My radiant padlet by https://padlet.com/lillipavicic/avsvsct3t1vxkwp3 en-us 2022-12-04 15:31:12 UTC 2026-02-03 02:56:10 UTC hello@padlet.com lillipavicic https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408101907 2022-12-04 16:08:27 UTC https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408101907 Experimental Flow qintianwang https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408114905 Grouping:

  • Experimental Autoimmune Encephalomyelitis (EAE) induction in 0 M
    • Two control groups: age-matched controls (AMC) either received subdermal inoculation with complete Freund's adjuvant (CFA, n = 10) or did not receive any injections (n = 10) during the experiment.
  • Mice that had been exposed to EAE were split into two groups and performed intraocular injections in the 10 M. 
    • In one group, 1 μl of scAAV-NDI1 triple capsid mutant was intravitreally injected
    • The other group received 1 μl of scAAVmCherry 
    • As extra injection controls, 1 μl of scAAV-mCherry was injected into CFA mice.

Timeline:

    • First treatment of control groups took place in 0 M to see if other factors may have potentially had an effect on the decrease in PERG and confirm the effect of the treatment in the experimental group.

    • PERG/SD-OCT was performed between 1 M and 9 M to measure if there were changes in normal visual function

    • Second treatment of control groups took place in 10 M to see if the injection of NDI1 can improve visual function in EAE mice and the PERG and SD-OCT were used to test the effect in 12 M

    • Mice were euthanized 2 months post-AAV injections to perform other relevant tests and analysis.
     
]]> 2022-12-04 16:31:42 UTC https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408114905 What is Multiple Sclerosis (MS)? lillipavicic https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408115910 https://www.news-medical.net/health/Types-of-Multiple-Sclerosis-%28MS%29.aspx]]> 2022-12-04 16:33:22 UTC https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408115910 Methods eesharehman https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408116937 2022-12-04 16:35:00 UTC https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408116937 Results eesharehman https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408117004 2022-12-04 16:35:06 UTC https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408117004 Discussion eesharehman https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408117059 2022-12-04 16:35:11 UTC https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408117059 INTRODUCTION lillipavicic https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408118965 2022-12-04 16:38:32 UTC https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408118965 Pattern Electroretinography (PERG) qintianwang https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408151777 2022-12-04 17:34:57 UTC https://padlet.com/lillipavicic/avsvsct3t1vxkwp3/wish/2408151777