DNA Replication by SreenivasanT-Teacher -Mathakondapalli Model School https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg Dear Children Solve the worksheet given on DNA replication. en-us 2021-08-02 06:43:51 UTC 2023-03-27 12:28:30 UTC hello@padlet.com Pavithran 1243sreenivasant https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1666526977 1.       Is DNA replication described as conservative or semi-conservative? What does that mean?
DNA replication is semi-conservative because each helix that is created contains one strand from the helix from which it was copied.
2.       Expand DNA
Deoxyribo nucleic acid.
3.       Expand RNA
Ribonucleic acid
4.       What are the 3 parts of a nucleotide?
Nitrogen bases, Pentose sugar and phosphate group
5.       The pyrimidine bases are?
cytosine, thymine, and uracil.
6.       The purine bases are?
Adenine  and Guanine
7.       Is DNA replication a catabolic or anabolic process? Anabolic process
8.       What is the starting point in replication called?
Primer
9.       Which strand is synthesized towards the replication fork?
Leading strand
10.   What type of bond does helicase break?
Hydrogen Bond
11.   What is at the 5’ end of DNA? What about the 3’ end?
5'=5 primer
3'=3 primer
12.   List the enzymes used in DNA Replication?Function?
DNA primase, DNA helicase, DNA ligase, and topoisomerase.
13.   Which of the base pairs would be harder to split? Why?
3 hydrogen bonds hold it together while A-T only is held together by 2 hydrogen bonds.
14. Who proposed semiconservative Model of DNA replication>
Semi-Conservative DNA Replication: Meselson and Stahl. Watson and Crick's discovery of DNA structure in 1953 revealed a possible mechanism for DNA replication.

15. Which of the strands (leading or lagging) would use more RNA primer?Why

The leading strand is copied in that direction so only one primer is needed to “seed” the replication process. But for the lagging strand, the elongation still needs to move along the daughter strand in the same direction.







]]> 2021-08-02 07:56:30 UTC https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1666526977 Nikhil.M.S balamugunthan4591 https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1666591300 1. Because in each helix is created contains one strands from the helix which it was copied.Therefore,DNA replication is semi-conservative. 
2. Deoxy ribonuleic acid 
3. Ribonucleic acid 
4. Phosphate group, nitrogen bases and Pentose sugar are the 3 parts of nucleotide. 
5. Cytosine, thymine and uracil are the parts of pyrimidine bases
6. Adenine and guanine are the parts of purine bases. 
7. DNA replication is an anabolic process. 
8. Primer 
9. Leading strand
10. Hydrogen bonds 
11. In  5' it ends of DNA and in 3' it starts of DNA.
12. Helicase, DNA polymerase, primase and ligase.
13. Cytosine and guanine are harder to split.
14. Matthew meselson and frankin stahl proposed the semiconservative of DNA replication 
15.lagging strand.]]> 2021-08-02 09:37:05 UTC https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1666591300 Sorna harini. D https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670681440 1.DNA replication is semiconservative because each helix that is created contains one strand from the helix from which it was copied.

2.Deoxy ribonucleic acid

3.Ribo nucleic acid

4.Three parts of nucleotide are nitrogen base, Pentose sugar and phosphate group

5.Pyrimidine bases are cytosine,thymine,uracil
                                                                 
6.Purine bases are adenine ,guanine
                               
7.DNA replication is an Anabolic process

8. The starting point of DNA replication is origin

9. Leading strand is synthesized.

10. They break hydrogen bonds.

11.5'end end of DNA strand. 3'end start of DNA strand.

12.Enzymes involved are
I)Helicase
II) DNA polymerase
III) Primer
IV) DNA ligase

13.The guanine -cytosine base pair would be hardest to break because they three hydrogen bond holding them together.
 
14.Mathew and sthal proposed semiconservative model of DNA.

15.Lagging strand   the lagging strand is made in short Okazaki fragments, several RNA primers are necessary in a repeating cycle of primer initiation, elongation by DNA polymerase, and ligation to seal the fragments 

 
]]> 2021-08-06 03:39:45 UTC https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670681440 Vipanchy 01243202001080 https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670682775 1.       Is DNA replication described as conservative or semi-conservative? What does that mean?
DNA replication is a semiconservative  process because when a new double-stranded DNA molecule is formed, one of the two strands will be from the original template molecule

2.       Expand DNA
Deoxyribo nucleic acid.

3.       Expand RNA
Ribonucleic acid

4.       What are the 3 parts of a nucleotide?
 #Nitrogen bases
 #Phosphate group
 #Pentose sugar

5.       The pyrimidine bases are?
cytosine, thymine, and uracil

6.       The purine bases are?
Adenine and Guanine

7.       Is DNA replication a catabolic or anabolic process?
Anabolic process

8.       What is the starting point in replication called?
Primer

9.       Which strand is synthesized towards the replication fork?
Leading strand

10.   What type of bond does helicase break?
hydrogen bonds between the nucleotide base pairs 

11.   What is at the 5’ end of DNA? What about the 3’ end?
5' end_____ end of the DNA strand
3' end_____ start of the DNA strand

12.   List the enzymes used in DNA Replication?Function?
Helicase (unwinds the DNA double helix)
Primase (lays down RNA primers)
DNA polymerase (main DNA synthesis enzyme)
Ligase (fills in the gaps)

13.   Which of the base pairs would be harder to split? Why?
Guanine-Cytocine is harder to break because 3 hydrogen bonds hold it together

14. Who proposed semiconservative Model of DNA replication?
Meselson and Stahl

15. Which of the strands (leading or lagging) would use more RNA primer?Why ?
Because during lagging strand synthesis, the template DNA strand runs in the wrong direction.

DNA polymerase moves in one direction, but has to copy both strands at the same time, and those two strands run in opposite directions.

https://images.app.goo.gl/TaPDXb6kRT4XqTzw6



]]> 2021-08-06 03:41:18 UTC https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670682775 Sharumathi sharurohin https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670684193 1.) DNA replication is semi-conservative because each helix that is created contains one strand from the helix from which it was copied.
2.) Deoxyribonucleic Acid
3.) Ribonucleic Acid
4.) Nitrogen bases, Pentose Sugar, phosphate groups
5.) Cytosine, Thymine, Uracil
6.) Adenine, Guanine
7.) DNA replication is an Anabolic Process
8.) The start of the DNA replication is called Origin
9.) Leading strand
10.) Hydrogen Bonds
11.) 3' = 3 prime
        5' = 5 prime
12.) RNA primer, Helicase, DNA Polymerase, DNA ligase, Topoisomerase
13.)
14.) Matthew Messolson & Franklin Stahl's
15.) The Lagging Strand.  the lagging strand, the direction of syntesis is opposite and the DNA polymerase with its only 5′ to 3′ end activity gets stuck after short time and this lagging strand is synthesized in short Okazaki fragments. And because the polymerase can’t just start where it wants, it needs primers. A lot of them. Extending the primed segments, Okazakiho fragments are made. After removing primers and their replacement with DNA, the segments are joined with DNA ligase which forms the DNA strand




]]> 2021-08-06 03:42:59 UTC https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670684193 Barath.P https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670684396 1) DNA replication is semi-conservative because each helix that is created contains one strand from the helix from which it was copied.
2) Deoxyribo nucleic acid
3) Ribo nucleic acid
4)  1.phosphate group
     2.pentose sugar
     3.nitrogen base
5)  1.cytosine
     2.thymine
     3.uracil
6)  Adenine , Guanine
7) Anabolic process
8) Origin
10) Hydrogen
11) 5' =5 prime is end of dna
     3' =3 prime is start of dna
12) DNA primase
      DNA helicase
      DNA ligase
13) G-C is harder to break because 3          while A-T only is held together by 2
      hydrogen bases
14) Mathew
15) Lagging strand]]> 2021-08-06 03:43:14 UTC https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670684396 GURU PRASAD https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670687507
  • DNA replication is semi-conservative because each helix that is created contains one strand from the helix from which it was copied.
  • Deoxyribonucleic acid (DNA).
  • Ribonucleic acid (RNA).
  • Nitrogen bases,
    Pentose sugar,and
    Phosphate group.
  • Cytosine,
    Thymine,and 
    Uracil.
  • Adenine & Guanine.
  • Anabolic processing.
  • Primer is the starting point in replication.
  • Leading strand is synthesized towards the replication fork.
  • Hydrogen bond.
  • 5'>>5 prime is exit of DNA
     3'>>3 prime is entrance of DNA.
  • DNA primase,
    •       DNA helicase,&
          DNA ligase.
     13. (G)=(C) is harder to breakdown because,3 while (A)=(T) only is held together by 2 hydrogen bases.
    14. Mathew.
    15. Lagging strand.

    ]]>     2021-08-06 03:46:48 UTC https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670687507     Deva baarathi 01243202001096 https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670688284
    1. DNA replication is semi-conservative
    2. Deoxyribonucleic Acid
    3. Ribonucleic Acid
    4. Nitrogen base,
         Pentose sugar,
         Phosphate group.
    5.  Cytosine
         Thymine
         Uracil
    6.  Guanine & Adenine
    7.  It is an Anabolic  Process
    8. Origin
    10. Hydrogen bond
    11. 3'- 3 prime
          5' - 5 prime
    12. RNA primer,
          Helicase,
          DNA polymerase,
               DNA ligas,
               Topoisomerase.
    13.
    14. Franklin stahl's
          & Mathew Messolson.






    ]]>     2021-08-06 03:47:41 UTC https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670688284     POOVARASAN T https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670707360 1) Answer: DNA replication is semi-conservative because each helix that is created contains one strand from the helix from which it was copied.
    2) Deoxyribo nucleic acid.
    3) Ribonucleic acid
    4)     Nitrogen bases, Pentose sugar and phosphate group
    5) pyrimidines are cytosine, thymine, and uracil.
    6) The two purine bases are adenine and guanine
    7) DNA replication is an anabolic process. Metabolism is the sum of all the chemical reactions in the body, which can be divided into two pathways.
    8) DNA replication initiates at specific points, called origins, where the DNA double helix is unwound. A short segment of RNA, called a primer, is then synthesized and acts as a starting point for new DNA synthesis.
    9) leading strand” is synthesized continuously toward the replication fork as helicase unwinds the template double-stranded DNA.
    10) The process of breaking the hydrogen bonds between the nucleotide base pairs in double-stranded DNA requires energy.
    11) One end is referred to as 5' (five prime) and the other end is referred to as 3' (three prime). The 5' and 3' designations refer to the number of carbon atom in a deoxyribose sugar molecule to which a phosphate group bonds.
    12) DNA replication requires other enzymes in addition to DNA polymerase, including DNA primase, DNA helicase, DNA ligase, and topoisomerase.
    13) G-C is harder to break because 3 hydrogen bonds hold it together while A-T only is held together by 2 hydrogen bonds.
    14) Semi-Conservative DNA Replication: Meselson and Stahl. Watson and Crick's discovery of DNA structure in 1953 revealed a possible mechanism for DNA replication.
    15) lagging strand]]>     2021-08-06 04:09:43 UTC https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670707360     Vignesh balamugunthan4591 https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670725295 1.DNA replication is semiconservative because each helix that is created contains one strand from the helix from which it was copied.
    2.Deoxy ribonucleic acid
    3.Ribo nucleic acid
    4.Three parts of nucleotide are nitrogen base, Pentose sugar and phosphate group
    5.Pyrimidine bases are cytosine,thymine,uracil
                                                                     
    6.Purine bases are adenine ,guanine
                                   
    7.DNA replication is an Anabolic process
    8. The starting point of DNA replication is origin
    9. Leading strand is synthesized.
    10. They break hydrogen bonds.
    11. Each end of DNA molecule has a number. One end is referred to as 5' (five prime) and the other end is referred to as 3' (three prime). The 5' and 3' designations refer to the number of carbon atom in a deoxyribose sugar molecule to which a phosphate group bonds.
    12. DNA replication requires other enzymes in addition to DNA polymerase, including DNA primase, DNA helicase, DNA ligase, and topoisomerase.
    13.Which base pairs would be harder to split, A-T or G-C? Why? G-C is harder to break because 3 hydrogen bonds hold it together while A-T only is held together by 2 hydrogen bonds. Which of the strands (leading or lagging) would use more RNA primer?
    14.Semi-Conservative DNA Replication: Meselson and Stahl. Watson and Crick's discovery of DNA structure in 1953 revealed a possible mechanism for DNA replication.
    15.The DNA polymerase also needs a RNA primer on the leading strand to be able to start polymerization. Afterwards this is not needed anymore, since the replication goes on without a break. On the lagging strand polymerization replication can only work between the replication fork and the next region of double-stranded DNA. See the figure (from here):

    ]]>     2021-08-06 04:31:17 UTC https://padlet.com/1243sreenivasant/eylgsn5cq2mf8zrg/wish/1670725295