Group 2 by https://padlet.com/alexandra_zieritz/Group2 en-us 2018-10-03 02:15:33 UTC 2026-01-09 03:39:58 UTC hello@padlet.com Adams et al., 2019 https://padlet.com/alexandra_zieritz/Group2/wish/1320108285 Using eDNA/ metabarcoding as a tool for population genetics - informs conservation management, and is beneficial to both organism and researcher in terms of cutting out risk associated with collecting genetic material

eDNA improves biodiversity estimates due to its high sensitivity- advantages over traditional sampling methods]]> 2021-03-17 11:52:11 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320108285 Trivedi et al 2016 https://padlet.com/alexandra_zieritz/Group2/wish/1320112938 The role of DNA barcoding to effectively undertake biodiversity assessment and conservation of the Earth’s oceans. The identification of cryptic species (species that are morphologically similar but genetically distinct). Faster, higher throughput technique to aid the assessment of global marine biodiversity ]]> 2021-03-17 11:53:46 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320112938 Matthes et al https://padlet.com/alexandra_zieritz/Group2/wish/1320117397 The Barcoding Table of Animal Species (BaTAnS): a new tool to select appropriate methods for animal species identification using DNA barcoding

-new method of data collection/template?
]]> 2021-03-17 11:55:15 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320117397 Zahn et al https://padlet.com/alexandra_zieritz/Group2/wish/1320121607 Development of a DNA mini-barcoding protocol targeting COI for the identification of elasmobranch species in shark cartilage pills

points:
-increased identification of sharks from 36 to 81%
-methodology designed to facilitate conservation
]]> 2021-03-17 11:56:37 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320121607 Question 2: https://padlet.com/alexandra_zieritz/Group2/wish/1320123476 How can and should these methods/tools be used most effectively in biodiversity conservation and biogeographical research? What should future research efforts or collaborations focus on? (research/science)]]> 2021-03-17 11:57:13 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320123476 Future Efforts https://padlet.com/alexandra_zieritz/Group2/wish/1320131551 Should focus on using Metabarcoding as a tool for population genetics

Need to find suitable primers for large groups of taxa - agree universally on which primers/ areas to sample e.g. in plants

Potential for use biogeographically in terms of identifying lineages with genetic variation - could potentially predict which lineages are likely to speciate.

Could quantify effect of environment on genetics/phylogeny by studying species ecotypes.

There should be standardisation of metabarcoding databases so they can be accessed in the same ways, or creation of a global database, so all data can be pooled.
- Databases should be monitored by a group of experts to ensure DNA is correctly matched to taxa.]]> 2021-03-17 11:59:49 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320131551 Liu et al 2014 https://padlet.com/alexandra_zieritz/Group2/wish/1320131788 Used DNA barcoding as a tool for conservation of subtropical Chinese forests. Constructed a barcoding database for 531 tree species to then document the diversity. Barcoding provides an accurate tool for use in biodiversity assessments which then need to be used to enforce conservation ]]> 2021-03-17 11:59:53 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320131788 European Commission https://padlet.com/alexandra_zieritz/Group2/wish/1320158116 Metabarcoding was deemed to be a reliable source of biodiversity monitoring information which can be used by policy makers ]]> 2021-03-17 12:07:39 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320158116 Sigassgaard et al., 2019 https://padlet.com/alexandra_zieritz/Group2/wish/1320163019 Determination of sex ratios of a population through eDNA]]> 2021-03-17 12:08:56 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320163019 Weigand et al 2019 https://padlet.com/alexandra_zieritz/Group2/wish/1320191692 The role of reference libraries and their gaps including recommendations for future work. Found DNA barcode representation of 28,000 aquatic species but found large gaps for diatoms and invertebrates. Need monitoring across more than one country for a species in invertebrate groups. Suggests strategies that should be used to maintain barcode quality of reference libraries. Suggest quality assurance via the process of barcoding and quality control via the cross validation of taxonomic assignments ]]> 2021-03-17 12:16:24 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320191692 Zizka et al., 2020 https://padlet.com/alexandra_zieritz/Group2/wish/1320193191 metabarcoding used to quantify amount of intraspecific variation in a population- useful tool for conservation because it can control ability of a population to adapt to new conditions i.e under climate change
- species with low intraspecific variation could be targeted for more intense conservation/possible translocation to more suitable habitats

Also can be used to identify stressors in freshwater communities- implications for conservation.
Used intraspecific variability against levels of known stress
 Sites with good and stable ecological conditions showed higher intraspecific diversity than stressed sites, which is also coupled with higher OTU diversity. However, due to a low OTU overlap between river systems, genetic diversity analyses were based only on subsets, including all shared OTUs. This subsampling induced the exclusion of variability and ecological specialists, especially at highly diverse sample sites and might have skewed actual differences. Due to these limitations in the underlying data, we cannot disentangle effects of stressors from e.g. population and colonisation and dynamics ]]> 2021-03-17 12:16:47 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320193191 Lui et al https://padlet.com/alexandra_zieritz/Group2/wish/1320203241 The use of DNA barcoding as a tool for
the conservation biogeography of
subtropical forests in China
]]> 2021-03-17 12:19:16 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320203241 West et al., 2021 https://padlet.com/alexandra_zieritz/Group2/wish/1320217690 eDNA was sensitive enough to detect a biogeographic break in marine fish species in Australia.
eDNA- high level of sensitivity thta can discern both fine scale and regional biogeographic patterns]]> 2021-03-17 12:23:01 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320217690 Hobern, 2020 https://padlet.com/alexandra_zieritz/Group2/wish/1320230529 Potential ability to create more accurate estimates of extant species numbers- previously not possible due to the manpower needed to find and identify species.

offers the most flexible tool for recreating biodiversity patterns in time and space

cryptic species- previously been ignored or under represented due to costs associated with identification- metabarcoding can redress these taxonomic biases in conservation


]]> 2021-03-17 12:26:20 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320230529 Meiklejohn 2019 https://padlet.com/alexandra_zieritz/Group2/wish/1320231765 Compared the accuracy and reliability of GenBank and BOLD and found that GenBank outperformed Bold for species level identification for invertebrates, they had similar performance for plants.
GenBank did not underperform.]]> 2021-03-17 12:26:37 UTC https://padlet.com/alexandra_zieritz/Group2/wish/1320231765