Dialogue:

I think that this is a better way of changing gene editing
Summary:
Genome editing is a group of technologies that give scientists the ability to change an organism's DNA. The bacteria then use Cas9 or a similar enzyme to cut the DNA apart, which disables the virus.
Scientists are still working to determine whether this approach is safe and effective for use in people.
APA
What are genome editing and CRISPR-Cas9?: MedlinePlus Genetics. (n.d.). retrived from:
https://medlineplus.gov/genetics/understanding/genomicresearch/genomeediting/

Dialogue:

This article is very interesant if you like this topic
Summary:
Genome editing tools have the potential to help treat diseases with a genomic basis, like cystic fibrosis and diabetes. This therapy saved Layla's life. However, treatments like the one that Layla received are still experimental because the scientific community and policymakers still have to address technical barriers and ethical concerns surrounding genome editing.
APA: 
National human genome (2019, August 15) What is gene editing ?
Retrived from: https://www.genome.gov/about-genomics/policy-issues/what-is-Genome-Editing 

- Process

Dialogue:

I thing that is a good web or article but there are better articles of this topic because they don't explain a lot of thing, but a good thing that they do is that explains the history
Summary:
Genome editing, or genome engineering, or gene editing, is a type of genetic engineering in which DNA is inserted, deleted, modified or replaced in the genome of a living organism. Unlike early genetic engineering techniques that randomly inserts genetic material into a host genome, genome editing targets the insertions to site-specific locations. The basic mechanism involved in genetic manipulations through programmable nucleases is the recognition of target genomic loci and binding of effector DNA-binding domain , double-strand breaks in target DNA by the restriction endonucleases , and the repair of DSBs through homology-directed recombination or non-homologous end joining .
APA :
Wikipedia contributors,(2022, december, 11) Genome editing
retrived form: https://en.wikipedia.org/wiki/Genome_editing

Dialogue:

This article is not very essy to understand but, when you understand is a very good article.

Summary:
Gene editing is performed using enzymes, particularly nucleases that have been engineered to target a specific DNA sequence, where they introduce cuts into the DNA strands, enabling the removal of existing DNA and the insertion of replacement DNA. Key among gene-editing technologies is a molecular tool known as CRISPR-Cas9, a powerful technology discovered in 2012 by American scientist Jennifer Doudna, French scientist Emmanuelle Charpentier, and colleagues and refined by American scientist Feng Zhang and colleagues.

APA: Rogers,Kara . Fridovichfeli,Judith (2016, January 07)
Britannica 
retrived from: https://www.britannica.com/science/gene-editing

Dialogue: I think these is a better way of to try to tell us that gene editing is good and in these point of view gene editing is good.

Summary: Every guide, including the target and tracrRNA sequence, is produced using fully customized de novo synthesis.

APA
Unknown  retrevied (31/1/2023) Advance your research with Agilent gene editing solutions

Dialogue:

Making special tools for gene editing
Summary:
One groundbreaking area of focus is gene editing, a technique that allows scientists to, for example, correct faulty genes that cause a specific disease, thereby aiming to reverse certain disease symptoms or prevent the disease from occurring in the first place.
APA
Unknown (2020,5 noviembre) What is gene editing  https://www.bayer.com/en/news-stories/what-is-gene-editing

Dialogue:

Why theres so many variants of covid 19?
Summary:
CrisprBits, one of the first Indian companies using CRISPR, a breakthrough gene-editing technology, on Tuesday announced that it has developed OmiCrisp, a CRISPR- based test to detect SARS-CoV2. The comany claims that the test can also determine whether it is an Omicron or non-omicron variant. « The patent pending OmiCrisp test uses the Cas12a enzyme and separate guides to target variant-specific sequences found in Omicron and non-Omicron variants of SARS-CoV-2,» company stated on Tuesday. «The test was tested on over 80 clinical samples and was 100 pecent accurate in identifying whether a sample was Omicron or non-Omicron when compared to sequencing results.
APA
Health desk(January 24)CrisprBits develops gene-editing technology-based test to detect Omicron variant 
retrived from:
https://www.financialexpress.com/healthcare/covid-19/crisprbits-develops-gene-editing-technology-based-test-to-detect-omicron-variant/2958618/

Dialogue: In my opinion I think these is not a good idea because these will give as a lot of problems

Summary: Bassicly they are trying to control as by changing are DNA and win money.

APA
Harvard (January 9, 2019) Perspective of gene editing.

Dialogue:

How do they treat so clean desease
Summary:
From large-scale screening to focused gene editing, CRISPR is a powerful genome editing technology. Specificity and stability are paramount to the successful implementation of guide RNA , as off-target edits can have significant impacts in downstream applications.
The successful use of DNA oligo pools for CRISPR screening relies upon the fidelity and representation of the starting library.
APA
Advance your research with Agilent CRISPR solutions | Agilent. (n.d.). retrived from:
https://www.agilent.com/en/solutions/genomics-applications-solutions/crispr?ds_rl=1299916

Dialogue: I didn't now that this topic was so interesant

Summary:
In just 10 years, CRISPR has revolutionized gene editing. The technique has fueled a wave of new scientific discoveries in cancer, Alzheimer’s, and heart disease, and several gene therapies that deploy the gene editor will soon be available to patients.

APA:  Hoffmann . Florian  et al (2022, August 24)The Next Revolution in Gene Editing?
Retrived from : https://www.cuimc.columbia.edu/news/next-revolution-gene-editing

Dialogue:

This is one of the best articles because explain the best points of positive and negative
Summary:
The primary debate around gene editing is whether or not it’s safe to modify human DNA and genetic sequences. Some experts believe that current technology is sufficient, and embryos could greatly benefit from being altered.
Since these consequences are avoidable, they insist that any potential rewards are not worth the risk.
Another common argument stems from the perceived inevitability of non-therapeutic gene editing and enhancement procedures.
APA:
Rex Md (2022, May 18) What Is Gene Editing? 
Is It Moral or Ethical? Retrieved from
https://rexmd.com/learn/what-is-gene-editing

- Genome Editing and NIH Funding

Dialogue:

They are making clinics of gene editing.
Summary:
Genome editing can be used to correct, introduce or delete almost any DNA sequence in many different types of cells and organisms. While techniques to modify DNA have existed for several decades, new methods have made genome editing faster, cheaper and more efficient. Genome editing builds on an earlier discovery that a broken section of DNA in a gene triggers a cell’s repair mechanism to stitch together the break. Genome editing allows researchers to mimic this natural process of DNA repair.

Advanced genome editing methods engineered from proteins include zinc-finger nucleases , transcription activator-like effector nucleases , and meganucleases. CRISPR/Cas9 is the most widely used genome editor and is a powerful tool for understanding gene function.
APA
Gene Editing â Digital Media Kit. (2020, 5 noviembre). National Institutes of Health (NIH). 

Dialogue: 
Interventions of bad use of gene editing.

Summary: Genome editing is a method for making specific changes to the DNA of a cell or organism. It can be used to add, remove or alter DNA in the genome. Application of somatic human genome editing has already been undertaken, including in vivo editing, to address HIV and sickle-cell disease, for example. Although somatic human genome editing is well established and acceptable for treatments in some scientifically advanced countries with regulations in place, challenges remain. Other challenges are associated with rogue clinics, medical travel, as well as the reporting of illegal, unregistered, unethical or unsafe research and other activities including the offer of unproven so-called therapeutic interventions.
Heritable human genome editing refers to editing of nuclear DNA in a way that may be heritable across generations.

APA
Human genome editing. (2021, 20 octubre).

Dialogue: Experimenting with kids is not a good thing bud they offer them self to doit thats why is not a bud thing.

Summary:
The paper, published by David Liu’s lab at the Broad Institute of MIT and Harvard, described a version of Crispr gene editing that allowed for more precise changes than ever before. «It seemed like science fiction had arrived,» says Qasim, who teaches at University College London.
APA
Mullin, E. (2022, 23 diciembre). A More Elegant Form of Gene Editing Progresses to Human Testing. Retrived from https://www.wired.com/story/a-more-elegant-form-of-gene-editing-progresses-to-human-testing/

Dialogue:

Why do parents allow their kids to talk with strangers
Summary:
This web page talks about a conversation about kids talking online with other people.The strange people talks with the kids because they just want to hack
APA

Sneed, A. (2017, November 2). Mail-Order CRISPR Kits Allow Absolutely Anyone to Hack DNA. Scientific American. retrived from:https://www.scientificamerican.com/article/mail-order-crispr-kits-allow-absolutely-anyone-to-hack-dna/

Dialogue:

These will be a better way of changing DNA
Summary:
Advances in gene editing, and CRISPR technology in particular, may soon make it possible. In the early days, CRISPR was used to simply make cuts in DNA. Today, it’s being tested as a way to change existing genetic code, even by inserting all-new chunks of DNA or possibly entire genes into someone’s genome. «Even if you start with a normal cholesterol level, and you turn off PCSK9 and bring cholesterol levels even lower, that reduces the risk of having a heart attack,» says Musunuru.
APA
Hamzelou, J. (2023, 20 enero). Next up for CRISPR: Gene editing for the masses? MIT Technology Review.

Dialogue:

How do people change genome
Summary:
Genome editing is a way of making changes to specific parts of a genome. Dozens of countries already prohibit any attempt to start a pregnancy with edited embryos, yet some scientists seem eager to proceed.
Any discussion of how we might use this technology in the future needs to consider the serious societal consequences of human genome editing.
APA

Center for genetics and society(n.d.)fetrived from:https://www.geneticsandsociety.org/internal-content/what-human-gene-editing

Dialogue: Thermo editing with humans

Summary:
To help researchers in their quest to understand how the genome influences phenotype, we’ve developed a complete set of trusted solutions for every step in the gene editing workflow. Because every lab is unique, we offer a variety of genome editing solutions to meet your needs. Whether you want results fast, seek full control over every design step, or need help engineering cells to your specific requirements, we have solutions that fit.
APA
Unknown(2020,5 december) Thermo fisher https://www.thermofisher.com/es/es/home/life-science/genome-editing.html

Dialogue:

Changing cell info
Summary:
Gene editing refers to the process of changing regions of cellular DNA. These changes are permanent and heritable, resulting in a newly engineered cell line.
Additionally, Horizon offers a wide selection of pre-made edited cell lines and expert genome engineering services.
APA
Gene editing