https://padlet.com/spatri11/6dyvqjt24541sgkt en-us 2022-03-24 16:41:05 UTC 2022-04-08 18:32:48 UTC hello@padlet.com spatri11 https://padlet.com/spatri11/6dyvqjt24541sgkt/wish/2136773645 1. make defective retrovirus carrying transgene
2. infect 8-cell stage mouse embryo
3. implant modified embryo into foster mother mouse

https://www.youtube.com/watch?v=82_l5GidwRs]]> 2022-04-08 18:22:02 UTC https://padlet.com/spatri11/6dyvqjt24541sgkt/wish/2136773645 spatri11 https://padlet.com/spatri11/6dyvqjt24541sgkt/wish/2136773867 1. fertilized eggs are flushed from oviducts and immediately microinjected
2. 25-40 eggs are implanted microsurgically into a foster mother that has been made pseudopregnant
3. foster mother will deliver pups from the inoculated eggs about 3 weeks after implantation

https://www.biotecharticles.com/Biotech-Research-Article/Genetic-Transformation-Using-Microinjection-2993.html]]> 2022-04-08 18:22:12 UTC https://padlet.com/spatri11/6dyvqjt24541sgkt/wish/2136773867 spatri11 https://padlet.com/spatri11/6dyvqjt24541sgkt/wish/2136774064 1. Make vector to carry transgene
2. isolate cells from inner cell mass from mouse blastocyst stage embryo (ES cells)
3. transfect ES cells with vector carrying transgene  

https://www.nature.com/articles/d41586-018-06933-w]]> 2022-04-08 18:22:21 UTC https://padlet.com/spatri11/6dyvqjt24541sgkt/wish/2136774064