D1-Team 1 by Sarah Ng https://padlet.com/Sarahhhhhh9/9dd94h9edwyng59h Disposable VS Fixed-in-place bioreactor en-us 2023-12-04 04:55:57 UTC 2023-12-04 12:00:04 UTC hello@padlet.com sarah Sarahhhhhh9 https://padlet.com/Sarahhhhhh9/9dd94h9edwyng59h/wish/2812353678 In theory, both bioreactors have their advantages and disadvantages to justify why they are better in certain aspects and circumstances. However, I would suggest that disposable bioreactors are better, especially for research, biopharmaceutical production and HTS.

Disposable bioreactors are more often preferred than Fixed-in-place stainless steel bioreactors for smaller-scale production because they are more flexible, allowing frequent variability in the production volume, or the cell line used, etc.; which would be more suited to the ever-evolving research and process needs as well as development.

Furthermore, disposable bioreactors have faster turnaround time between batches so it eliminates their need for expensive and intrinsic automation to clean and sterilize the bioreactor; Clean-in-place and sterilize-in-place procedures.

Moreover, disposable systems have reduced the risk of cross-contamination between batches since they use single-use components and the bioreactor is sterilized before each batch run. This would, therefore, prevent the introduction of any unwanted microorganisms in the product and ensure quality and safety. Even now as it stands, there has been a steadily growing trend in the biopharmaceutical industries using disposable systems for biologics production for personalized medicine.

]]> 2023-12-04 05:01:42 UTC https://padlet.com/Sarahhhhhh9/9dd94h9edwyng59h/wish/2812353678 Fixed in place (Gyann) chiagyann https://padlet.com/Sarahhhhhh9/9dd94h9edwyng59h/wish/2812463672

  • High utility cost

  • Leakage from piping tubes

  • Difficulties in cleaning and maintenance, require regular maintenance to ensure proper functioning.

  • Despite rigorous cleaning procedures, there is always a risk of cross-contamination between different batches or products, especially if the bioreactor is used for multiple processes.

  • Designed for specific volumes and processes. They may not be easily adaptable to changes in scale or modifications to the bioprocess.


    • ]]>     2023-12-04 07:10:28 UTC https://padlet.com/Sarahhhhhh9/9dd94h9edwyng59h/wish/2812463672     Disposable bioreactors (brindha) https://padlet.com/Sarahhhhhh9/9dd94h9edwyng59h/wish/2812529936

  • product limitation

    - not always suitable for large scale manufacture

    - limitation in liquid transfer

    - temperature and pressure sensitivity

    - damage to single-use bags (puncture) can impose leakage

  • requires ongoing consumables usage forecast

  • quality by design needs to consider extractables and leachable studies' data

  • relatively new technology - workforce skills gap

  • places more responsibility on the operators (quality control and cleaning)

  • expensive

    - slight increase in variable costs per run

    - disposable costs


    • references:

    https://youtu.be/eAtg47Fr4XU?si=TkUBRvZIUlZm4BaY

    https://www.pharmtech.com/view/pros-and-cons-single-use-bioreactors

    ]]>     2023-12-04 08:23:10 UTC https://padlet.com/Sarahhhhhh9/9dd94h9edwyng59h/wish/2812529936     Disposable bioreactors (brindha) https://padlet.com/Sarahhhhhh9/9dd94h9edwyng59h/wish/2812609300

  • speed and ease of deployment

    - ease of installation, and moving

    - cleaning and validation requirements reduced

  • cleaning process is avoided

    - eliminates validation issues

    - shortens downtime/turnaround time

  • lowers risk of contamination

  • fast changeover resulting in greater batches

    - new bags for each run

  • reduced capital expenditure for equipment and facility

    - savings on start-up capital costa, utilities, space, labour and cleaning requirements

  • increase plant flexibility


    • references:

    https://youtu.be/eAtg47Fr4XU?si=TkUBRvZIUlZm4BaY

    https://www.pharmtech.com/view/pros-and-cons-single-use-bioreactors

    ]]>     2023-12-04 09:42:53 UTC https://padlet.com/Sarahhhhhh9/9dd94h9edwyng59h/wish/2812609300     Fixed-in-place bioreactors (aisyah) https://padlet.com/Sarahhhhhh9/9dd94h9edwyng59h/wish/2812739893

  • Scales up to 20,000L, which are larger capacities. Allows for higher generation of product.

  • More popular (used by more, is relatively old, so it is more widespread and more understood.)


    • Reference: https://www.cytivalifesciences.com/en/us/news-center/single-use-or-stainless-steel-bioreactors-10001

    ]]>     2023-12-04 11:50:51 UTC https://padlet.com/Sarahhhhhh9/9dd94h9edwyng59h/wish/2812739893