H.pluvialis by Sema Nur Yıldırım https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55 en-us 2022-10-12 07:37:36 UTC 2023-01-23 12:37:53 UTC hello@padlet.com ssemanuryildirim https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452300134 Strain H2-419-4 of Haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation]]> 2023-01-22 13:19:26 UTC https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452300134 ssemanuryildirim https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452300829 Log fazında üretildikten sonra kimyasallar yardımıyla mutajeneze uğruyor asta biriktirmesi için uzun ve biraz kompleks koşullar var.]]> 2023-01-22 13:21:07 UTC https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452300829 ssemanuryildirim https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452481102 -- The experimental units were then inoculated from an exponentially growing culture with an initial cell density of 10 × 104 cells/mL. All the flasks were kept at a temperature of 24 ± 1 °C, constant aeration (flow rate of 2 L/min), and under an irradiance of 60 μmol photons/m2/s (fluorescent lamps) with continuous illumination.
--Table 1. shows all the composition of all medium.
--The determination of astaxanthin followed the extraction method with hydrochloric acid pretreatment followed by acetone extraction (HCl-ACE) described by Dong et al. (Dong et al. 2014), and the extract obtained was filtered in filter 0.22 μm and analyzed by LC-MS 
--. Different sources of nitrogen (sodium nitrate, ammonium chloride, and urea) on the growth in Haematococcus demonstrated the best results with sodium nitrate (Mazumdar et al. 2019). 
--Higher cell densities (84.50 × 104 cells/mL) and biomass production were observed under mixotrophic conditions in modified Provasoli medium, which besides sodium glycerophosphate, sodium nitrate, and L-asparagine has an addition of yeast extract and organic carbon (sodium acetate) 

]]> 2023-01-22 18:31:17 UTC https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452481102 ssemanuryildirim https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452523235 luvialis NIES-144, which was obtained from the National Institute for Environmental Studies (NIES, Tsukuba, Japan). The seeds were maintained in 250 mL flasks in modified sterilized kobayashi’s basal medium at a constant temperature of 25 ± 1 °C under light intensity of 4±1 μmol photons m−2 s −1 , provided by cool white fluorescent tubes with constant lighting. The modified medium contained 1.2 g CH3COONa, 2.0 g yeast extracts, 0.405 g L-asparagine, 0.2 g MgCl2·6H2O, 0.01 g FeSO4·7H2O, 0.02 g CaCl2·2H2O and 1000 mL distilled water (Kobayashi et al., 2001). The seeds were cultivated until the late exponential growth phase at biomass of 1.0 g L−1 approximately, then harvested by centrifugation in the speed of 3900 rpm for 5 min, and the algal pellets were washed twice by fresh medium for inoculation. ]]> 2023-01-22 19:39:09 UTC https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452523235 ssemanuryildirim https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452525902 Mixo cultivation ile ilgili 4.2 kısmı önemli

Mixotrophic cultivation is considered as the most suitable alternativeto improveHaematococcusgrowth and astaxanthin accumulation.214Various  studies  have  reported  the  superior  performance  ofmixotrophic cultivation ofH.  pluvialisfor astaxanthin production.215–220Nevertheless, the production of astaxanthin fromH. pluvialisis stillvery challenging due to high production cost, low growth rate, highrisk of contamination and extended cultivation period]]> 2023-01-22 19:43:06 UTC https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452525902 ssemanuryildirim https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452539414 2023-01-22 20:07:16 UTC https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452539414 ssemanuryildirim https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452541273 2023-01-22 20:10:49 UTC https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452541273 ssemanuryildirim https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452543052 Fig. 1 önemli

--The strain was grown photoautotrophically in NIES-C medium (pH 7.5) consisting of (in gram per liter unless otherwise stated): Ca(NO 3)2 0.15, KNO 3 0.10, βglycerophosphoric acid disodium salt pentahydrate 0.05, MgSO 4
·7H
2
O 0.04,
Tris-aminomethane 0.50, thiamine 0.01 mg l
−
1
, PIV metal
solution 3.00 ml l
−
1
, biotin 0.10
μ
gl
−
1
, and vitamin B
12
0.10
μ
gl
−
1
. The formulation of PIV metal solution was
Na
2
EDTA 1.0, FeCl
3
·6H
2
O 0.196 and (in milligram per
liter) MnCl
2
·4H
2
O 36.0, ZnSO
4
·7H
2
O 22.0, CoCl
2
·6H
2
O
4.0, and Na
2
MoO
4
·2H
2
O 2.5 (Hata et al.
2001
). 
]]> 2023-01-22 20:14:06 UTC https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452543052 ssemanuryildirim https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452544387 Fig 2 önemli]]> 2023-01-22 20:16:26 UTC https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2452544387 Algal Culture. H. pluVial from Sammlung von Algenkulturen, Pflanzen Physiologisches Institut, Universitat Gottingen, Gottingen, Germany, and grown in an autotrophic medium as detailed in Tripathi et al. (13). The cultures were incubated at 25 (1 °C under a light:dark (16:8 h) cycle with 1.5 klux intensity for aperiod of 1 week. Later, the cultures were incubated at a continuouslight intensity of 3.0 klux with the addition of 0.2% NaCl and 4.4 mM sodium acetate for a period of 2 weeks. The encysted cells whichappeared red due to accumulation of carotenoids were harvested bycentrifugation at 5000 rpm for 5 min at 4 °C. Harvested biomass was lyophilized and used for extraction purposes. ssemanuryildirim https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2453120022 2023-01-23 12:03:22 UTC https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2453120022 https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2453154261 fig 1lere bak. 

]]> 2023-01-23 12:37:46 UTC https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2453154261 ssemanuryildirim https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2453154379 2023-01-23 12:37:53 UTC https://padlet.com/ssemanuryildirim/5ikoy4r53qtpsu55/wish/2453154379