Human Biology: Macromolecule Chemical Test Lab by Cyrus https://padlet.com/cmarkazi/4vpfuqhijm62 By: Cyrus Markazi, Kory Asta, Derek denOuden en-us 2018-05-02 02:06:43 UTC 2023-02-28 07:37:32 UTC hello@padlet.com Purpose cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257092478 This test was used to show us the presence of monosaccharides (simple sugars) in different indigestibles.]]> 2018-05-02 02:21:36 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257092478 Process cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257092632 We were required to use 10 different test tubes, each holding a different food item. We added Benedict's Solution to every test tube and put them in a warm water bath. Some tubes should experience drastic color changes over a period of 3 to 5 minutes from the original blue to red, orange, yellow, green, or brown, signifying the presence of the monosaccharide.

 
]]> 2018-05-02 02:22:30 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257092632 Purpose cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257093126 Each test we completed was used to show us the presence of different nutrients in various foods. ]]> 2018-05-02 02:25:23 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257093126 Process cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257093236 Our group was instructed to complete 5 tests: Benedict's Test, Lugol's Iodine Test, Biuret Test, Brown Paper Test, and Ninhydrin Test.]]> 2018-05-02 02:26:06 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257093236 Actual Procedure Description cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257093805 We gave each test tube a number that correlated with its food item (seen below).

1. Honey
2. Egg White
3. Corn Oil
4. Lettuce
5. Gelatin
6. Butter
7. Potato
8. Apple Juice
9. Distilled Water
10. Unknown #1

Our test was effective. We did see the coloration in some of the tubes, meaning that some ingredients did have the monosaccharide. Unfortunately, however, the colors were not very prominent on our tubes, which was most-likely attributed to us only warming them up for 3 minutes and 50 seconds. Time constraints limited our ability to continue, but our results were adequate enough.]]> 2018-05-02 02:28:59 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257093805 Visual Aid cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257094113 2018-05-02 02:30:47 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257094113 cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257094313 2018-05-02 02:31:41 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257094313 cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257094712 2018-05-02 02:34:10 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257094712 Adding the food items to each test tube cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257094725 2018-05-02 02:34:17 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257094725 cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257094953 2018-05-02 02:35:05 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257094953 Adding the test tubes to the warm water bath cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257094966 2018-05-02 02:35:09 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257094966 Final Results cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257095183 2018-05-02 02:35:53 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257095183 cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257095346 2018-05-02 02:36:44 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257095346 cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257095392 2018-05-02 02:36:58 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257095392 Purpose cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257096380 Lugol's Iodine Test was a process to show us the commonality of polysaccharides (starches) in different food items

Biuret Test was used to identify whether protein was present in each different food.]]> 2018-05-02 02:43:30 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257096380 Results cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257096614 In the end, 3. Corn Oil, 4. Lettuce, 6. Butter, 7. Potato, and 8. Apple Juice experience discoloration; therefore, they have monosaccharides. These will be broken down by enzymes, such as maltase.]]> 2018-05-02 02:44:48 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257096614 Process cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257096942 In both tests, we used a paper to add the solutions onto. We dropped small amount of each food onto the designated spots and then added two drops of each solution into their respective areas. The paper was separated into three columns, the left for the labeled food items, the middle for the Iodine Test, and the right one for Biuret Test  For the Lugol's Iodine Test, we would add acetate on top of the same indigestibles used in the Benedict's Test. Lugol's Iodine Solution's default color is orange. If there was a deep blue or black discoloration, iodine was present in the food item

For the Biuret Test, we added two drops of acetate onto the food. We were looking for the solutions to turn to cloudy pale purple that deviated from the clear color by default for the acetate.]]> 2018-05-02 02:46:23 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257096942 Actual Procedure Description cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257097534 The process was fairly non-problematic. We were able to get results with little issues. ]]> 2018-05-02 02:50:06 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257097534 Visual Aid cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257097863 2018-05-02 02:52:14 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257097863 cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257098772 2018-05-02 02:59:15 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257098772 cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257099786 2018-05-02 03:04:57 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257099786 Biuret Test cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257099809 2018-05-02 03:05:10 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257099809 Lugol's Iodine Test cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257101054 2018-05-02 03:13:10 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257101054 cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257101070 2018-05-02 03:13:20 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257101070 Results cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257101073 Biuret:
Only 3. Corn Oil 6. Butter, and 8. Apple Juice showed positive reactions, a cloudy pale purple. Therefore, they had protein. These would be broken down by enzymes, such as pepsin.

Iodine: 
Every nutrient changed color]]> 2018-05-02 03:13:20 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257101073 Purpose cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257101678 The Brown Paper Test was used to teach us how to find lipids (fats) in different foods.]]> 2018-05-02 03:17:45 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257101678 Process cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257101763 We used brown paper as our canvas, dividing it into 10 different squares for each nutrient. We added 1 drop of each solution onto the paper and waited for it to dry. If there was a see thru spot after drying, that substance has lipids in it.]]> 2018-05-02 03:18:19 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257101763 Actual Procedure Description cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257102027 As easy as the test seemed to be, it was a little difficult, mainly due to the quality of brown paper we used. It is already "spotty," so it was hard to determine which substance generated a translucent spot.]]> 2018-05-02 03:20:05 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257102027 Visual Aid cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257102344 2018-05-02 03:22:29 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257102344 Process cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257102360 We divided pieces of filter paper into 4 quadrants, each designated for their relative food items. We put 2 drops of each solution into its designated area. Then, we added a drop of ninhydrin to to each spot, carefully doing so without touching the solution with our bare skin.]]> 2018-05-02 03:22:37 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257102360 Purpose cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257102374 We used the poisonous substance, ninhydrin, to determine the presence of amino acids in each substance.]]> 2018-05-02 03:22:45 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257102374 cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257355066 2018-05-02 17:05:03 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257355066 cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257355119 2018-05-02 17:05:09 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257355119 cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257355184 2018-05-02 17:05:17 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257355184 cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257355544 2018-05-02 17:05:57 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257355544 Results cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257355702 From the test, we noticed that 3. Corn Oil, 6. Butter, and 8. Apple Juice made the brown paper translucent. These can be broken down by lipase.]]> 2018-05-02 17:06:17 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257355702 Actual Procedure Description cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257408085 The majority of the class used information from one group, as we wanted to minimize exposure to danger. ]]> 2018-05-02 18:49:58 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257408085 Visual Aid cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257410790 2018-05-02 18:56:32 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257410790 cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257414594 2018-05-02 19:05:39 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257414594 Result cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257415220 The food items that showed changes in color were 2. Egg White, 4. Lettuce, 5. Gelatin, 7. Potato. Therefore, these materials had amino acids, which can be broken down by carboxypeptidase.]]> 2018-05-02 19:06:59 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257415220 ddenouden https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257619180 2018-05-03 12:45:33 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257619180 ddenouden https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257619622 2018-05-03 12:46:33 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257619622 ddenouden https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257619923 2018-05-03 12:47:15 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257619923 ddenouden https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257620199 2018-05-03 12:47:53 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257620199 ddenouden https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257620530 2018-05-03 12:48:36 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257620530 Various Food Items cmarkazi https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257701890 2018-05-03 15:14:50 UTC https://padlet.com/cmarkazi/4vpfuqhijm62/wish/257701890