https://padlet.com/MedicalBiosciences/6sbfrr34baum en-us 2019-03-14 12:26:21 UTC 2019-03-20 14:50:54 UTC hello@padlet.com https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343355675 dna profile to compare to normal non edited gene]]> 2019-03-20 14:37:37 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343355675 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343355854 Compare proteins synthesis of an unmodified cell to a modified cell ]]> 2019-03-20 14:37:53 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343355854 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343356247 Insert a gene that confers fluorescence or a specific colour]]> 2019-03-20 14:38:26 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343356247 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343356499 Using Markers e.g. fluorescent markers ]]> 2019-03-20 14:38:51 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343356499 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343356893 Add a piece of DNA coding for a florescent gene. Wait for the cells to replicate and see if its fluorescent ]]> 2019-03-20 14:39:11 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343356893 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343357233 Testing for the non-functional or functional protein before and after. If non functional becomes functional or vice versa then it has worked.]]> 2019-03-20 14:39:47 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343357233 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343357384 Using Markers e.g. fluorescent markers]]> 2019-03-20 14:40:02 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343357384 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343357531 Use complementary radioactive or fluorescent DNA probes ]]> 2019-03-20 14:40:18 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343357531 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343357826 Find what protein the gene codes for and then test the modified cell to see whether that protein is still being made. You could also mark the gene with a fluorescent dye. ]]> 2019-03-20 14:40:49 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343357826 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343358125 Grow the CRISPR-edited cells and treat with the antibiotic. All successfully edited cells will die. ]]> 2019-03-20 14:41:15 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343358125 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343358144 Insert fluorescent markers]]> 2019-03-20 14:41:18 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343358144 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343358188 Make a culture from the cells with the knocked out gene, then compare them to a control culture to test if the culture you believe has a knocked out gene expresses the characteristic of the gene or not ]]> 2019-03-20 14:41:22 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343358188 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343358373 Sequence the genome and compare to one that hasn’t been tested on to spot any differences]]> 2019-03-20 14:41:42 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343358373 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343359092 Tag gene of interest with fluorescence ]]> 2019-03-20 14:42:51 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343359092 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343359730 create a culture then take a sample and find the number of bases, compare the number to the number of bases of the control sample]]> 2019-03-20 14:43:54 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343359730 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343361250 2019-03-20 14:46:23 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343361250 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343361783 Unknown repercussions on genetic health (scientific and ethical).]]> 2019-03-20 14:47:19 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343361783 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343362414 2019-03-20 14:48:10 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343362414 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343362433 -Regulation of what is allowed and what isn’t. 
-To what degree is too much.
- Running Clinical Trials - How do you go about it.]]> 2019-03-20 14:48:12 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343362433 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343362748 Ethical issues surrounding changing DNA eg playing God, consent of an embryos/ descendants of someone who has genetically modified DNA 
Reduce Biodiversity 
Unknown effects of genetic change ]]> 2019-03-20 14:48:44 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343362748 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343362895 2019-03-20 14:48:55 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343362895 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343362916 Ethical concerns-designer babies. Religious concerns-playing god. If all diseases were curable it could result in mass overpopulation ]]> 2019-03-20 14:48:56 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343362916 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363124 2019-03-20 14:49:16 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363124 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363247 Unknown effect on epigenetic mechanism]]> 2019-03-20 14:49:27 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363247 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363298 If permanent change to the genetic sequence is off target or ineffective causing an unwanted change, these changes could be passed on to the future generations. 

Also the extent to which genetic editing is ethical in terms of creating a wider socioeconomic gap for those who can afford treatment and those who can’t if designer babies were to become viable. ]]> 2019-03-20 14:49:31 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363298 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363362 Reliability of the techniques - bacteria are different to humans 
Unpredictable outcomes of gene editing
Cost
Ethics - could lead to picked traits and playing God
Not enough testing on the long term effects]]> 2019-03-20 14:49:39 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363362 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363581 Negating the risk of unintended base addition/substitution inbetween  the DNA cut and insertion of new DNA]]> 2019-03-20 14:49:57 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363581 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363670 Lack of consent from both the future child and subsequent generations to which the traits will be passed on.]]> 2019-03-20 14:50:06 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363670 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363820 Designer babies-people being bred only for the purpose of who ever ‘designed’ them e.g. super soldiers etc. 
Economical problems - expensive, if it is used too frequently without success ]]> 2019-03-20 14:50:18 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363820 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363942 Designer babies
Cause unexpected changes

]]> 2019-03-20 14:50:31 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363942 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363965 Ethics of society not being okay with genetic modifications. Unknown long term effects. Hard to actually apply it practically b/c such a small scale. Takes a longggggggg time. Where do the limits lie? Very controversial in general ]]> 2019-03-20 14:50:33 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363965 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363992 Moral - editing the germline of offspring - unfair biological advantage for the rich. Mutations may be amplified in offspring - unknown reprocussions. Ethical - lead to gene-edited babies. ]]> 2019-03-20 14:50:35 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343363992 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343364010 CRISPR was invented for benefit humans and improve their lifestyle, however, CRISPR methods on the human embryo is illegal so how could we overcome this?

Also, Editing DNA especially your gametes could negatively impact on your offsprings and the generations to come.

Cutting edge research therefore it is expensive in terms of equipment and etc.

Also unknown long term effects on the body after CRIPSR editing




]]> 2019-03-20 14:50:37 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343364010 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343364083 Time, money, unexpected consequences on the cells. You might not know of potential side effects on other functions of the cells that may have been affected or other parts of the DNA that may have been affected as well. Ethical issues around animal testing and authorising human tests. Just because it works well on some genes doesn’t mean it will be as efficient on other genes or loci. ]]> 2019-03-20 14:50:43 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343364083 https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343364212 If change in DNA in embryos then the change is passed onto offspring.
Time consuming to incorporate the gene for Cas9]]> 2019-03-20 14:50:54 UTC https://padlet.com/MedicalBiosciences/6sbfrr34baum/wish/343364212