liver lab report by Abigail Champlin https://padlet.com/abigailchamplin/9xvn0c87kevi Liver enzymes break down peroxide en-us 2016-09-21 20:51:38 UTC 2025-10-05 22:25:57 UTC hello@padlet.com https://padlet-assets.s3.amazonaws.com/icons/Folder.png abigailchamplin https://padlet.com/abigailchamplin/9xvn0c87kevi/wish/125563738 Abby Champlin
Liver Enzyme lab

I.  Problem Every cell in your body produces peroxide when going through a chemical reaction but unfortunately it is poisonous. 

II. Hypothesis Prove that the liver produces an enzymes that breaks down poisonous peroxide in our bodies by converting it to oxygen and water.

III. Equipment and procedures
Equipment:
  • Water
  • Peroxide
  • 4 test tubes
  • Wooden splint
  • Matches
  • Tweezers
  • Pippets
  • Berkers
  • Liver
  • Bunsen burner
  • Test tube holder



 Procedures:
  1. To start the lab, make the first test tube a control, do this by using a pipette, fill it to the 2cm mark with water. Then add a piece of liver to the same test tube, record the reaction. 
  2. Next, fill the second test tube with peroxide to the 2cm mark, then add a piece of liver, record the reaction.
  3. Now fill the third test tube with 2cm of peroxide. Next take a wooden splint and light the end on fire with a match. Let it burn for a few seconds then blow it out, it should be glowing red, and slowly place into the tube, record reaction. 
  4. Now put 2cm peroxide in the fourth test tube, add the liver into the tube. Then light another splint on fire and slowly put down into the bubbles.  Record the reaction. 
  5. Next you will take a fifth test tube add 2cm of water and a piece of liver. Use the test tube holder to hold the test tube above the flame of the bunsen burner. Let this boil for a while. Now you will take the test tube and add peroxide. Record the reaction. 

IV. Observations 
A.Quantitative Observations none
B. Qualitative Observations

 Started the lab by making the first test tube a control, did this by using a pipette and filling it to the 2cm mark with water. Then added a piece of liver to the same test tube, and there was no reaction. Then the second test tube was filled with peroxide to the 2cm mark, and a piece of liver was added, there was a defiant chemical reaction. The third test tube was then filled with 2cm of peroxide. A wooden splint was set on fire with a match. Waited for a few seconds till it was glowing red then put it out. At this time it was then slowly place into the tube, and there was no reaction. The fourth test tube was now filled with 2cm peroxide, add the liver placed into the tube. There is a visual chemical reaction and another splint is set on fire then slowly put down into the bubbles. The splint is reignited when it comes into contact with the bubbles that were released. Then the fifth test tube is filled with 2cm of water and a piece of liver. Using the test tube holders, the test tube containing the liver is held above the flame of the bunsen burner. It is brought to a boil then is left for a while. Then the test tube is placed back into the rack where 2cm of peroxide is added. There is no reaction. 



V. Analysis
                                              A.Calculations none 
                                              B. Graphs


 | Water & liver | Peroxide & liver | Peroxide & flame | Peroxide, liver and flame.  | Boiled liver & peroxide.
Chemical Reaction | No reaction  | Yes reaction | No reaction  | Yes reaction | No reaction
Reaction to flame | N/A | N/A | Was not flammable. | Was Flammable | N/A
Proof of release of oxygen | No | No | No | Yes | No 


                        C. Questions


  • What are enzymes? An enzyme is a substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction.What are their roles in chemical reactions? Enzymes are proteins that serve as the function of speeding up a chemical reactions. Enzymes lower the amount of activation energy needed for a particular chemical reaction to occur. Without enzymes, many chemical reactions would occur at a much slower rate because the amount of activation energy required would be far greater.
  • Describe what happened to test tube 2 when hydrogen peroxide was added to the liver.  What gas was produced?  What liquid was left behind? The enzymes in the liver breaks down the peroxide converting it into oxygen and water. The gas being produced is oxygen and the water is left behind.
  • What effect does the red hot wood have on test tube 4? WHY is the flame being produced?  When the wooden splint was placed into test tube 4 the flame reignited. This occurred because the enzymes in the liver converted the peroxide into water and oxygen causing the splint to reignite,  oxygen is highly flammable. 
  • What effect does temperature have on the enzymes (Test tube 5)? When the liver is heated above optimal temperature this can cause a denaturing of the enzymes and therefore no chemical reaction will occur when the liver is placed into a solution of peroxide.



VI. Conclusion


I accept my hypothesis, I believe it to be true because it was proven by the data. My hypothesis says that the liver enzymes will break down the peroxide into water and oxygen. The data proves my hypothesis true,because the reactions showed when liver is added to peroxide, it's enzymes will break down the peroxide into oxygen and water.
 I have also learned that pure oxygen is highly flammable, and now know  how dangerous it can be to have any source of flame oxygen.
 An error that could occurred would be if the liver was diseased in a way causing the enzymes in the liver to become denatured before testing, causing there to be no reaction. 


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