Immunology by https://padlet.com/msegovia4/b9hvp5yy4kkmqf51 Why Immunology is important for you? en-us 2021-07-01 20:52:36 UTC 2023-05-24 22:24:28 UTC hello@padlet.com https://padlet.net/icons/png/1f489.png Did you know that some determinations that are usually made in clinical laboratory (and even at home!) involve immunological techniques? Have you ever used a quick test for something? msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1633903940 2021-07-01 21:04:18 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1633903940 Search commercial websites for 3 tests you can buy to diagnose yourself a medical condition (not just diseases) at home. Discuss with your classmates which of them are immunology-based. msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1633917677 2021-07-01 21:24:48 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1633917677 BLOOM’S TAXONOMY msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1633973565 2021-07-01 22:50:54 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1633973565 Match the relationships between items in both columns. On the left, laboratory tests. On the right, immunological or non-immunological tests. msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635233997 2021-07-02 19:13:16 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635233997 Work in groups of 4 people. Build a vocabulary of 25 terms, arranged alphabetically, on immunological techniques, in English. msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635243616 2021-07-02 19:28:06 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635243616 Watch the following video and discuss with your classmates whether or not an immune test is displayed. Write your final conclusions. msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635249990 2021-07-02 19:37:19 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635249990 Read the following text and explain what technique is it talking about. msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635258155 Extracellular vesicles (EVs) provide an invaluable tool to analyse physiological processes because they transport, in biological fluids, biomolecules secreted from diverse tissues of an individual. EV biomarker detection requires highly sensitive techniques able to identify individual molecules. However, the lack of widespread, affordable methodologies for high-throughput EV analyses means that studies on biomarkers have not been done in large patient cohorts. To develop tools for EV analysis in biological samples, we evaluated here the critical parameters to optimise an assay based on immunocapture of EVs followed by flow cytometry. We describe a straightforward method for EV detection using general EV markers like the tetraspanins CD9, CD63 and CD81, that allowed highly sensitive detection of urinary EVs without prior enrichment. In proof-of-concept experiments, an epithelial marker enriched in carcinoma cells, EpCAM, was identified in EVs from cell lines and directly in urine samples. However, whereas EVs isolated from 5–10 ml of urine were required for western blot detection of EpCAM, only 500 μl of urine were sufficient to visualise EpCAM expression by flow cytometry. This method has the potential to allow any laboratory with access to conventional flow cytometry to identify surface markers on EVs, even non-abundant proteins, using minimally processed biological samples.]]> 2021-07-02 19:49:44 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635258155 How do you think it could be named in English an immunological test based in the use of fluorescence to identify the immunocomplex? msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635265857 2021-07-02 20:01:43 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635265857 Analyze the following terms on laboratory techniques and indicate which of them do you think are or are not immunologically based tests msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635269102 2021-07-02 20:07:07 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635269102 Fill the gaps with the correct name of the immunological test msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635271903 2021-07-02 20:11:51 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635271903 msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635297246 2021-07-02 20:54:57 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1635297246 Check your knowledge msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1636364779 Allergies]]> 2021-07-04 20:21:00 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1636364779 Primary or secondary techniques? msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1636394615 I would suggest to discuss about the convenience of using primary (more sensitive) or secondary techniques (less sensitive) to determine the main analytes tha allow to diagnos Type I Hypersensitivities in clinical laboratories.
It would be done by using Answergarden: https://answergarden.chFeedback tool
(I can not be able to develop because webpage of answergarden  don't work at this moment).
However, I would send to students several questions to center a debate framework:
1- What are the most important analytes to identify Type I HS?
2- Rank immunological techniques based on their sensitivity
3- What are the usual concentrations in serum of the above mentioned main analytes?
 ]]> 2021-07-04 21:49:56 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1636394615 Read the abstracts provided and extract the recurrent terms to talk about Type I Hipersensitivity (HS). msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1636398258 2021-07-04 22:00:56 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1636398258 Case reports msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1636419547 2021-07-04 23:06:55 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1636419547 Read the text provided about Type I Hypersensitivities and write an abstract containing all important information in less than 250 words. msegovia4 https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1636421586 2021-07-04 23:11:25 UTC https://padlet.com/msegovia4/b9hvp5yy4kkmqf51/wish/1636421586