Module 4_G2 by Laura Bacete https://padlet.com/laurabacete/BI2015_G2_M4 What should be in the report? en-us 2021-10-18 10:18:45 UTC 2025-11-11 04:55:56 UTC hello@padlet.com in material collection step 2-3: 20-30 seedlings put in dishes of DMSO/ISX for microscopy https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832905002 2021-10-21 08:42:34 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832905002 Phytohormones https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832928970 2021-10-21 08:56:06 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832928970 Arabidopsis as a plant mode system https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832930129 2021-10-21 08:56:45 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832930129 https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832930358 Model systems]]> 2021-10-21 08:56:54 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832930358 Different stresses plants can be exposed to https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832931272 2021-10-21 08:57:25 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832931272 https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832931310 Cell wall integrity, stress response, lignin, regulation of gene expression in stress response, phytohormones]]> 2021-10-21 08:57:26 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832931310 isx https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832931322 2021-10-21 08:57:26 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832931322 Lignin https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832931643 2021-10-21 08:57:37 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832931643 https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832931713 Cell wall integrity systems 
Arabidopsis 
QtPCR
LIquid chrom
Mass spectrometry]]> 2021-10-21 08:57:40 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832931713 plants dont move, everyone https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832932170 2021-10-21 08:57:56 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832932170 How qRT-PCR works https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832932377 2021-10-21 08:58:03 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832932377 qtRT-PCR https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832932462 2021-10-21 08:58:04 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832932462 Wiesner reaction https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832932811 2021-10-21 08:58:15 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832932811 https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832934067 Present each of the three results (q-RT PCR, microscopy, LC-MS)]]> 2021-10-21 08:58:57 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832934067 Pictures of roots https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832934123 2021-10-21 08:58:59 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832934123 Analysis of microscopy images https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832934469 2021-10-21 08:59:10 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832934469 Results from phytohormone purification https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832935774 2021-10-21 08:59:54 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832935774 Different phenotypes https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832936484 2021-10-21 09:00:07 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832936484 https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832936765 Microscopy]]> 2021-10-21 09:00:16 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832936765 What do you expect? https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832937010 2021-10-21 09:00:25 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832937010 hypothesis https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832937076 2021-10-21 09:00:28 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832937076 https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832937463 Discuss the genes and the effect of ISX and DMSO ]]> 2021-10-21 09:00:41 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832937463 Data analysis https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832937671 2021-10-21 09:00:48 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832937671 Statistics https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832938218 2021-10-21 09:01:08 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832938218 https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832938289 Changes in rna-isolation]]> 2021-10-21 09:01:10 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832938289 https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832938748 Mounting of seedlings]]> 2021-10-21 09:01:26 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832938748 Mounting on object slide directly in pg-HCl instead of EtOH https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832939566 2021-10-21 09:01:55 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832939566 microscope model + settings etc https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832940708 2021-10-21 09:02:34 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832940708 What you do in ImageJ, settings on microscope https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832941125 2021-10-21 09:02:48 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832941125 https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832941456 Discuss phytohormone results]]> 2021-10-21 09:03:01 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832941456 Where the results as expected? If not, why? https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832941856 2021-10-21 09:03:16 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832941856 Discuss additions you could do to get more concrete results https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832942261 2021-10-21 09:03:31 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832942261 Squeezed roots affect results, how could it have been avoided? https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832943485 2021-10-21 09:04:14 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832943485 https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832943932 What could be better, ex. not to squeeze the roots]]> 2021-10-21 09:04:29 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832943932 qRT-PCR results https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832944218 2021-10-21 09:04:41 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832944218 Size of dataset https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832944566 2021-10-21 09:04:55 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832944566 https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832945708 Answer hypothesis]]> 2021-10-21 09:05:40 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832945708 https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832947752 Do plants percieve stress?]]> 2021-10-21 09:06:56 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832947752 https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832954833 Discuss the roots in microscopy, how did it affect the imaging and detection of redness if the roots were dry? ]]> 2021-10-21 09:11:20 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832954833 use journal club report https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832956241 2021-10-21 09:12:13 UTC https://padlet.com/laurabacete/BI2015_G2_M4/wish/1832956241