https://padlet.com/NASerri/d2kkuk5iehx0xay6 Please answer all the question given in class here. en-us 2021-04-16 03:41:21 UTC 2021-12-17 09:55:50 UTC hello@padlet.com NASerri https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424124676 Watch this video and try to answer the questions given in the class session.
a. What type of immobilization used
b. Describe
c. What are the expected result (glucose & turbidity)
d. State pro and cons
e. Suggest method for glucose detection

]]> 2021-04-16 04:49:47 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424124676 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424550099 a. Entrapment
b. The yeast was entrapped in the matrices of calcium alginate  in the experiment. The matrices was produced by reacting a mixture of sodium alginate solution and enzyme with calcium chloride.
c.  The glucose level for immobilized enzymes is higher than the usual enzymes. The usual enzymes produced higher turbidity product.
d. Pros- ]]> 2021-04-16 08:26:06 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424550099 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424553343 a. Entrapment
b. Placement of an enzyme within the framework of a membrane or polymer matrix.
c. immobilized yeast will produce more glucose than free glucose
d. Pros: Easy to prepare and reusable.
Cons: yet can damaged the enzyme and reduce its activity.
e. Method suggestion:  DNS method

]]> 2021-04-16 08:27:27 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424553343 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424554666 - type of immobilization: Entrapment
- describe: Caging the enzyme by its covalent and enclosing the enzyme in membrane capsule. 
- expected result: more higher glucose will be produced by the immobilized yeast. the turbidity of immobilized yeast can be figured out too.
- pro: increase the enzymes stability
- cons: enzyme maybe damaged and the activity observed will reduced also
- method suggestion: DNS

]]> 2021-04-16 08:28:05 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424554666 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424554706 a. Entrapment
b. Yeast is being entrapped in the matrix which is alginate membrane
c. The expected result is the immobilized yeast will produce more glucose compare with the free yeast.
d. The pros of immobilized enzyme are improve the stability of enzyme and also can reduce the cost, whereas the cons of immobilized enzyme are lower enzyme activity compare to native enzyme.
e. Benedict's test. ]]> 2021-04-16 08:28:06 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424554706 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424555763 A - Entrapment
B - Enzyme (calcium chloride solution) attach to sodium alginate which is the inert.
C - glucose
D - Pro - Immobilized enzyme can be reuse
Cons - Lower enzyme activity
E - Benedict test

]]> 2021-04-16 08:28:35 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424555763 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424555791 a. Entrapment
b. The yeast produces an enzymes which is later entrapped into an membrane.
c. Presence of glucose by entrapment of cells in sodium alginate is higher.
d. Advantages are it produces high concentration and can be reused. Disadvantages are it can damage the structure of the enzyme.
e. DNS method.]]> 2021-04-16 08:28:36 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424555791 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424558830 A. Entrapment
B. Yeast mixed with sodium alginate. Then, the mixture is added dropwise in calcium chloride to form beads of calcium alginates.
C. Increase the production of glucose
D. pros: enzyme can be reuse.
cons: Damage the structure of enzyme
E. DNS method]]> 2021-04-16 08:29:56 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424558830 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424559998 a. entrapment method
b. the yeast was entrapped in sodium algitate.
c.the result the immobilized yeast will produce glucose more and and faster reaction compare to free yeast.
d. pros-   ]]> 2021-04-16 08:30:30 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424559998 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424560160 entrapment @ encapsulation

reaction : yeast produces an enzyme known as invertase to break down the sucrose into glucose and fructose

glucose from bead yeast is higher then free yeast

pro ; can be re use

con ; the enzyme may be damage due to collide of the enzyme itself

way to detect glucose : Dinitro Salicylic Acid (DNS) Method

]]> 2021-04-16 08:30:35 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424560160 syahirahrasol https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424560207 Type of immobilization 
- Entrapment
Reaction 
-Entrap the yeast in the membrane matrices
Expected result
-The production of glucose is increase and the turbidity also can be known.
Pros & cons
-Pros : can reduce the cost in the downstream reaction.
-Cons : low reaction rate
Suggestion method
-DNS method]]> 2021-04-16 08:30:36 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424560207 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424560989 a) Entrapment.
b) The yeast was trapped in the alginate gel. 
c) The immobilised yeast will produce high glucose. 
d) pros: enzyme can be recycled.
cons: It will reduce the enzyme activity.
e) dinitrosalicylic acid (DNS) method can detect the glucose.]]> 2021-04-16 08:30:59 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424560989 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424561697 a. Entrapment
b. It physically entrap the enzyme (in yeast) in the porous matrix (sodium alginate). The porous space allow the substrate pass through the enzyme hence breakdown reaction happen. 
c. Glucose concentration in the immobilised enzyme is greater than the free enzyme. Turbidity in immobilised enzyme is lower compared to free enzyme
d. Pros: easy handling 
Cons: reduce enzyme activity due to enzyme damaged
e. Benedict's test ]]> 2021-04-16 08:31:21 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424561697 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424562721 a. entrapment
b.polymer matrix entrap the yeast
c. high glucose yield
d.pro-enzyme more stable
con-less activity of enzyme

]]> 2021-04-16 08:31:51 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424562721 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424563170 1. encapsulation
2. yeast is mixed with sodium alginate solution  and reacted with calcium chloride performing capsule around the yeast suspension.  the capsule is reaction of alginate and calcium.
3. the beads will produce more glucose
4. pro, the enzyme bead can be reuse  
cons, enzyme will affected or damaged because of collided
5. DNS method, glucose strip ]]> 2021-04-16 08:32:03 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424563170 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424568646 a. entrapment
b. The yeast was entrapped in the matrices of calcium alginate.
c. The glucose level for immobilized enzyme is higher than the usual enzyme.
d. Pro: enzyme can be recycled.
Cons: The enzyme are exposed to damage
e. DNS method]]> 2021-04-16 08:34:31 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424568646 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424569324 a.Entrapment 
b. Yeast were entrapped in the membrane matrices 
c.The glucose that had been produced were increased and the turbidity can be figured out 
d.Pro-enzyme can be reused 
cons-can reduced enzyme activity and damaged the structure of enzyme 
e.DNS method]]> 2021-04-16 08:34:53 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424569324 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424569459 a) Entrapment 
b) yeast entrapped in matrix of sodium alginate
c) more glucose production
d) pros: enzyme can be reuse
cons: lower enzyme activity due to the damaged enzyme structure.
e) DNS method]]> 2021-04-16 08:34:57 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424569459 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424572665 a. Entrapment

b. The yeast were entrapped in the matrices.

c. The expected result is the production of the glucose will become increase.

d.  Pro: The enzyme can be reuse again
Cons: The structure of enzyme will be damage and its activity will also lower

e) DNS method]]> 2021-04-16 08:36:32 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424572665 dinahandsome14 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424582661 A. Immobilization used : entrapment
B. Between yeast drops that is mixed with sodium alginate solution and calcium chloride solution.
C. The presence of glucose in free yeast can not be identify meanwhile in immobilisation yeast can be seen by using the glucose strip test. As for the turbidity of each solutions, the immobilised yeast produces more glucose.
D. Pros: beads can be used and retrieved.
    Cons: The enzyme are exposed to damage
E. One way to detect glucose is by using DNS method]]> 2021-04-16 08:41:12 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424582661 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424583453 a. Entrapment
b. entrapped the yeast with sodium alignate
c. Can known the turbidity by production of glucose
d.Pros- entrapped inside a porous matrix. Bonds involved in stabilizing the enzyme to the matrix may non-covalent.
Cons- possibility of leakage of low molecular weight enzymes from the matrix.
e. hexokinase or DNS method

]]> 2021-04-16 08:41:34 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424583453 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424591457 a. Entrapment
b. It enclose the yeast into the matrices
c. Glucose production by the entrapment method is higher
d.pro : increase its resistance to various environmental changes. Ex: pH, temp, etc
- cons : the enzyme could been damage from collision
e. DNS method]]> 2021-04-16 08:45:04 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424591457 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424611249 a. Entrapment
b. The enzyme produced by the yeast was entrapped between the matrix's of sodium alginate and calcium chloride reactions
c. The glucose production will be higher than usual
d. pros- it can be reuse
    cons- can damage the enzyme structure.
e. DNS method]]> 2021-04-16 08:53:56 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1424611249 https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1425420958 a. entrapment
b, it will physically entraped in the yeast
c. more glucose will be produced
e. DNS method]]> 2021-04-16 13:49:56 UTC https://padlet.com/NASerri/d2kkuk5iehx0xay6/wish/1425420958