, this results in some surface roughness on the etched parts, but OK for my purposes (TEER measurements). In the clean room, it is possible to sputter ITO on glass and possibly also on polymer substrates. Even though it is sputtering, the temperature goes up during the deposition process to above 90C (I am not sure how much). If the polymer is too soft, the conductor would crack easily  - this could be the case for PDMS. If you use the in-house sputtering (available to myfab users), thin films of ITO could also be patterned by lift-off.
You could also talk to the solar cell group (eg Tomas Kubart) as they are experts on depositing TCO:s and have even more deposition tools (but they never pattern their films as far as I know).
]]> 2021-04-15 12:16:57 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1420618407 Linkers! https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1429405442 - If we need to bind the antibodies to gold we'll use "4-mercaptobenzohydrazide"
- We'll have access to a lab in BMC (organic chemistry) to synthesize the linker.  ]]> 2021-04-18 08:03:39 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1429405442 Nyquist plot ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1460329452 https://www.youtube.com/watch?v=9j7PMgFmAq0

]]> 2021-04-26 16:12:09 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1460329452 Cause of phenomenon ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1464574876 https://reader.elsevier.com/reader/sd/pii/S0013468616307381?token=19D09E6A4037F01D021E0FD3EFB9583316425A24B18935D7961963CB5C5568ADE85806E00383C7076B2FD32E0C9A996D&originRegion=eu-west-1&originCreation=20210427133448]]> 2021-04-27 14:31:12 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1464574876 Bode Plot ejayen https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1468812247 https://www.youtube.com/watch?v=yn9ypACptGI]]> 2021-04-28 12:53:02 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1468812247 Saturday - May 1 caarce93 https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1483120852 We ran experiments on DI water, identified used and new electrodes. ]]> 2021-05-02 21:15:54 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1483120852 Saturday - May 1 caarce93 https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1483121805 -We ran experiments on DI water, the signal obtained for Impedance is very unstable and non reproducible even using the same electrode and the same amount of solution. 

-We need to test it with the electrochemical probe.]]> 2021-05-02 21:16:28 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1483121805 May 19 caarce93 https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1540924741 First attempt to synthesize the hydrazide linker.
Reaction gave a mixture of products, optimization needed.
]]> 2021-05-19 18:31:56 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1540924741 Electrodes from Z & P caarce93 https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1588240172 Arrived!]]> 2021-06-06 19:03:22 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1588240172 June 6 caarce93 https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1588240365 I ]]> 2021-06-06 19:03:35 UTC https://padlet.com/ejayen/f4mp9y78v3i66voj/wish/1588240365