DNA extraction by

DNA extraction by https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp en-us 2021-05-18 13:10:23 UTC 2026-02-25 23:11:25 UTC hello@padlet.com Group A) Which of the following statements about base pairing in DNA is incorrect? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610076011 a. Purines always base pairs with pyrimidines X
b. Adenine binds to guanines
c. Base pairs are stabilized by hydrogen bonds.
d. Base pairing occurs at the interior of the double helix.

]]> 2021-06-16 14:15:48 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610076011 Group A) During denaturation of DNA, which of the following happens? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610078069 a. Hydrogen bonds between complementary bases break X
b. Phosphodiester bonds break within the sugar-phosphate backbone.
c. Hydrogen bonds within the sugar-phosphate backbone break.
d. Phosphodiester bonds between complementary bases break.

]]> 2021-06-16 14:16:37 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610078069 Group B) During denaturation of DNA, which of the following happens? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610309563 a. Hydrogen bonds between complementary bases break X
b. Phosphodiester bonds break within the sugar-phosphate backbone.
c. Hydrogen bonds within the sugar-phosphate backbone break.
d. Phosphodiester bonds between complementary bases break.

]]> 2021-06-16 15:57:04 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610309563 Group C) DNA strands run _____ in relation to each other. marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610312930 a. antiparallel X
b. parallel
c. perpendicular
d. both a and b ]]> 2021-06-16 15:58:47 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610312930 Group C) A nucleotide in DNA is composed of _____. marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610315262 a. only a deoxyribose sugar and a phosphate
b. only a deoxyribose sugar and a nitrogen base
c. a deoxyribose sugar, a phosphate, and a nitrogen base X
d. none of the above]]> 2021-06-16 15:59:47 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610315262 Group D) Between the two strands of a DNA segment the nitrogen bases are held together by_____. marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610316588 a. covalent bonds
b. hydrogen bonds X
c. ionic bonds
d. metallic bonds]]> 2021-06-16 16:00:29 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610316588 Group D) Nitrogen bases pair with bases that are _____. marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610317891 a. available
b. complimentary x
c. identical
d. both b and c ]]> 2021-06-16 16:01:11 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610317891 Group E) A piece of double stranded DNA has 30% A, what will be the % of G? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610320155 a. 30%
b. 40%
c. 20% X
d. 70%
e. not enough information to answer ]]> 2021-06-16 16:02:18 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610320155 Group E) Genomic DNA is transcribed resulting in the production of mRNA. marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610322342 a. Transcribed, mRNA
b. Translated, tRNA
c. Transcribed, DNA
d. Translated, protein
e. Replicated, mRNA]]> 2021-06-16 16:03:25 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610322342 Group F) Which of the following statements about double-stranded DNA is false? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610324761 a. The backbone contains alternating sugar and phosphate groups.
b. The two strands have an antiparallel orientation.
c. The helix has two grooves called the major groove and the minor groove.
d. The bases are on the outside of the helix. XX
e. The strands are formed by nucleotides linked together via phosphodiester bonds]]> 2021-06-16 16:04:27 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610324761 Group F) DNA exists in a double-stranded form whereas RNA is mainly a single stranded molecule. What is the likely reason for DNA being double stranded? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610330480 a) RNA strands cannot form base pairs
b) Double stranded DNA is a more stable structure X
c) DNA cannot exist in the single stranded form
d) It is easier to replicate double stranded DNA than single stranded RNA]]> 2021-06-16 16:07:24 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610330480 Group F) Analysis of a sample of DNA found that 20% of the bases were adenine. What percentage of the bases would be pyrimidines? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610333716 a. 20%
b. 30%
c. 60%
d. 50% XX]]> 2021-06-16 16:09:02 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610333716 Group B) Thymine comprised 36% of the nitrogen bases present in a sample of double stranded DNA. What was the percentage of guanine in the sample? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610338905 a. 14% X
b. 28%
c. 36%
d. 64% ]]> 2021-06-16 16:11:36 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610338905 Group A) One similarity between DNA and messenger RNA molecules is that they both contain marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610356890 a. the same sugar
b. genetic codes based on sequences of bases X
c. a nitrogenous base known as uracil
d. double-stranded polymers ]]> 2021-06-16 16:20:11 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610356890 Group B) Name the RNA molecules which is used to carry genetic information copied from DNA? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610361259 a. tRNA
b. mRNA X
c. rRNA
d. snRNA]]> 2021-06-16 16:22:26 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610361259 Group C) Which of the following RNA molecule convert information stored in the nucleic acid to protein? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610363971 a. mRNA X
b. snRNA
c. rRNA
d. tRNA]]> 2021-06-16 16:23:50 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1610363971 Group D) How many nitrogenous bases are there in DNA? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1612507138 a. 1
b. 2
c. 3
d. 4 x]]> 2021-06-17 15:28:16 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1612507138 Group E) DNA looks like a “twisted ladder”. If the “rungs” of the ladder are made up of nitrogenous bases, what are the hand rails or backbone of the ladder made up of? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1612510878 a. Sugars and Salts
b. Phosphates
c. Sugars
d. Sugars and Phosphates X]]> 2021-06-17 15:30:03 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1612510878 Group A) RNA molecules marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1617326082 a. Contain ribose instead of deoxyribose X
b. Are less abundant than DNA in cells
c. Can be processed in different ways
d. Are usually more than 3000 nucleotides long
e. Are more stable than DNA molecules]]> 2021-06-21 08:51:40 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1617326082 Group B) Ribosomal RNAs marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1617328272 a. Make up ≈ 20% of all RNA in a cell
b. Are involved in synthesis of proteins X
c. Are molecules that normally are not processed X
d. Bind ribosomal proteins X ]]> 2021-06-21 08:53:32 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1617328272 Group C) The major form of DNA in cells is: marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1617526334 1. A-DNA
b. B-DNA X
c. C-DNA
d. Z-DNA ]]> 2021-06-21 12:14:39 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1617526334 Group E) After Phenol-chloroform-extraction and centrifugation (pH 4.2), your tube looks like this. Which fraction should you collect if you want the RNA marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1617530612 a. Everything except the pink layer

b. The “fluffy”-looking interphase
c. The pink lower phase (organic phase)

d. The clear upper phase (aqueous phase) X

]]> 2021-06-21 12:17:46 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1617530612 Group D) Why do we crush the fruit and why do we add shampoo? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1617562722 Answer:
crush the fruit: remove the protective layer of the fruit and get the liquid out.
add shampoo: the break the membrane and release the DNA. ]]> 2021-06-21 12:40:51 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1617562722 Group F) Why do we use ice-cold ethanol for gDNA extraction? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1617565387 answer: Cooling the mixture is to prevent the DNA from being degraded by the heating.
To form bigger precipitation "pieces"]]> 2021-06-21 12:42:36 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1617565387 Group A) What does the cold ethanol do during genomic DNA extraction? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1617567088 Answer: Due to the electrostatic attraction between the Na+ ions in
solution and the PO3– ions the DNA precipitates more easily]]> 2021-06-21 12:43:38 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1617567088 Group B) Why is it important to denature proteins during gDNA extraction? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618011742 Answer: Histones(proteins) create a dense DNA structure, which is not readily accessible. So to be able to get to the base pairs, we need to destroy that structure(->denaturize histones). Also, other proteins could interfere with our experimental procedure, so it's better to deactivate them.]]> 2021-06-21 16:55:07 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618011742 Group C) What happens in the denaturation step of PCR? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618021285 a. The DNA nucleotides are broken apart.
b. The base-pairing rules for DNA are reversed.
c. The double-stranded DNA is separated into two single strands of DNA. X
d. The DNA is returned to its natural setting. ]]> 2021-06-21 17:01:15 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618021285 Group D) What is the function of a primer? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618023539 a. To identify the particular region of DNA to be copied by PCR. X
b. To copy DNA.
c. To create DNA nucleotides.
d. To maintain the temperature of the PCR reaction.]]> 2021-06-21 17:02:44 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618023539 Group E) Why does PCR require a specific type of polymerase? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618027931 answer: It has to be heat resistant/stable. ]]> 2021-06-21 17:05:36 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618027931 Group F) How many cycles of PCR does it take to produce a double-stranded DNA fragment of the desired length? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618030556 a. 30-35 X
b. 1
c. 10
d. it depends on the length of the DNA fragment of interest]]> 2021-06-21 17:07:09 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618030556 Group A) Primer used for the process of polymerase chain reaction are ______________ marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618035885 a. Single stranded DNA oligonucleotide X
b. Double stranded DNA oligonucleotide
c. Single stranded RNA oligonucleotide
d. Double stranded RNA oligonucleotide]]> 2021-06-21 17:10:38 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618035885 Group B) Which of the following is a distinct advantage of the solid phase isolation technique? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618061636 a. Less expensive
b. More rapid and effective X
c. Doesn't require columns or beads
d. Doesn't require cell lysis]]> 2021-06-21 17:27:40 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618061636 Group C) Put the following types of naturally occurring RNA in order according to their abundance in cellular or total RNA. (First listed is most abundant) marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618065208 a. tRNA, mRNA, rRNA
b. mRNA, rRNA, tRNA
c. rRNA, tRNA, mRNA X
d. tRNA, rRNA, mRNA]]> 2021-06-21 17:30:14 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618065208 Group D) During the organic extraction technique in which genetic material is isolated from other cellular components, which layer is the nucleic acid found after phase separation? phase marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618067811 a. Interface phase
b. Lowermost phase
c. Uppermost phase X
d. Solid phase]]> 2021-06-21 17:32:05 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1618067811 Group E) Which type of phenol:chloroform:isoamyl alcohol do you need to use to isolate RNA? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1620300310 25:24:1]]> 2021-06-22 19:57:37 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1620300310 Group F) Why is DNA a polar molecule? marinazoppo8 https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1620305288 The phosphate group of the backbone is negatively charged. ]]> 2021-06-22 20:01:30 UTC https://padlet.com/olgalamprecht/acm0vf2nun3ik4gp/wish/1620305288