BLT Padlet by Prachi Stafford https://padlet.com/hwbps9/8g5apvqku4sk Made with panache en-us 2016-09-30 15:01:17 UTC 2024-05-26 14:43:31 UTC hello@padlet.com http://www.bu.edu/flow-cytometry/files/2010/10/BD-Flow-Cytom-Learning-Guide.pdf hwbps9 https://padlet.com/hwbps9/8g5apvqku4sk/wish/196469720
The above is a useful guide re: flow cytometry]]> 2017-10-12 15:02:33 UTC https://padlet.com/hwbps9/8g5apvqku4sk/wish/196469720 IHC in Lung Cancer https://padlet.com/hwbps9/8g5apvqku4sk/wish/292452714 Biomarkers are probe for ALK and ROS1 gene. Other techniques that can also be used to detect this two genes are: FISH and RT- PCR


Question from PS: What do you look at with IHC? Think in terms of genes & proteins...]]> 2018-10-13 19:03:52 UTC https://padlet.com/hwbps9/8g5apvqku4sk/wish/292452714 Use of IHC in Disease example (Muscular Disease) https://padlet.com/hwbps9/8g5apvqku4sk/wish/292587974 A recent example of the usefulness of IHC in diagnosing certain diseases has been shown with muscular diseases. Certain proteins including those found in the extracellular matrix, cytosol, nucleus of muscle fibres have been found to be abnormal in patients suffering with muscular dystrophies. IHC has been used to identify these specific abnormalities and therefore assist in the diagnostics, which includes distinguishing between vascular dystrophy and non-dystrophic disorders in skeletal muscles. However, it is only useful for the dystrophies that have these known abnormal proteins, as the technique needs to be targeted to these markers. 

Question from PS: Good example of application but can you give examples of specific markers?]]> 2018-10-14 20:18:46 UTC https://padlet.com/hwbps9/8g5apvqku4sk/wish/292587974 Video re: Western blotting hwbps9 https://padlet.com/hwbps9/8g5apvqku4sk/wish/294827310 https://youtu.be/uFu8aie4QFI]]> 2018-10-19 13:59:44 UTC https://padlet.com/hwbps9/8g5apvqku4sk/wish/294827310 Useful reading for IHC hwbps9 https://padlet.com/hwbps9/8g5apvqku4sk/wish/294827527 2018-10-19 14:00:11 UTC https://padlet.com/hwbps9/8g5apvqku4sk/wish/294827527 Video re: Haemagglutination assay: hwbps9 https://padlet.com/hwbps9/8g5apvqku4sk/wish/294828089 https://youtu.be/CFCi5Q4rhOU]]> 2018-10-19 14:01:18 UTC https://padlet.com/hwbps9/8g5apvqku4sk/wish/294828089 Hello, Welcome to the BLT padlet wall. Please ask your questions here. hwbps9 https://padlet.com/hwbps9/8g5apvqku4sk/wish/294828276 BW]]> 2018-10-19 14:01:39 UTC https://padlet.com/hwbps9/8g5apvqku4sk/wish/294828276 Uses of IHC https://padlet.com/hwbps9/8g5apvqku4sk/wish/295437838 IHC is commonly used in cancer diagnosis and prognosis. Markers can be used to suggest the suitability of a treatment option. 
Example in differentiating types of lung cancer could include biomarkers of p40, CK5/6 and TP63 which identify squamous cell carcinoma over non-squamous cell carcinoma. 
Biomarkers determine which therapies should be used in lung cancer include Alk, ROS1 and EGFR. 
(DOI: 10.3390/cancers10030072)]]> 2018-10-22 13:30:56 UTC https://padlet.com/hwbps9/8g5apvqku4sk/wish/295437838 Use of IHC https://padlet.com/hwbps9/8g5apvqku4sk/wish/297739621 Inmunohistochemistry can be used for the assesment of HER2 (human epidermal growth factor receptor 2) expression in human breast tumor cells in order to predict the response to Trastuzumab which is a monoclonal antibody. What is more, Trastuzumab targets the extracellular domain of HER2 receptor. HER2 is a member of the epidermal growth  factor receptor (EGFR) family of molecules and is encoded for by the HER2 proto-oncogene. It is showed that overexpression of HER2 receptors is present in 10-20% of primary breast cancer. Therefore, formalin-fixed paraffin sections immunostained are used to prove HER2 receptor overexpression. If those cells present HER2 overexpression, then the patient can be treated with Trastuzumab.

There are other alternative techniques which can be used for HER2 assessment such as FISH (fluorescence in situ hybridization) and CISH (Chromogenic in situ hydridization)

References 

Suvarna, S., Layton, C., & Bancroft, J. (2013). Bancroft’s theory and practice of histological techniques (7th ed.). Oxford: Churchill Livingstone.

Martin, A.P., Downing, J., Cochrane, M., Collins, B., Francis, B., Haycox, A., Alfirevic, A., Pirmohamed, M. (2018). Trastuzumab uptake in HER2-positive breast cancer patients: a systematic review and meta-analysis of observational studies. Critical Reviews in Oncology/Hematology, 130, 92-107.

]]> 2018-10-28 11:50:45 UTC https://padlet.com/hwbps9/8g5apvqku4sk/wish/297739621 Useful videos re: H&E for tissue samples hwbps9 https://padlet.com/hwbps9/8g5apvqku4sk/wish/301374229 Liver
https://youtu.be/KtFJvlEDE2I

Kidney
https://youtu.be/9Nh6RBK42qM

Lungs
https://youtu.be/U6LqEr4vVis

Spleen
https://youtu.be/Kl5L01BCYC8

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