https://padlet.com/st12012881/bwcu02nx4vh6o356 By: Dara and Sol en-us 2022-05-11 16:59:06 UTC 2025-11-01 02:56:25 UTC hello@padlet.com st12012881 https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179792218 2022-05-11 16:59:38 UTC https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179792218 st1205498 https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179797520 the process of PCR amplifies DNA
1. sample is first heated so the DNA denatures or separates into two pieces of single-stranded DNA
2. an enzyme called "Taq polymerase" synthesizes - builds - two new strands of DNA ( using the original strands as templates)
3. process results in the duplication of the original DNA, with each of the new molecules containing one old and one new strand of DNA. ]]> 2022-05-11 17:03:09 UTC https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179797520 st1205498 https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179800789 this process occurs 30- 40 times leading to more than 1 BILLION DNA duplicates!]]> 2022-05-11 17:05:18 UTC https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179800789 st1205498 https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179809891 PCR occurs in laboratories where it is automated by a machine called thermocycler and occurs in a couple of hours. Most mapping techniques in the Human Genome Project (HGP) relied on PCR.]]> 2022-05-11 17:11:14 UTC https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179809891 st1205498 https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179810886 2022-05-11 17:11:53 UTC https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179810886 st12012881 https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179811647 Researchers create copies of a particular gene for further application:
     - sequencing
     - mutagenesis
     - genotyping]]> 2022-05-11 17:12:22 UTC https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179811647 st1205498 https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179818974 2022-05-11 17:17:17 UTC https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179818974 st12012881 https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179820795 Gene cloning also allows for the creation of biopharmaceuticals, for instance, insulin or HGH -> synthetic creation. 

Also, it is used in gene therapy, when patients aren't able to functionally form a specific gene.]]> 2022-05-11 17:18:30 UTC https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2179820795 st12012881 https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2181496600 2022-05-12 15:44:15 UTC https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2181496600 st12012881 https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2181596232 "Gene cloning is a common practice in molecular biology labs that is used by researchers to create copies of a particular gene for downstream applications... The traditional technique for gene cloning involves the transfer of a DNA fragment of interest from one organism to a self-replicating genetic element, such as a bacterial plasmid."

https://www.bio-rad.com/es-pa/applications-technologies/introduction-gene-cloning-analysis?ID=LUSNKO4EH ]]> 2022-05-12 16:45:39 UTC https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2181596232 st1205498 https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2181616942  PCR is valuable in a number of laboratory and clinical techniques, including DNA fingerprinting, detection of bacteria or viruses (particularly AIDS), and diagnosis of genetic disorders]]> 2022-05-12 16:58:49 UTC https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2181616942 st1205498 https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2181654749 https://quizizz.com/join/quiz/627cf9ad3a89a8001d9ae68e/start?studentShare=true

]]> 2022-05-12 17:22:46 UTC https://padlet.com/st12012881/bwcu02nx4vh6o356/wish/2181654749