Analytical Procedures by Saambavi Ganesan

Limit of Quantitation (LOQ)

andyng183 — 2022-02-04 04:25:01 UTC

Linearity

cheryl16927 — 2022-02-04 04:25:05 UTC

ANALYTICAL METHOD VALIDATION

cheryl16927 — 2022-02-04 04:25:20 UTC

Precision

xholiinx — 2022-02-04 04:25:41 UTC

Limit of Detection (LOD)

andyng183 — 2022-02-04 04:26:07 UTC

xholiinx — 2022-02-04 04:28:04 UTC

Definition: it is the degree of scatter in measured values obtained from multiple sampling of the same sample

cheryl16927 — 2022-02-04 04:29:05 UTC

Definition: test results are directly proportional to the concentrate of analyte

Repeatability

xholiinx — 2022-02-04 04:29:42 UTC

precision under the same operating conditions over a short period of time

Intermediate Precision

xholiinx — 2022-02-04 04:30:23 UTC

evaluate the variance when the same sample is analyzed using two or more different instruments, or by//between lab technicians, or between different days --> Precision expresses within-laboratories variations: different days, different analysts, different equipment, etc.

Reproducibility

xholiinx — 2022-02-04 04:31:20 UTC

the same sample getting tested by two or more laboratories and the results will be evaluated (similar to intermediate precision)

andyng183 — 2022-02-04 04:32:21 UTC

Definition: The smallest amount or the lowest concentration of a substance that is possible to be determined by means of a given analytical procedure with the established accuracy, precision and uncertainty.

gsaambavi — 2022-02-04 04:32:39 UTC

Definition: It is the smallest amount or concentration of analyte in the test sample that can be reliability distinguished from ZERO

--> Yes or No

Robustness

andyng183 — 2022-02-04 04:34:42 UTC

cheryl16927 — 2022-02-04 04:35:27 UTC

reflects a range to compare different results

andyng183 — 2022-02-04 04:37:00 UTC

Definition: The ability of a specific substance to withstand or overcome adverse conditions or rigorous testing / ability to resist change

Specificity

andyng183 — 2022-02-04 04:38:28 UTC

xholiinx — 2022-02-04 04:38:40 UTC

andyng183 — 2022-02-04 04:40:53 UTC

Definition: Ability to access unequivocally the analyte in the presence of impurities

gsaambavi — 2022-02-04 04:48:48 UTC

If the analysis result is,
1) > LOQ --> Able to state the quantitative result and uncertainty

2) Between LOD and LOQ --> Analyte is present in the sample

3) Analyte is not detected. Cannot be written that the analyte is not present in the sample as the concentration of the analyte can be so low that the analysis method cannot distinguish it from the noise

xholiinx — 2022-02-04 04:59:23 UTC

xholiinx — 2022-02-04 05:09:05 UTC

xholiinx — 2022-02-04 05:11:58 UTC

xholiinx — 2022-02-04 05:12:37 UTC