https://padlet.com/amayes81/b4ha8grwg2pqp1x en-us 2023-03-07 00:16:08 UTC 2023-03-07 00:51:26 UTC hello@padlet.com amayes81 https://padlet.com/amayes81/b4ha8grwg2pqp1x/wish/2505969873 Desired genes for plants are inserted into the Ti plasmid, the most common genes are herbicide and insecticide. ]]> 2023-03-07 00:25:52 UTC https://padlet.com/amayes81/b4ha8grwg2pqp1x/wish/2505969873 amayes81 https://padlet.com/amayes81/b4ha8grwg2pqp1x/wish/2505975664 The Ti plasmid is inserted into the Agrobacterium through methods like heat shock, sonification, or electroportation.

*heat shock method is best to use because it decreases the chances of creating a crown gall]]> 2023-03-07 00:30:50 UTC https://padlet.com/amayes81/b4ha8grwg2pqp1x/wish/2505975664 amayes81 https://padlet.com/amayes81/b4ha8grwg2pqp1x/wish/2505993619 The leaf of the plant is wounded so agrobacterium can attach to the plant cell wall and can transfer the TDNA of the plasmid that contains your desired genes. ]]> 2023-03-07 00:45:11 UTC https://padlet.com/amayes81/b4ha8grwg2pqp1x/wish/2505993619 amayes81 https://padlet.com/amayes81/b4ha8grwg2pqp1x/wish/2505996717 This step is not required, but the leaf fragment can continue to thrive on agar plates to create a callus. This callus that contains the desired genes canbe used to create plants that grow up with the gene.]]> 2023-03-07 00:47:50 UTC https://padlet.com/amayes81/b4ha8grwg2pqp1x/wish/2505996717 amayes81 https://padlet.com/amayes81/b4ha8grwg2pqp1x/wish/2506000872 This method is also known as the agrobacter mediated gene transfer. Agrobacter is a bacteria found in soil that enters wounds of plants and can transfer DNA. ]]> 2023-03-07 00:51:15 UTC https://padlet.com/amayes81/b4ha8grwg2pqp1x/wish/2506000872