Bio 376 Winter 2024 by Higginbotham, Holden https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb Totally cellular en-us 2022-04-09 20:00:32 UTC 2024-03-01 00:48:35 UTC hello@padlet.com https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2843325091 With the enzymes allowing the activation energy for a reaction to require less energy does that mean there is more free energy left over?

]]> 2024-01-09 19:52:08 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2843325091 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2843439707 How are the change in Free Energy and the change in standard free energy different? Delta G vs Delta G w/ degree symbol?

]]> 2024-01-09 22:16:17 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2843439707 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2843579230 Do covalent bond contribute the same way to the shape, function and interaction of molecules as non-covalent bonds?

]]> 2024-01-10 01:46:57 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2843579230 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2843746530 Coupling of reactions

]]> 2024-01-10 05:08:29 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2843746530 Molecular motions https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2844415487 2024-01-10 16:02:39 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2844415487 Co-immunoprecipitation https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2846321541 I don't get how it helps us understand the function on a protein.

]]> 2024-01-12 01:18:20 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2846321541 Cell Culture https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2846443233 What are the requirements for a successful cell culture? The book mentions suspension not being the best way to grow culture, but does that mean eukaryotic cell cultures shouldn't have any liquid on them?

]]> 2024-01-12 04:00:57 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2846443233 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2846454451 what is a purified cell-free system? and how does this methodogical approach align with the reductionist approach?


]]> 2024-01-12 04:18:55 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2846454451 In vivo vs. primary culture https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2846505003 Is in vivo only experiments on living organisms? Or does it relate to primary culture, since it's directly from the organism's tissue?

]]> 2024-01-12 05:43:06 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2846505003 SDS-PAGE and Western Blot https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2846518677 I would like a better explanation of how these two work.


]]> 2024-01-12 06:05:41 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2846518677 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2854725815 What is the difference between polypeptide chains and nascent polypeptide chains?

]]> 2024-01-19 13:56:23 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2854725815 PrP* and Free-energy https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2859174740 In terms of free-energy, why does PrP* pull normal PrP into an aggregate form but, when it is initially converting, it is hard to turn PrP into PrP*?

]]> 2024-01-23 19:40:40 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2859174740 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2859358302 What is the proteolytic machinery?


]]> 2024-01-23 23:28:00 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2859358302 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2859372616 how do the degradation system recognizes unfolded or misfolded proteins?

]]> 2024-01-23 23:54:42 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2859372616 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2860235629 What's the N-end rule?


]]> 2024-01-24 14:30:23 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2860235629 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2860330875 An overview of the processes of E1, E2, and E3

]]> 2024-01-24 15:34:56 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2860330875 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2865296693                                                                

If the final folded shape of a protein is the conformation or lowest free energy then how can a protein’s shape be changed by allosteric regulation? Wouldn’t changing shape be an energetically unfavorable reaction?

]]> 2024-01-29 14:15:25 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2865296693 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2865329491                                                                    What are the two ways protein phosphorylation can affect the activity of the protein?

]]> 2024-01-29 14:35:28 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2865329491 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2865330013                                                               i.      There is a protein with an SH2 domain that binds to another protein with a phosphorylated tyrosine. However, this protein with the SH2 domain doesn’t just bind to any old protein with a phosphorylated tyrosine. It’s choosy about which ones it binds to. why?

]]> 2024-01-29 14:35:49 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2865330013 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2865332005 What proportion of proteins in the cell is thought to be phosphorylated at any given time?

]]> 2024-01-29 14:37:04 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2865332005 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2865332671 A single protein can be phosphorylated in more than one place at the same time?


]]> 2024-01-29 14:37:28 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2865332671 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2865372149 What's induced proximity?

]]> 2024-01-29 15:01:36 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2865372149 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2870930399 What's a micelle?

]]> 2024-02-02 07:29:15 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2870930399 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2876688635 In a multi pass transmembrane protein how does the final destination (golgi or plasma membrane) impact where the loops will be?

]]> 2024-02-07 14:47:39 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2876688635 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2891011329 How to find half life in equilibrium

]]> 2024-02-21 14:26:06 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2891011329 https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2901345637 Could you go over MAP kinase signaling and Ras GTPases

]]> 2024-03-01 00:48:35 UTC https://padlet.com/higginbothamh/f2nmnkrlk1dq7irb/wish/2901345637