Ion-Exchange Chromatography by Akima Kamen https://padlet.com/kamenakima/42nv49753yya en-us 2018-02-22 06:55:48 UTC 2023-04-23 04:00:34 UTC hello@padlet.com kamenakima https://padlet.com/kamenakima/42nv49753yya/wish/234096988 Ion exchange chromatography: Ion-exchange chromatography is a versatile, high resolution chromatography techniques to purify the protein from a complex mixture. In addition, this chromatography has a high loading capacity to handle large sample volume and the chromatography operation is very simple.
 Principle: This chromatography distributes the analyte molecule as per charge and their affinity towards the oppositively charged matrix. The analytes bound to the matrix are exchanged with a competitive counter ion to elute. The interaction between matrix and analyte is determined by net charge, ionic strength and pH of the buffer. 
]]> 2018-02-22 07:15:34 UTC https://padlet.com/kamenakima/42nv49753yya/wish/234096988 Hence, there are two types of ion-exchange chromatography: kamenakima https://padlet.com/kamenakima/42nv49753yya/wish/234097480 1.Cation exchange chromatography- In cation exchange chromatography, matrix has a negatively charged functional group with a affinity towards positively charged molecules.The positively charged analyte replaces the reversible bound cation and binds to the matrix In the presence of a strong cation (such as Na+) in the mobile phase, the matrix bound positively charged analyte is replaced with the elution of analyte.
2.Anion Exchange chromatography- In anion exchange chromatography, matrix has a positively charged functional group with a affinity towards negatively charged molecules. The negatively charged analyte replaces the reversible bound anion and binds to the matrix (Figure 30.2, B). In the presence of a strong anion (such as Cl-) in the mobile phase, the matrix bound negatively charged analyte is replaced with the elution of analyte.]]> 2018-02-22 07:19:28 UTC https://padlet.com/kamenakima/42nv49753yya/wish/234097480