Antifungal by Foong May Yeong https://padlet.com/fyeong/antifungals Discsussion en-us 2016-10-15 07:49:01 UTC 2016-10-17 03:28:00 UTC hello@padlet.com Hypothesis fyeong https://padlet.com/fyeong/antifungals/wish/130899906 2016-10-15 07:55:44 UTC https://padlet.com/fyeong/antifungals/wish/130899906 Methods fyeong https://padlet.com/fyeong/antifungals/wish/130899909 2016-10-15 07:55:48 UTC https://padlet.com/fyeong/antifungals/wish/130899909 Results fyeong https://padlet.com/fyeong/antifungals/wish/130899911 2016-10-15 07:55:52 UTC https://padlet.com/fyeong/antifungals/wish/130899911 Conclusions fyeong https://padlet.com/fyeong/antifungals/wish/130899914 2016-10-15 07:55:57 UTC https://padlet.com/fyeong/antifungals/wish/130899914 Critique fyeong https://padlet.com/fyeong/antifungals/wish/130899917 2016-10-15 07:56:02 UTC https://padlet.com/fyeong/antifungals/wish/130899917 hypothesis https://padlet.com/fyeong/antifungals/wish/131027176 a defect in DNA repair may account for accelerated emergence of various genetic changes responsible for drug resistance, in C. glabrata]]> 2016-10-17 03:09:28 UTC https://padlet.com/fyeong/antifungals/wish/131027176 results https://padlet.com/fyeong/antifungals/wish/131027337 MSH2 deletion leads to an increase in resistant mutant

msh2 mutations identified in diverse clinical isolates 

colonization with msh2 mutant leads to increased resistance in vivo (mice model)

WT cells partially outcompete msh2 mutant in mixed inoculation mouse model]]> 2016-10-17 03:11:37 UTC https://padlet.com/fyeong/antifungals/wish/131027337 methods https://padlet.com/fyeong/antifungals/wish/131027944 gene disruption and cloning 

drug susceptibility assay- broth micro- dilution  & CFU count 

mice model: immunocompetent mice- treated with antibiotics (PTZ) & challenged with WT or msh2 mutant, fungal burden was determined by plating and & CFU count. 

fitness assay: mice model immunocompetent and immunosuppressed- use qPCR to identify strains

]]> 2016-10-17 03:19:25 UTC https://padlet.com/fyeong/antifungals/wish/131027944 https://padlet.com/fyeong/antifungals/wish/131028192 High percentage of clinical C. glabrata isolates carry loss-of-function mutations in the MMR gene MSH2, resulting in production of mutator phenotype that is resistant to many anti-fungals
- MSH2 can be potentially used as a biomarker throughout course of therapy to determine likelihood of the pathogen generating drug-resistant mutants]]> 2016-10-17 03:22:02 UTC https://padlet.com/fyeong/antifungals/wish/131028192