Diferencias en la extraccion de ADN y ARN by MARCO MANUEL OCHOA MAGANA https://padlet.com/marcoochoa9503/demz1glpzwg6eakc MARCO MANUEL OCHOA MAGAÑA JULIETA OCHOA LIZARDI JONATHAN ULISES PATIÑO FARIAS AGROBIOTECNOLOGIA 5TO SEMESTRE en-us 2021-09-21 03:21:29 UTC 2024-10-12 07:27:00 UTC hello@padlet.com marcoochoa9503 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1755990795 2021-09-21 03:35:48 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1755990795 marcoochoa9503 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1755991419 2021-09-21 03:36:10 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1755991419 LISIS CELULAR: marcoochoa9503 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756006350 CTAB, SDS,Tris-HCI, EDTA, B- MERCAPTOETANOL

]]> 2021-09-21 03:44:46 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756006350 LISIS CELULAR: marcoochoa9503 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756007137 SARKOSYL, TRITON X, ISOCIANATO DE GUANDINA, FENOL]]> 2021-09-21 03:45:12 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756007137 PURIFICACION: marcoochoa9503 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756010253 CLOROFORMO]]> 2021-09-21 03:47:02 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756010253 PRECIPITACION: marcoochoa9503 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756010584 ALCOHOLES]]> 2021-09-21 03:47:14 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756010584 LAVADO: marcoochoa9503 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756010909 ETANOL 70%]]> 2021-09-21 03:47:22 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756010909 REHIDRATACION: marcoochoa9503 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756011240 H2O TRATADA CON DEPC]]> 2021-09-21 03:47:33 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756011240 ALMACENAMIENTO: marcoochoa9503 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756011688 -70°C]]> 2021-09-21 03:47:46 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756011688 PURIFICACION: marcoochoa9503 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756012616 PROTEINASAS
STES. ORGANICOS 
SALES]]> 2021-09-21 03:48:17 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756012616 PRECIPITACION: marcoochoa9503 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756012789 ALCOHOLES
ALCOHOLES + SAL]]> 2021-09-21 03:48:22 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756012789 LAVADO: marcoochoa9503 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756012957 ETANOL 70%]]> 2021-09-21 03:48:28 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756012957 REHIDRATACION: marcoochoa9503 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756013140 TE O H2O]]> 2021-09-21 03:48:34 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756013140 ALMACENAMIENTO: marcoochoa9503 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756013350 -20°C]]> 2021-09-21 03:48:41 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756013350 Extracción JulietaOchoa03 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756364041  Se usan solventes orgánicos como fenol y cloroformo.]]> 2021-09-21 07:28:03 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756364041 Extracción JulietaOchoa03 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756370252 Método del CTAB]]> 2021-09-21 07:31:16 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756370252 Antes de descongelarse JulietaOchoa03 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756377438 se adiciona la solución de lisis (2% de CTAB, 0.5% de PVP, 20mM EDTA pH 8.0, 100mM Tris base pH 8.0, 2.5M de NaCl).]]> 2021-09-21 07:34:49 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756377438 Antes de descongelarse JulietaOchoa03 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756381825 Adicionar sobre ella 10 volúmenes del buffer de lisis previamente precalentado. Homogenizar un par de veces antes de que descongele completamente.]]> 2021-09-21 07:36:57 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756381825 Transferir el sobrenadante JulietaOchoa03 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756387810 se agregan 0.5 volúmenes de fenol: cloroformo 5:1, 0.1 volúmenes de buffer de acetato de sodio 3M pH 5.2 y se homogeniza. Se centrifuga a 13,000 rpm por 10 minutos a 4oC.]]> 2021-09-21 07:39:50 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756387810 Transferir el sobrenadante JulietaOchoa03 https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756390355 Agregar 0.1 volúmenes de acetato de sodio 3M (pH 5.2), 0.5 volúmenes de fenol-cloroformo (5:1 v/v), homogenizar y centrifugar a 14,000rpm por 10 minutos a temperatura ambiente.]]> 2021-09-21 07:41:04 UTC https://padlet.com/marcoochoa9503/demz1glpzwg6eakc/wish/1756390355