eDNA by Susan https://padlet.com/uraskys/environmentaldna An introduction to how environmental DNA is used to collect scientific data. en-us 2017-02-19 17:45:10 UTC 2017-02-26 19:17:56 UTC hello@padlet.com https://padlet-assets.s3.amazonaws.com/icons/Clouds.png What is eDNA? uraskys https://padlet.com/uraskys/environmentaldna/wish/154816333 DNA released from an organism into the environment (hair, saliva, mucus, skin, etc.)

]]> 2017-02-19 19:09:33 UTC https://padlet.com/uraskys/environmentaldna/wish/154816333 Hello... uraskys https://padlet.com/uraskys/environmentaldna/wish/154816800 eDNA can be used to track secretive, rare, and small species ]]> 2017-02-19 19:16:58 UTC https://padlet.com/uraskys/environmentaldna/wish/154816800 Blocking Intruders uraskys https://padlet.com/uraskys/environmentaldna/wish/156201746 eDNA samples can be used to determine the spread of invasive species and provide scientists with an idea of potential containment areas. (Byelmans, et al., 2016). Samples are taken from increasingly distant areas from the main population of invasives to determine the affected area.]]> 2017-02-25 18:19:22 UTC https://padlet.com/uraskys/environmentaldna/wish/156201746 How do scientists use eDNA? uraskys https://padlet.com/uraskys/environmentaldna/wish/156202273 This SoundCloud recording gives a good overview of how scientists in Oregon are using eDNA. Potentially, scientists can identify specific species and the abundance of those species from a small set of samples. ]]> 2017-02-25 18:29:37 UTC https://padlet.com/uraskys/environmentaldna/wish/156202273 Ghosting uraskys https://padlet.com/uraskys/environmentaldna/wish/156202930 Lack of DNA markers for certain species in a sample does not mean that species is not present in the environment. It simply is not present in that specific sample.]]> 2017-02-25 18:42:59 UTC https://padlet.com/uraskys/environmentaldna/wish/156202930 How does it all come together? uraskys https://padlet.com/uraskys/environmentaldna/wish/156203712 Listen to Kellie...she knows what's up.]]> 2017-02-25 19:01:49 UTC https://padlet.com/uraskys/environmentaldna/wish/156203712 Oh, there you are!! uraskys https://padlet.com/uraskys/environmentaldna/wish/156204339 Scientists are using eDNA to help track populations of endangered species, like the Yangtze River dolphin (Ma et al., 2016).]]> 2017-02-25 19:15:08 UTC https://padlet.com/uraskys/environmentaldna/wish/156204339 Long term survey says... uraskys https://padlet.com/uraskys/environmentaldna/wish/156204542 eDNA is helping scientists study the biodiversity of aquatic environments over time (Bista et al., 2017). By sampling the same areas multiple times over the course of a year or longer, scientists can monitor trends in biodiversity.]]> 2017-02-25 19:19:41 UTC https://padlet.com/uraskys/environmentaldna/wish/156204542 eDNA is extracted by: uraskys https://padlet.com/uraskys/environmentaldna/wish/156204873 collecting samples (most commonly from aquatic environments) and analyzed for specific DNA markers which indicate the presence of certain species. Matt will give you an in-depth look at the extraction process]]> 2017-02-25 19:28:24 UTC https://padlet.com/uraskys/environmentaldna/wish/156204873 Water, water everywhere uraskys https://padlet.com/uraskys/environmentaldna/wish/156205922 A recent study compared the efficacy of eDNA in comparison with other sampling forms. It was discovered the DNA markers in water had a much longer persistence than eDNA in sediment (Baldigo et al., 2016).]]> 2017-02-25 19:56:03 UTC https://padlet.com/uraskys/environmentaldna/wish/156205922 Magical Mystical Moving DNA uraskys https://padlet.com/uraskys/environmentaldna/wish/156206325 As many of eDNA samples are taken in moving water, it is still unknown how far traceable DNA has traveled in the water (Roussel et al., 2015).]]> 2017-02-25 20:06:14 UTC https://padlet.com/uraskys/environmentaldna/wish/156206325 Why use eDNA? uraskys https://padlet.com/uraskys/environmentaldna/wish/156206705 Sampling in aquatic environments allows for scientists to search for specific DNA markers, identifying certain species. It can also provide an overview of biodiversity in a body of water. Additionally, it does not in any way disturb the environment, so it can be a good option for fragile and protected spaces.]]> 2017-02-25 20:16:59 UTC https://padlet.com/uraskys/environmentaldna/wish/156206705 Citations uraskys https://padlet.com/uraskys/environmentaldna/wish/156264477 "Filtering water samples for eDNA analysis" by ChicagoUSACE is licensed under CC BY 2.0
"Ghost" by Jordi Carrasco is licensed under CC BY 2.0
"Little boy responds to Adele's Hello" by VineMonster is licensed under Standard YouTube License
"Scientists move to make eDNA the next big thing for Oregon stream" by EarthFix is licensed under CC BY 2.0
"Working with environmental DNA (eDNA)" by USDA Forest Service is licensed under Standard YouTube License


Baldigo, B., Sporn, L.A., George, S., & Ball, J. (2017). Efficacy of environmental DNA to detect and quantify brook trout populations in headwater streams of the Adirondack Mountains, New York. Transactions of the American Fisheries Society, 146(1), 99-111.

Bista, I., Carvalho, G. R., Walsh, K., Seymour, M., Hajibabaei, M., Lallias, D., &Creer, S. (2017). Annual time-series analysis of aqueous eDNA reveals ecologically relevant dynamics of lake ecosystem biodiversity. Nature Communications, 8, 14087. http://doi.org.proxy.lib.miamioh.edu/10.1038/ncomms14087

Bylemans, J., Furlan, E., Gleeson, D., Pearce, L., & Daly, T. (2016). Improving the containment of a freshwater invader using environmental DNA (eDNA) based monitoring. Biological Invasions, 18(10), 3081-3089. doi:10.1007/s10530-016-1203-5

Ma, H., Stewart, K., Zhao, J., Lougheed, S., Wang, Y., & Zheng, J. (2016). Characterization, optimization, and validation of environmental DNA (eDNA) markers to detect an endangered aquatic mammal. Conservation Genetics Resources, 8(4), 561-568. doi:10.1007/s12686-016-0597-9 

Roussel, J.-M., Paillisson, J.-M., Tréguier, A. and Petit, E. (2015). The downside of eDNA as a survey tool in water bodies. Journal of Applied Ecology, 52, 823–826. doi:10.1111/1365-2664.12428

U.S. Geological Survey. (2013). Application of environmental DNA for inventory and monitoring of aquatic species. Retrieved from https://pubs.usgs.gov/fs/2012/3146/pdf/fs2012-3146.pdf
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