Module 2 by Laura Bacete https://padlet.com/laurabacete/BI2015_G1_M2 What should be in the report? en-us 2021-10-01 11:53:55 UTC 2021-10-21 08:51:18 UTC hello@padlet.com https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799005712 Explain techniques: CRISPR-Cas9, blue white screening, plasmid isolation.
Theory about: lacZ gene, B-gal]]> 2021-10-07 07:33:26 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799005712 https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799252449 References! 

]]> 2021-10-07 09:37:51 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799252449 Gene editing https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799254759 2021-10-07 09:39:04 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799254759 Result of blue/white screening (colour of colonies on the plates). https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799257010 2021-10-07 09:40:19 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799257010 Reference to lab booklet. https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799258506 2021-10-07 09:41:11 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799258506 DNA concentrations measured on nanodrop. https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799259183 2021-10-07 09:41:30 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799259183 https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799259934 Did we get the results that were expected- why not?

]]> 2021-10-07 09:41:53 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799259934 How was the function of lacZ tested using CRISPR. https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799260978 2021-10-07 09:42:26 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799260978 Plasmid concentration https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799261818 2021-10-07 09:42:55 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799261818 Hypothesis https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799263955 2021-10-07 09:44:05 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799263955 Plasmid concentration data https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799266128 Nanodroplet A260/A280 ratio]]> 2021-10-07 09:45:18 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799266128 sgRNA https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799266786 2021-10-07 09:45:39 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799266786 Bacterial transformation https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799267943 2021-10-07 09:46:18 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799267943 Explain the blue color https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799268122 2021-10-07 09:46:23 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799268122 Lac operon - function https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799268374 2021-10-07 09:46:31 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799268374 Plasmids https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799270196 2021-10-07 09:47:33 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799270196 How/why test for function of a gene https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799273015 2021-10-07 09:49:03 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799273015 The aim of the experiment https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799273703 2021-10-07 09:49:22 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799273703 Homology directed repair https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799275241 2021-10-07 09:50:07 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799275241 What can be improved? https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799340982 Future steps]]> 2021-10-07 10:28:14 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1799340982 https://padlet.com/laurabacete/BI2015_G1_M2/wish/1823691867 Crispr-cas: background, discovered as a bacterial adaptive immune system. ]]> 2021-10-18 09:54:41 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1823691867 https://padlet.com/laurabacete/BI2015_G1_M2/wish/1823696323 Explanation of different phenotypes observed in dishes A-D]]> 2021-10-18 09:57:46 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1823696323 Plasmids https://padlet.com/laurabacete/BI2015_G1_M2/wish/1832895623 pLZDonor (control)
pLZDonorGuide]]> 2021-10-21 08:37:19 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1832895623 Colour of colonies the first and second week https://padlet.com/laurabacete/BI2015_G1_M2/wish/1832897559 2021-10-21 08:38:23 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1832897559 Nanodrop: Nevne konsentrasjon og tetthet, dette er ikke hovedfokuset i modulen. https://padlet.com/laurabacete/BI2015_G1_M2/wish/1832919982 2021-10-21 08:51:18 UTC https://padlet.com/laurabacete/BI2015_G1_M2/wish/1832919982