My Phenomenal Padlet by Balqis Mustazim

My First Reflective Journal

BalqisMustazim — 2016-09-13 05:04:42 UTC

Assalamualaikum and hye! For my first reflective journal, I would like to talk about my first impression about Microbiology. I was exposed to this course when I came to Faculty of Biotech's booth at edufair last March which is organized at UPM Serdang. I think this course is very interesting when it come to occupation sectors. Moreover, I really amazed with the specific name of the microbes and also their harmful and benefits to us. As I attended my first Microbiology 1 with Dr Wan, I never doubt my first expectation about this course. Dr Wan really make me more curious and increased my spirit to explore more. I hope, I will get along with this course till the end. I will try my best to pass with flying colours even I'm the only one from this course that stay in KOSASS.

My Second Entry

BalqisMustazim — 2016-09-19 02:07:15 UTC

On Thursday, 14092016, I had learnt about the different between procaryotes which are only Bacteria and Archaea and eucaryotes which are the other microbes. What eucaryotes have but procaryotes don't have are, nucleus, mitochondria, golgi apparatus and endoplasmic reticulum. Besides, on that day, my group and I had to discuss about archaea. Archaea is a single-celled microbes. Its cell wall lack peptidoglycan(polymer consists of sugars and amino acids that form outside the plasma membrane) but they have pseudopeptidoglycon. Meanwhile, archaea can survive in extreme conditions(high temperature, high pressure, low pH) as they have more stable stable membrane chemistry. Its amazing right?! . I really enjoy the class with Dr Wan very much as the group formed make we cooperate with each other to search more about the microbes that we had learn and share the information to the class. We also can know either the information that we searched true or not as the website maybe mistype or stated the wrong facts about the microbes.

Presentation on Microscopy

BalqisMustazim — 2016-09-27 08:33:18 UTC

I would like to share some knowledge that my group members and I had present. I'm one of the member from group 7. We presented on the topic The Fluorescence Microscopy. Here, I would like to share a video that explain more details about the fluorescence microscopy. It usually use in medical microbiology and microbial ecology studies. Hope you'll enjoy the video and understand more about the this microscopy

History of Vaccination

BalqisMustazim — 2016-10-05 00:18:19 UTC

In last lecture, I had find some information about the history of vaccination which is about Edward Jenner that inoculated his own son to discovered the vaccine for swinepox. Here, I'll share about it. In 1789, Jenner decided (just after he had been elected as a Fellow of the Royal Society) to try immunizing (or what he thought was immunizing) his ten month old son, Edward, Jr, and two of his neighbor’s servants, by inoculating them with swinepox.

He had learned well from his famous teacher, Dr John Hunter, that one will learn more by “trying the experiment” rather than by just speculating about it. So Jenner performed the experiment by making a small scratch on the servants’ and the baby’s arms with a lancet and then infecting the scratch “with matter from a pustule of the baby’s nurse, who had caught the swinepox infection.” Eight days later baby Edward took sick and developed sores, but he did eventually recover.

Then, two years later, Jenner again challenged his son with smallpox again, this time, with unhappy results. This time there was a reaction, and a severe one. But he quickly recovered, and a year later Jenner inoculated him with smallpox once again.

Unfortunately, however, in the years following these experiments, young Edward “became a sickly child and exhibited signs of mild mental retardation,” likely due to neurological damage.

Jenner’s son died at age 21 from tuberculosis, which has been linked to the smallpox vaccine. Despite his death, Jenner continued testing his vaccine on many others producing varying results such as vaccine-induced smallpox and vaccine-induced tuberculosis.

Jenner, in 1798, formulated a new vaccine, which combined “horse-grease” and cow-pox matter as he had proclaimed his first formulation in 1796 as having “no protective virtue.” His new vaccine was met with public disdain and disgust and his experiments failed. He was ridiculed. Jenner then returned to promote his original formulation.

By 1807, he convinced the Royal College of Physicians and the British Parliament that his once defunct and admittedly unprotective vaccine was safe and effective, and as well could produce large revenues. Not trusting the scientific solutions being offered, some countries banned variolation. Yet, quite surprisingly, Jenner’s vaccine became compulsory and mandatory in many other countries. The smallpox vaccine was widely used until 1979 when the World Health Organization (WHO) declared smallpox eradicated from the face of the earth.

Virtual Lab

BalqisMustazim — 2016-12-17 05:34:08 UTC

Virtual lab is a very interesting website to learn as I became more understand about this gram staining.

Gram staining used to view cellular morphology and for diagnostic purpose which is about what type of cell wall a bacterium has.

Gram positive = thicker peptidoglycan layer and no outer membrane
Gram negative = thinner peptidoglycan and an outer membrane.

Reagents used for the staining are crystal violet (purple), iodine, ethanol, safranin (pink).

Function of the reagents :
- crystal violet used as primary stain ; +ve stain and it stains the cell wall purple
- iodine used as mordant and it combines with crystal violet to form an insoluble complex that gets trapped in the thicker peptidoglycan layers. If forget, the ethanol will decolorized the gram positive cells`
- ethanol used as decolorizer. If forget, everything will have crystal violet on it either gram positive or negative.
- safranin used as counterstain. It is a simple +ve stain that provides dye for decolorized cells for gram -ve. It stain all cells, but only -ve ones actually appear pink. If forget, we difficult to visualize and identify gram -ve organism and their thin cells may have bound enogh through crystal violet and will misidentified with gram +ve.

The Project

BalqisMustazim — 2016-12-17 09:01:45 UTC

Sept 23, 2016 @DKU

Our first microbiology conference with other university. Definitely, I'm sooooo excited. The purpose of the conference is about out MEGA project which was the Virtual Microbes. The project in collabs, UPM-UM-UKM-UMT . We definitely met our other friends from the Microbes World!

And, this picture is definitely us with that excited (kind of jakun actually lol) faces. Great time ahead looking with this cute aliens.

MICROSCOPE

BalqisMustazim — 2016-12-17 09:20:14 UTC

For thhis entry, I would like to share the infographic about Microscope.

VM1-G1-T20

BalqisMustazim — 2016-12-17 09:56:51 UTC

I'm one of the member from team 20. Our adopted microbe is Lactobacillus Bulgaricus. This bacteria is a gram positive bacteria. The reason I suggested this bacteria to my group members as one day when I ate a yogurt, I've an instinct to take a look at the ingredients part of the marigold yogurt since before I didn't notice if there is a part that write about what the microbes used to ferment the yogurt at the yogurt's container. The other members agreed to adopt this microbe as they said, "Why not we adopt this Lactobacillus Bulgaricus as we all love yogurt".

Lactobacillus Bulgaricus probiotics and functional foods are made in Bulgaria. This video is about the technology of preparation, the team, the equipment, the list of products: foods, probiotics, synbiotics, cosmetics.

Progress of the Project

BalqisMustazim — 2016-12-17 10:16:38 UTC

It quite difficult to have the next streaming video as the members were busy with their university activity as they also on their week test. But, Alhamdulillah we managed to do the project well. All of the member cooperate to make sure the things finished and we enjoyed it as well.

The Last Streaming

BalqisMustazim — 2016-12-17 10:19:54 UTC

This time our streaming is quite special as Choy was also there hehe. Choy is a very kind person and he guide us well to make sure we're not feel burden with this project. Thank you Choy! This is our last video conference. A bit sorry as my lappy gone wrong at the end of the video.

Algal Bloom

BalqisMustazim — 2016-12-17 10:33:02 UTC

We had our class in computer lab for this topic, Protist. We discuss about this topic in the group and divide the parts to the note for each group members. I did the part Pyrrophyta.

Pyrrophyta or known as Dinoflagellate. The single-celled dinoflagellates are bioluminescent. When conditions are right, dinoflagellates bloom in dense layers at the surface of the water, causing the ocean to take on reddish-brown color in daylight and a sparkly sheen as they move in the wave at night. When they're eaten, the toxic dinoflagellates accumulate in high concentrations in larger fish and filter feeding shellfish. And when marine mammals or people eat these organisms, it can cause sickness or even death.

Here, I share a website about the news of Algal Bloom.

The Toadstool Mushroom

BalqisMustazim — 2016-12-17 10:48:02 UTC

On the topic Fungi, my interest is about this toadstool mushroom,

Amanita muscaria as i think it has a beautiful name such as human name. This mushrooom is one of the most dangerous mushroom. Dr Wan also make a joke about if you all have a child later, you named her as Amanita lol.

If your friend have eaten this poisonous mushroom in the forest, what will you do?

Give him/her charcoals as when we sent him/her to ER, the doctor will give him/her charcoals in a form of tablets.

How can you know if him/her has eaten this poisonous mushroom? So here, I share a website about this.

Newsletter Project Interview

BalqisMustazim — 2016-12-17 11:07:10 UTC

I interview about the multi-degrading microbes which called Burkholderia cepacia strain DRY27 that can degrade both acrylamide and diesel. The level range of temperature from 10 - 40°C.

Acrylamide used in paper industries, agricultural and also oil in mineral processing in form of polymer acrylamide which is called polyacrylamide. It also used in paper industries, it will produce the monomer and waste. In agriculture, the acrylamide used in production of decomposes. It cannot control as they will absorb into the soil. So, we need this microbes to remove acrylamide as it have potential as neurotoxicant so, need to remove them from the environment.

Dr Mohd Tarmizi bin Yusof explained to us, the advantages of Burkholderia cepacia strain DRY27 is suitable for application in the tropics as the level range of temperature for it to react is from 10 - 40°C. Burkholderia cepacia strain DRY27 is locally isolated so it is easier to find this microbes to be used as some has experimented that, paper used to absorb the oil in the river.

Currently, there are no multi-degrading microbes have been reported as commersialised.

Pecha Kucha

BalqisMustazim — 2016-12-17 11:15:04 UTC

My adopted microbes for my PK project is about Bioluminescent. There're two people that presented as same as me but not to same at all. Syahida presented about the Vibrio Ficheri which is the bacterial bioluminiscence that live inside the Bobtail Hawaiian squid meanwhile, Tharashini presented about "Glow in The Dark."

Actually my PK is more on researches and about the dinoflagellate. There is a microbiologist that I admired named Dr. Siouxsie Wiles which also bioluminescent ethuasiast. She deals with this microbes. Specifically, she makes them glow. Why? To save lives.

She said in a talk in TEDxchristchurch, "Out of every four people who are admitted into a hospital overnight in New Zealand are there because of infectious microbes. We are running out of medicines to treat these infections."

At Wiles's lab - the Bioluminescent Superbugs Lab at University Auckland - she and others work to make that task easier, using the light-producing bacteria created via bioluminescent organisms. They use the glowing bacteria like mini torches by putting them inside of animals and they use sensitive cameras to see where they are, how many there are and how changes over time without having to euthanize the animals.

The new journey just started!

BalqisMustazim — 2017-02-15 14:08:29 UTC

There are too many expectations to this new semester. First of all, I want to change myself to be a better person. Achieve the dreams especially to make my parents proud with my own result as I did during primary and secondary school as well. My pointer for first sem is a big regret to myself as other coursemate got better pointer. However, I will try my best for this new semester.
I really hope I could manage time better than last sem. It was a regret when I can't manage my time well as I involved with PALAPES. I did like to join PALAPES since primary school when I was involved in an army camp. We were taught to make singgang batu using batu dalam sungai tu. The taste was incredibly same with singgang ikan . Seriously, it was damn sad when I had to quit PALAPES because of my leg's injuries.

Fighting for the new journey

Which Microbes am I?

BalqisMustazim — 2017-03-02 04:09:22 UTC

We were divided into 8 groups. My group got to do on Gram-Positive bacteria. We made a concept map on that topic using coggle app.

Discussing on topic 1

BalqisMustazim — 2017-03-02 04:13:13 UTC

This day, we discuss about the dichotomous key that we did. Here, I wanna share about bacteria that in the breast milk other than Lactobacillus sp.

My suggestion on VM Local

BalqisMustazim — 2017-04-10 08:16:04 UTC

I suggest Bacillus anthracis. It is a gram-positive bacteria but had been stated in the article year 2001 as the King Agent of Bioterrorism. It is an endospore-forming bacteria. The endospores enter body through inhalation or breaks in skin. This Bacillus anthracis had been used as biological weapons in form of powdered and aerosol form in 5 states which are United Kingdom, Japan, United State, Russia and Iraq.

In 19th century, anthrax involved in several important medical development. The first vaccine containing live organism also discovered in this century which was Louis Pasteur's vet anthrax vaccine.

My Unknown Bacteria (Hands On 1)

BalqisMustazim — 2017-04-10 08:31:02 UTC

First of all, we do the isolation.
From the isolation, we do gram staining.
The picture below is the result of my gram stain.
Next, we do 11 biochemical test which are:

starch hydrolysis,
lipid hydrolysis,
carbohydrate fermentation test,
indole test,
methyl red test,
Voges-Proskauer test,
citrate utilization test,
hydrogen sulfide test,
urease test,
litmus milk reaction,
nitrate reduction test,
catalase test and
oxidase test.

From the result of all the biochemical test, I'm sure that my unknown culture is Alcaligenes faecalis. Alcaligenes faecalis is a gram-negative, rod-shaped bacteria. The cultural characterics that can be seen when this bacteria were isolated in a slant are it is thin, white, spreading and the growth is viscous.

Fungiiiiiiiiiii

BalqisMustazim — 2017-04-10 08:50:20 UTC

It quite funny as we so insecure when handling the fungi as we know it the endospore-forming and might cannot be killed. But, it fun when know that the penicilin that made was from the Penicillium chrysogenum is the hard texture fungi. I had post the picture of the fungi on instagram so, this is their response.

Homesick.

BalqisMustazim — 2017-04-10 09:01:20 UTC

It is about my last paper syndrome. I was really homesick that time. Tetiba, dapat tahu ibu kemalangan. Lagi la rasa nak balik. Apa apapun syukur sebab ibu takde pape cuma kereta je rosak teruk. Alhamdulillah. Cuma trauma la sikit sebab baru je kemalangan.

Finally, I'm home!

BalqisMustazim — 2017-04-10 09:04:25 UTC

Ibu asked me to fry the keropok lekor in the refrigerator. Bukak je peti tengok keropok lekor tu dah ada mold. I refused to fry that. And, my ibu said like this, " Selama belas tahun awak hidup ni, awak beli keropok kat kedai yang dia goreng tu, awak yakin ke yang keropok tu elok. Hidup leklok je awak ibu tengok sampai hari ni." I'm speechless lol. I did snap the pics of the mold but, the pics goneeeee! Maybe I'd delete unconditionally huhu.

AB enzymes

BalqisMustazim — 2017-04-19 06:06:36 UTC

Our first class with Dr Irwan for this semester.

We had to do a 'research' on biotech company in groups. My group did on AB enzymes company.

This AB Enzymes is an industrial biotech company specialized in enzymes and their applications. The company is part of Associated British Foods and represented worldwide. Their offices are located in Brazil, China Germany, Finland, Singapore and USA.

Their products range from food grade enzymes for baking and beverages, grains and vegetable oil seeds processing, to enzymes for animal feed and technical applications: pulp & paper, textiles and laundry detergents.

Here, is the official website of the company:

Different but, not less

BalqisMustazim — 2017-04-19 06:19:18 UTC

Temple Grandin.

Is a really great movie. She's an inspiration *love love love !

She also told the world that women can do what men can do. I also believe that great friends surround her make she trust herself more. It truly heart-breaking when people kept cursing her but, when she told about that to the person that understand her, she become more relax. Especially when her mom told her that she really love her, don't listen to what others thought about her.

Live your life, be yourself. You're pretty amazing.

Dr. Grandin

BalqisMustazim — 2017-04-19 06:46:40 UTC

Mary Temple Grandin is an American professor of animal science at Colorado State University, consultant to the livestock industry on animal behavior, and autism spokesperson.

5th week went welllllll

umimaisyarah2 — 2017-04-19 08:02:26 UTC

It Yakult trip yeay.

We went to Yakult factory in Negeri Sembilan. It was so exciting and a day before, I did some research though on this company. Just a little luls! Yakult is a cultured drink which contains billion of probiotics. In a simple word, probiotics are the good bacteria for your body. Name of the probiotics in the yakult is Lactobacillus shirota strain.

And there, I know something that Yakult also have cosmetic products yo! And, the products are only avail at Japan . The price of the products also pretty expensive for a student like me to afford it uhuhu.

The next day, we went to INTI International University to hear a talk on A surgeon opinion about antibiotic resistant (SUPERBUG).

A little of the important advices that I got is, if you in flu, go see the doctor. Don't share your medicine with anyone. And, if you consume the antibiotic, don't forget to finish it all within the time the Dr asked.

Game on Immunology

BalqisMustazim — 2017-06-19 12:28:36 UTC

On the 12th week, we had game with Dr Irwan which was on Immunology topic. There were so many games that each group made. For examples, snake and ladder, bingo and also musical chair. My group made a game which was bingo. It was so fun and interesting game.

From my point of view, the musical chair game was the fantastic after all. It was really hard to play. But, it was the most interesting game. We all enjoyed the game very much.

My 3MT's

BalqisMustazim — 2017-06-19 12:32:34 UTC

I choose topic for my 3 Minute Talk was about Cholera which is an infectious watery diarrhea. If untreated it may lead to death. In Bahasa Malaysia, Cholera was known as taun. This disease was discovered by a German bacterialogist named Robert Koch. He studied the disease during epidemic in egypt.

For this 3MT, I think I did better than my Pecha Kucha last sem as I prepared well even that morning I got diarrhea. And, present my 3MT in unstable condition huhu. After all, all the presenter were very good.

And, the end of my 2nd Semester

BalqisMustazim — 2017-06-19 12:38:05 UTC

For this semester, I was really sure that did well. But, maybe not for the Microbiology's lab. It is the scariest last paper. I admitted that I was not doing well. I did even better on my 1st semester lab.

By the way, thank you for all lecturers that taught us for this semester. Till we met again next next semester.

Love,
Balqis Mustazim