https://padlet.com/spatri11/axk33xz8ptsxwta0 en-us 2022-01-27 17:22:44 UTC 2022-01-27 22:40:08 UTC hello@padlet.com spatri11 https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016390880 3. DNA is removed from cellular debris and purified
    a) spun in centrifuge to collect debris at bottom of tube
    b) supernatant is transferred to new tube and mixed with silica
    c) incubate to allow DNA to bind to silica 
    d) spun in centrifuge to collect DNA/silica pellet at bottom of tube
     e) remove supernatant
     f) washed and spun twice to remove impurities
     g) remove supernatant
     h) add water and incubate to release DNA from silica  pellet
      i) spin and collect silica resin at bottom of tube
      j) remove supernatant (DNA) and store on ice at -20ºC


]]> 2022-01-27 17:24:40 UTC https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016390880 spatri11 https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016421018 2022-01-27 17:38:52 UTC https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016421018 spatri11 https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016422110 1. Obtain a small sample ]]> 2022-01-27 17:39:22 UTC https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016422110 spatri11 https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016422687 2. Cell walls and membranes are broken down through manual crushing and heat]]> 2022-01-27 17:39:34 UTC https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016422687 spatri11 https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016433434 4. Multiple copies of target gene (COI or rbcL) are made using polymerase chain reaction (PCR). 
   a) sample of extracted DNA is transferred to a tube with PCR reagents, mixed, and placed in a thermocycler. 
b) PCR cycle is repeated at least 15 times]]> 2022-01-27 17:44:35 UTC https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016433434 spatri11 https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016442247 5. Gel Electrophoresis 
    a) pipette is used to load DNA sample mixed with loading dye and florescent stain into wells of agarose gel
    b) electrical charge caused DNA fragments to move through the gel, and they separate by size
c) gel is illuminated to see where DNA fragments are located]]> 2022-01-27 17:48:37 UTC https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016442247 spatri11 https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016447510 6. DNA sequenced once PCR band presence is confirmed; done by commercial sequencing company]]> 2022-01-27 17:51:05 UTC https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016447510 spatri11 https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016450635 7. DNA analyzed using DNA Subway; can be used to identify closely related sequences]]> 2022-01-27 17:52:31 UTC https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016450635 spatri11 https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016456375 2022-01-27 17:55:09 UTC https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016456375 spatri11 https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016460712 DNA barcoding is a simple, accessible scientific method for reliable identification of species]]> 2022-01-27 17:57:06 UTC https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016460712 spatri11 https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016898286 Knowing an organism's DNA sequence can be helpful in determining their relatedness to your subject of interest, therefore allowing you to pick a model organism in the lab]]> 2022-01-27 22:40:04 UTC https://padlet.com/spatri11/axk33xz8ptsxwta0/wish/2016898286