Liver Enzyme Lab by Sanskriti Kulshreshtha https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j Honors Biology en-us 2017-06-28 20:55:55 UTC 2025-10-04 12:31:33 UTC hello@padlet.com https://padlet-assets.s3.amazonaws.com/icons/Growing.png Purpose/Problem sanskriti_kulshreshtha https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j/wish/177684998 To systematically prove that the liver can break down peroxide into oxygen and water using enzymes.

]]> 2017-06-28 20:57:38 UTC https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j/wish/177684998 Introduction sanskriti_kulshreshtha https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j/wish/177685033 Each cell in our body produces peroxide which is poisonous for us. To deal with this peroxide each cell also produces catalase an enzyme that neutralizes peroxide into oxygen and water. The kidney produces the enzymes.

]]> 2017-06-28 20:58:27 UTC https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j/wish/177685033 Materials sanskriti_kulshreshtha https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j/wish/177685054 5 Test Tubes

Water

Piece of Liver

Peroxide 

 Lighter

2 Wooden Splints

]]> 2017-06-28 20:58:59 UTC https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j/wish/177685054 Procedure sanskriti_kulshreshtha https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j/wish/177685075 1.      Add water into test tube number one up to the 2cm. mark to act as a control.
2.      Add a piece of liver to test tube 1, pushing it down till it hits the water. Record the reaction, if any.
3.      Fill test tube number 2 with peroxide to 2cm mark and add liver, push liver down till it reaches peroxide.
4.      Record the reaction, if any.
5.      Now we need to prove that the reaction that occurred is water and oxygen. To do this put peroxide in 3rd tube up to 2cm mark.
6.      Light wooden splint on fire and let it burn until it glows red and place it into peroxide.
7.      Record reaction, if any. 
8.      There was no reaction, this proves that peroxide isn't flammable. Now put 2cm of peroxide into 4th test tube, add the liver, remember to push it down. 
9.      To prove the reaction is oxygen take the wooden splint and light it till it lights red and ten place in peroxide reaction.
10.   The fire proves oxygen and water is created because pure oxygen when lit causes fire. Next boil a piece of liver (when too much heat is applied then the proteins denature)
11.   Put water and boiled liver in last test tube then put peroxide. Record reaction, if any.
 

 

]]> 2017-06-28 20:59:31 UTC https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j/wish/177685075 Results sanskriti_kulshreshtha https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j/wish/177685442  Test Tube 1 (water and liver)- No reaction 
Test Tube 2 (liver and peroxide)-Bubbly reaction, perhaps with oxygen and water 
Test Tube 3 (peroxide and wooden stick on fire) -No reaction 
Test Tube 4 (liver, peroxide, wooden stick on fire) -Bubbly reaction, lights flame 
 Test Tube 5 (boiled liver, water, peroxide)-No reaction 
 ]]> 2017-06-28 21:05:13 UTC https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j/wish/177685442 Discussion sanskriti_kulshreshtha https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j/wish/177685675
1) What are enzymes?  What are their roles in chemical reactions?


Enzymes are macromolecular biological catalysts. They excite and speed up chemical reactions.


 


 2) Describe what happened to test tube 2 when hydrogen peroxide was added to the liver.  What gas was produced?  What liquid was left behind?


When hydrogen peroxide was added to the liver a enzyme-substrate reaction took place. Oxygen was created and water was left behind.


3) What effect does the red hot wood have on test tube 4? WHY is the flame being produced? 


In test tube 4 the red hot wood caused a flame to be produced, the flame is being produced because pure oxygen when lit causes fire.


 


4) What effect does temperature have on the enzymes (Test tube 5)?


The temperature caused the proteins in the enzyme to denature and not fit with the substrate.

]]> 2017-06-28 21:09:55 UTC https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j/wish/177685675 Conclusion sanskriti_kulshreshtha https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j/wish/177685685
Enzymes are macromolecular biological catalysts. They excite and speed up chemical reactions. The enzyme catalase found in the liver help detoxify our bodies of peroxide. In our experiment when hydrogen peroxide was added to the liver a enzyme-substrate reaction took place. Oxygen was created and water was left behind. This proves that the reaction in the liver neutralizes peroxide. 

]]> 2017-06-28 21:10:09 UTC https://padlet.com/sanskriti_kulshreshtha/amvjupgbf71j/wish/177685685