Room #2 May 10-12th ASK BIO Workshop Breakout Room Template by ASK BIO team https://padlet.com/askbio/dwg63hmwlnsctey7 Summer 2022 en-us 2022-05-10 13:29:33 UTC 2024-10-27 16:17:53 UTC hello@padlet.com Adding a new post askbio https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2177601056 Click on the (+) symbol. 
You can copy your text in the box and even add images. (use the up arrow below to add images to your question]]> 2022-05-10 13:29:33 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2177601056 askbio https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2177601057 2022-05-10 13:29:33 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2177601057 Homework askbio https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2177601058
  • Consider some of your own test items.  
  • Use the item writing guidelines and work to improve TWO of your items. 
  • Under your name (Q1 & Q2), post your original question and then copy and paste the revised question from Google Docs.
  • The Feedback column will be empty.

    • ]]>     2022-05-10 13:29:33 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2177601058     Original Question askbio https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2177601059 2022-05-10 13:29:33 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2177601059 Revised Question askbio https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2177601060 2022-05-10 13:29:33 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2177601060 You found a lizzard with a cut tail. You pick it up at home to help it recover. After some days you realize the tail is fine. Among all processes, what is the process by which tail regenerated? https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2179965161 2022-05-11 18:52:23 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2179965161 Which macromolecules are part of the central dogma in biology https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2179966728 2022-05-11 18:53:13 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2179966728 https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2179999763
    Which of the following components is NOT required for both Gibson assembly and forced cloning?
    a. Linearized plasmid
    b. Ligase
    c. Exonuclease
    d. Amplified insert

    Correct answer: c
    ]]>     2022-05-11 19:17:59 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2179999763     Change to multiple T/F https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2180002047 Answer true or false to this statement for each option below. This component is required for both Gibson Assembly and forced cloning.
    a. Linearized plasmid
    b. Ligase
    c. Exonuclease
    d. Amplified insert

    Correct answer: a-True, b-True, c- False, d- True
    ]]>     2022-05-11 19:19:56 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2180002047     https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2180004071 When designing a primer for Sanger sequencing, it is recommended that you allow a region of approximately 100 bp between the primer binding region and the target region for high quality sequencing data. Which of the following accurately describes the reasoning behind this consideration during primer design? 
    A. Chain-termination does not occur for approximately 100 bps, after which fluorescence signals are produced.
    B. DNA polymerase preferentially incorporates only ddNTPs for the first 100 bps.
    C. Signals from short products are difficult to detect resulting in poor quality of base-calling in the chromatogram.
    D. All of the above.

    Correct answer: C
    ]]>     2022-05-11 19:21:30 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2180004071     Remove "all of the above" as a response; adjust responses to similar length; remove negative phrasing; shorten stem https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2180005141 Which of the following describes the reasoning behind allowing a region of approximately (~) 100 bp between the primer binding region and the target region during primer design for Sanger sequencing?
    A. Chain termination occurs after ~100 bps have been incorporated.
    B. DNA polymerase preferentially incorporates ddNTPs for the first ~100 bps.
    C. Signals from products shorter than ~100 bps are difficult to detect.

    Correct answer: C
    ]]>     2022-05-11 19:22:28 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2180005141     Sophie Q2 Feedback https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181814520 These revision seem very helpful in considering the cognitive load required to process the question. 
    Great job!]]>     2022-05-12 19:12:19 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181814520     Christos Q1 Feedback https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181816706 Would you expect this to be a selected response question or essay?]]> 2022-05-12 19:13:59 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181816706 Alice Feedback https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181819373 Could you include a diagram that depicts the relationship of energy and nutrients for the possible answers. I initially think of differently shaded of arrows pointed in similar or opposite directions but it may not completely fit the concept. ]]> 2022-05-12 19:16:18 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181819373 Revised https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181819681 4. Which statement best describes the interaction of energy and nutrients within an ecosystem?
    a. Both energy and nutrients cycle through biotic, but not abiotic, components.
    b. Energy cycles through biotic components; nutrients cycle through abiotic components.
    c. Both energy and nutrients travel throughout ecosystems, but they follow separate, disconnected routes.
    d. Energy and nutrients enter biotic components through primary producers; energy flows one way and nutrients cycle.
    ]]>     2022-05-12 19:16:33 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181819681     https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181824153 2022-05-12 19:20:14 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181824153 Sophie Q1 Feedback smathews33 https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181828670 This is a good change to avoid the negative tense when asking a question. ]]> 2022-05-12 19:24:05 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181828670 Item Writing Checklist ccgoller https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181835278 2022-05-12 19:30:00 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181835278 https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181868924 You could try an ordering question (with careful phrasing)- put the three macromolecules in order of typical...]]> 2022-05-12 20:00:37 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181868924 https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181878460 Really good similar sentence structure between a & b and c & d, so you could do an either/or here instead of 4 choices so students don't get overwhelmed with reading so many responses. Good to test vocabulary at same time (if aligns with learning outcomes)]]> 2022-05-12 20:10:13 UTC https://padlet.com/askbio/dwg63hmwlnsctey7/wish/2181878460