https://padlet.com/nishadesai/9qv24zuwk0ty Made with whimsy en-us 2018-09-18 19:24:08 UTC 2025-07-26 16:29:20 UTC hello@padlet.com nishadesai https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287365397 2018-09-30 18:48:33 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287365397 nishadesai https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287366225 2018-09-30 18:52:23 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287366225 rennythai51 https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287366394 2018-09-30 18:53:27 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287366394 nishadesai https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287367221 2018-09-30 18:58:59 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287367221 nishadesai https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287367440 2018-09-30 19:00:24 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287367440 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287368955 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4479053/]]> 2018-09-30 19:09:03 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287368955 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287370426 https://www.ncbi.nlm.nih.gov/pubmed/24847416]]> 2018-09-30 19:17:58 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287370426 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287371621 https://link.springer.com/article/10.1007%2Fs00253-016-7692-4]]> 2018-09-30 19:25:17 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287371621 nishadesai https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287371793 https://waset.org/publications/10006897/natural-preservatives-an-alternative-for-chemical-preservative-used-in-foods]]> 2018-09-30 19:26:24 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287371793 nishadesai https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287372213 https://www.nature.com/news/food-preservatives-linked-to-obesity-and-gut-disease-1.16984]]> 2018-09-30 19:29:05 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287372213 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287372435 https://www.mdpi.com/1420-3049/17/12/14418]]> 2018-09-30 19:30:16 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287372435 nishadesai https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287374310 https://www.sciencedaily.com/releases/2017/08/170809155736.htm
https://www.nature.com/articles/s41467-017-00254-8]]> 2018-09-30 19:41:51 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287374310 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287374408 https://www.ncbi.nlm.nih.gov/pubmed/21535846]]> 2018-09-30 19:42:27 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287374408 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287375754 https://www.agrojournal.org/15/04-05-09.pdf]]> 2018-09-30 19:50:38 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287375754 rennythai51 https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287379418 https://ac.els-cdn.com/S0953756206002966/1-s2.0-S0953756206002966-main.pdf?_tid=caeb9f9c-bdea-4100-a430-45cd9de1895d&acdnat=1538338443_5f96aca26ec2f5b69aaf1456897586f1]]> 2018-09-30 20:17:50 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287379418 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287382834 http://tolweb.org/treehouses/?treehouse_id=4817]]> 2018-09-30 20:44:49 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287382834 nishadesai https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287384208 http://www.ijsrp.org/research-paper-0415/ijsrp-p4014.pdf]]> 2018-09-30 20:56:11 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287384208 rennythai51 https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287387806 https://www.britannica.com/science/Rhizopus]]> 2018-09-30 21:27:04 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287387806 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287421811 https://www.sciencedirect.com/science/article/pii/B9781845696696500157]]> 2018-10-01 02:50:53 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/287421811 nishadesai https://padlet.com/nishadesai/9qv24zuwk0ty/wish/288350223 2018-10-02 19:32:13 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/288350223 rennythai51 https://padlet.com/nishadesai/9qv24zuwk0ty/wish/288357159 https://docs.google.com/presentation/d/1g42vo88yKYomCs1KxKKIRBLzOfM18xCfqUKuPG5m4wI/edit?usp=sharing]]> 2018-10-02 19:46:37 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/288357159 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/288358103 https://www.fda.gov/food/ingredientspackaginglabeling/foodadditivesingredients/ucm091048.htm]]> 2018-10-02 19:48:49 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/288358103 rennythai51 https://padlet.com/nishadesai/9qv24zuwk0ty/wish/289974131 https://www.sciencedirect.com/science/article/pii/S000326700701999X]]> 2018-10-07 03:14:40 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/289974131 rennythai51 https://padlet.com/nishadesai/9qv24zuwk0ty/wish/289974149 https://www.sciencedirect.com/science/article/pii/S1466856407000045]]> 2018-10-07 03:15:12 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/289974149 rennythai51 https://padlet.com/nishadesai/9qv24zuwk0ty/wish/289974168 https://www.sciencedirect.com/science/article/pii/0168160592900352]]> 2018-10-07 03:15:46 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/289974168 rennythai51 https://padlet.com/nishadesai/9qv24zuwk0ty/wish/289974219 https://www.jstor.org/stable/43386906?casa_token=W5WxXzqkTx4AAAAA:XYeRPqj1KFiXAy5d-eFglYbbSFu1KPkOFmTQuLMj4taN6llaPFvY57uuZr3SmOkCrfJsd-epQNzIwzlHV_XFLFqhhAMc2nWVGaxYH-OGMu_GvRrStNA&seq=1#metadata_info_tab_contents]]> 2018-10-07 03:16:49 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/289974219 rennythai51 https://padlet.com/nishadesai/9qv24zuwk0ty/wish/289974242 https://www.sciencedirect.com/science/article/pii/S0168160513004741]]> 2018-10-07 03:17:15 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/289974242 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/290037846 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4221839/]]> 2018-10-07 15:16:18 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/290037846 nishadesai https://padlet.com/nishadesai/9qv24zuwk0ty/wish/290085122 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4289147/]]> 2018-10-07 21:00:45 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/290085122 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/290113888 https://microbiologyinfo.com/potato-dextrose-agar-pda-principle-uses-composition-procedure-and-colony-characteristics/]]> 2018-10-08 01:42:34 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/290113888 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/290539989 https://jcm.asm.org/content/48/10/3750]]> 2018-10-09 02:10:01 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/290539989 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/298591450 We made the tea tree, garlic extract, and the sodium benzoate solutions necessary for the pre-test. 
-High & low tea tree solution
-High and Low garlic extract solution
-0.05% sodium benzoate solution]]> 2018-10-30 16:02:21 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/298591450 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/298592230 We made liquid cultures of the Rhizopus stolonifer by taking loopful of spores/hyphae from original tube and mixing in potato dextrose media. (8 tubes total). We then incubated the cultures in a warm, 25C room temperature environment (in a box) for the fungi to grow. ]]> 2018-10-30 16:03:33 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/298592230 nishadesai https://padlet.com/nishadesai/9qv24zuwk0ty/wish/298649916 We took the tubes out of the incubator.  We wiped the condensation off 4 plates and then set them aside.  We turned the flame on and sterilized the spreader.  We micropipetted 200 microliters of liquid culture from the green-capped tube onto each of the four plates, leaving the lids open on the plates around the flame so that the liquid could evaporate and for sterile purposes.  We placed the disks on one plate and micropipetted 10 microliters of sodium benzoate on 4 discs, 10 microliters of low concentration tea tree oil on one disc, 10 microliters of high concentration tea tree oil on one disc, 10 microliters of low concentration garlic extract on one disc, and 10 microliters of high concentration garlic extract on another disc.  Then we used a micropipette to transfer these discs to their respective plates]]> 2018-10-30 17:32:37 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/298649916 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/301164640 We returned to lab the next day and checked plates for fungal growth. We were going to measure and record zones of inhibition around each disk, but none of the disks had a clear zone of inhibition and the fungi grew unevenly (some parts of the plate had no fungi and some did.) This may have been from our sources of error.
Possible Sources of Error: we may have not allowed the inoculating loop and spreader to cool for long enough, so it was too hot when we were spreading the fungal loop, and it may have killed the fungi. Also, we may not have spread the fungal solution evenly across plate.   ]]> 2018-11-06 18:50:34 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/301164640 rennythai51 https://padlet.com/nishadesai/9qv24zuwk0ty/wish/301178126 2018-11-06 19:10:52 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/301178126 rennythai51 https://padlet.com/nishadesai/9qv24zuwk0ty/wish/301178333 2018-11-06 19:11:09 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/301178333 rennythai51 https://padlet.com/nishadesai/9qv24zuwk0ty/wish/301178475 2018-11-06 19:11:21 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/301178475 rennythai51 https://padlet.com/nishadesai/9qv24zuwk0ty/wish/301178639 2018-11-06 19:11:34 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/301178639 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/303342169 We redid our pre-test and increased the concentrations. For sodium benzoate, we increased it to 5 grams in 10 mL. For plant-based preservatives, we did concentrations of 25%, 50%, and 100%. We repeated disk diffusion assay.]]> 2018-11-12 16:26:20 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/303342169 nishadesai https://padlet.com/nishadesai/9qv24zuwk0ty/wish/303342429 There was no growth of Rhizopus stolonifer.  There were a few contaminants on the plates.  A possible source of error is that we used a different tube than the first trial.  The tube that we used might have not had enough R. stolonifer cells in the liquid. ]]> 2018-11-12 16:26:49 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/303342429 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/303346618 We stored the plates in an 30 degree Celsius incubator instead of the box, and even though Rhizopus stolonifer can grow at 30 degrees Celsius, it may not have been ideal for growth. Also, maybe our concentrations of preservatives were too high, and it killed all the fungi, so there was no growth to measure.]]> 2018-11-12 16:34:19 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/303346618 rheasudhakaran https://padlet.com/nishadesai/9qv24zuwk0ty/wish/303349803 1. Original Method (spread preservative solutions over surface of plate, put Rhizopus stolonifer solid samples in center, record surface area and/or height)
2. Agar Well Diffusion Assay (similar to disk, except bore wells into agar and put solutions into wells and record zones of inhibition)]]> 2018-11-12 16:39:51 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/303349803 nishadesai https://padlet.com/nishadesai/9qv24zuwk0ty/wish/304029637 We redid our pretest and used concentrations of 25%, 50%, and 100% for the plant-based preservatives.  We used 25%, 50% and 0.1% (industry standard) sodium benzoate.  We repeated disk diffusion assay.

]]> 2018-11-13 20:50:17 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/304029637 rennythai51 https://padlet.com/nishadesai/9qv24zuwk0ty/wish/304030749 Since the fungi did not grow at all on any of the plates during our second trial, we thought the concentration of the fungi we put on the plates were weak. As a result, we combined the original 8 culture tubes into 3 tubes so our cultures could be more concentrated. We then incubated the 3 culture tubes at 30C.]]> 2018-11-13 20:53:17 UTC https://padlet.com/nishadesai/9qv24zuwk0ty/wish/304030749