Strawberry DNA Lab by Roselyn Cruz https://padlet.com/roselync102965/Bookmarks Made with a bold sensibility en-us 2022-03-16 15:42:43 UTC 2022-03-18 22:10:07 UTC hello@padlet.com https://padlet.net/icons/png/1f353.png SECTION 1: STRAWBERRY DNA EXTRACTION LAB roselync102965 https://padlet.com/roselync102965/Bookmarks/wish/2098359756 NAME: Roselyn
PERIOD: 2th Period
DATE: March/16/2022]]> 2022-03-16 16:03:34 UTC https://padlet.com/roselync102965/Bookmarks/wish/2098359756 SECTION 2: DNA INTRODUCTION PARAGRAPH roselync102965 https://padlet.com/roselync102965/Bookmarks/wish/2098425282 DNA stands for Deoxyribonucleic acid. DNA is made of nucleotides. A nucleotide is made of three group's the three groups being nitrogenous base, a pentose sugar, and a phosphate. The base pair rules of a DNA is (A)= Adenine (C)=Cytosine (G)= Guanine (T)= Thymine. They are healed  by a special chemical called hydrogen bonds. DNA looks like a guwy, slimy clear fluid. ]]> 2022-03-16 16:37:54 UTC https://padlet.com/roselync102965/Bookmarks/wish/2098425282 SECTION 4: MATERIALS & PICTURES roselync102965 https://padlet.com/roselync102965/Bookmarks/wish/2098431254
  • GOGGLES
  • Heavy duty Ziplock bag
  • 1 strawberry
  • 10 mL DNA extraction buffer (soapy, salty water)
  • Cheesecloth
  • Funnel
  • 20 mL test tube & test tube stand
  • Stir stick
  • 10 mL ethanol (FREEZER)
  • Paper towels

    • ]]>     2022-03-16 16:41:07 UTC https://padlet.com/roselync102965/Bookmarks/wish/2098431254     SECTION 5: PROCEDURES & PICTURES roselync102965 https://padlet.com/roselync102965/Bookmarks/wish/2098447175
  • Place one strawberry in a Ziploc bag.
  • Smash/grind up the strawberry using your fist & fingers for 2 minutes. Careful not to break the bag!!
  • Add the provided 10 mL of extraction buffer (salt & soap solution) to the bag. (DNA Extraction Buffer:1000 ml of deionized water, 50 ml of dish soap, 1 teaspoon of salt)
  • Gently kneed/mush the strawberry in the bag again for 1 minute.
  • Assemble your filtration apparatus as shown in the diagram.
  • Pour the strawberry slurry into the filtration apparatus & let it drip directly into your test tube … only until your test tube is ½ full.
  • Slowly pour cold ethanol into the tube. OBSERVE  😊
  • Dip the stir stick into the tube where the strawberry extract and ethanol layers come into contact with each other. OBSERVE  😊



    • ]]>     2022-03-16 16:49:47 UTC https://padlet.com/roselync102965/Bookmarks/wish/2098447175     SECTION 6: DATA ANALYSIS PARAGRAPH roselync102965 https://padlet.com/roselync102965/Bookmarks/wish/2098457196 First i put the strawberry in the zip lock bag we smashed the strawberry into a watery consistency in order to be able to to squish the DNA out the strawberry, After for the second step we added the solution which was the soup, water and salt solution. Then we continued to mix the mashed up strawberry with the solution. After mixing thuraly for a couple minutes me and my partner thought that it was ready to be pored into the cheesecloth and what the cheesecloth did was separate all the chunks of the strawberry from the juice, the juice fell into a beaker then was pored into a test tub which then the ethanol would be pored on top. After we waited for a couple seconds and began to see in between the two laters which were the strawberry juices mixed with our solution of water, soup and salt we were able to see a clear thick bubbly consistency forming we took the stir stick that was given to us and stuck it straight into our test tub without mixing the strawberry with the ethanol and took the stir stick out to see a clear slimy, guwy, sticky consistency of strawberry DNA.]]> 2022-03-16 16:55:18 UTC https://padlet.com/roselync102965/Bookmarks/wish/2098457196 SECTION 7: EXTENTION QUESTIONS roselync102965 https://padlet.com/roselync102965/Bookmarks/wish/2098488664 What we did for this lab was we did an experiment to extract the DNA of a strawberry. What i learned was that in one of the procedures we had to use alcohol and i learned that alcohol doesn't freeze and turn into a solid. What i would do differently is take more pictures. The next step after removing the DNA from A strawberry i believe would be sending it to a lab. Two examples of traits in A strawberry would be the sweetness of A strawberry and the color of a strawberry. ]]> 2022-03-16 17:13:05 UTC https://padlet.com/roselync102965/Bookmarks/wish/2098488664 SECTION 8: CLEAN UP PROCEDURES roselync102965 https://padlet.com/roselync102965/Bookmarks/wish/2101308340
  • Wash supplies with soap & water
  • Put supplies on towels to dry
  • TRASH:
    • Ziplock bag
    • Strawberry stem
    • Stick
    • cheesecloth
    1. Wipe lab tables with Lysol wipes
    2. Put Goggles away


    ]]>     2022-03-18 04:19:36 UTC https://padlet.com/roselync102965/Bookmarks/wish/2101308340     SECTION 3: DNA/RNA T-CHART roselync102965 https://padlet.com/roselync102965/Bookmarks/wish/2102549943
     ]]>     2022-03-18 21:52:35 UTC https://padlet.com/roselync102965/Bookmarks/wish/2102549943